150 Scientific Articles Published on the Health Benefits of Black Seed and Black Seed Oil (Nigella s


[1] A systematic review and meta-analysis of the effect of Nigella sativa supplementation on obesity indices

Abstract Title:

Effect of Nigella sativa supplementation on obesity indices: A systematic review and meta-analysis of randomized controlled trials.

Abstract Source:

Complement Ther Med. 2018 Jun ;38:48-57. Epub 2018 Apr 11. PMID: 29857879

Abstract Author(s):

Seyed Mohammad Mousavi, Ali Sheikhi, Hamed Kord Varkaneh, Meysam Zarezadeh, Jamal Rahmani, Alireza Milajerdi

Article Affiliation:

Seyed Mohammad Mousavi

Abstract:

BACKGROUND & OBJECTIVE(S): No meta-analysis is available on the effect of Nigella sativa (NS) on obesity indices. This systematic review and meta-analysis was conducted to systematically review the available Randomized Clinical Trials (RCTs) that examined the effects of NS on Body Weight (BW), Body Mass index (BMI), and Waist Circumference (WC) in adults.

METHODS: Relevant articles published up to January 2018 were searched through PubMed/Medline, SCOPUS, Cochrane Library, and Google Scholar databases, using relevant keywords. All RCTs that examined the effect of NS supplementation on BW, BMI, or WC were included.

RESULTS: Overall, thirteen RCTs, including 875 subjects (64% males) were included in this study. Combining effect sizes from ten studies, NS supplementation significantly reduced BW (Weighted Mean Differences (WMD): -1.76 kg, 95% CI: -3.34 to -0.17, I = 87.4%), as compared to placebo. Subgroup analysis by the intervention type (I = 0.0%), participants' gender (I = 0.0%), and age (I = 5.5%) removed between-study heterogeneity. A significant reduction was seen in BMI (WMD: -0.85 kg/m, 95% CI: -1.23, -0.46, I = 70.6%) after NS supplementation than placebo among eleven trials. Subgroup analysis based on study duration (I = 0.0%), participants' gender (females: I = 0.0%&both genders: I = 20.9%), an age (I = 35.9%) disappeared the heterogeneity. However, no significant reduction was found in WC comparing NS supplementation to placebo (WMD: -4.04 cm, 95% CI: 11.37, 3.27, I = 97.8%) in five studies.

CONCLUSIONS: We find a significant effect of NS supplementation on BW and BMI in adults. However, the effect of NS supplementation on WC was not significant in this meta-analysis.

Article Published Date : May 31, 2018

Study Type : Meta Analysis, Review

[2] Current findings suggest N. sativa supplementation a suitable choice in managing the complications of T2D

Abstract Title:

Nigella sativa improves glucose homeostasis and serum lipids in type 2 diabetes: A systematic review and meta-analysis.

Abstract Source:

Complement Ther Med. 2017 Dec ;35:6-13. Epub 2017 Aug 30. PMID: 29154069

Abstract Author(s):

Reza Daryabeygi-Khotbehsara, Mahdieh Golzarand, Mohammad Payam Ghaffari, Kurosh Djafarian

Article Affiliation:

Reza Daryabeygi-Khotbehsara

Abstract:

BACKGROUND & OBJECTIVE(S): Global prevalence of type 2 diabetes (T2D) is very high and is currently growing alarmingly. With respect to recent researchers' attention to the potential role of herbal medicine in disease prevention and management, the present meta-analysis review investigates the effectiveness of Nigella sativa (N. sativa), a popular herb, in T2D.

METHODS: Literature search was conducted covering PubMed/Medline, Scopus, and Cochrane Registry of Clinical Trials up to February 2017 to obtain the relevant published intervention studies. Study selection, quality rating and data extraction of studies were investigated by two independent reviewers. Heterogeneity was assessed using I-squared (I2) statistics test. Subgroup analysis was done to assess type of N. sativa supplement as source of heterogeneity. Effect sizes of eligible studies were pooled using STATA software version 12 (STATA corp, College Station, TX, USA).

RESULTS: Seven trials were included in the meta-analysis of glycemic and serum lipid profile end points. Supplementation with N. sativa significantly improved fasting blood sugar (FBS) [-17.84mg/dl, 95% CI: -21.19 to -14.49, p<0.001], HbA1c [-0.71%, 95% CI: -1.04 to -0.39, p<0.001], total-cholesterol (TC) [WMD: -22.99mg/dl, 95% CI: -32.16 to -13.83, p<0.001] and LDL-cholesterol (LDL-c) [-22.38mg/dl, 95% CI: -33.60 to -11.15, p<0.001]. The overall effects for triglyceride (TG) [-6.80mg/dl, 95% CI: -33.59 to 19.99, p=0.61] and HDL-cholesterol (HDL-c) [0.37mg/dl, 95% CI: -1.59 to 2.33, p=0.71] were insignificant. Subgroup analysis revealed significant reduction on TG with N. sativa seed oil [-14.8mg/dl, 95% CI: -23.1 to -6.5, p<0.001], while TG was increased with seed powder [29.4mg/dl, 95% CI: 16.9-42.0, p<0.001]. All measures, but HbA1c, showed no evidence of publication bias.

CONCLUSION: Although, the meta-analysis conducted included a few number of studies, but has shown promising results on the effectiveness of N. sativa on glucose homeostasis and serum lipids. Current findings suggest N. sativa supplementation a suitable choice in managing the complications of T2D, although future researches are necessary.

Article Published Date : Nov 30, 2017

Study Type : Meta Analysis, Review

[3] Nigella Sativa has a significant impact on plasma lipid concentrations, leading to lower total cholesterol

Abstract Title:

Nigella Sativa (Black Seed) Effects on Plasma Lipid Concentrations in Humans: a Systematic Review and Meta-Analysis of Randomized Placebo-Controlled Trials.

Abstract Source:

Pharmacol Res. 2016 Feb 10. Epub 2016 Feb 10. PMID: 26875640

Abstract Author(s):

Amirhossein Sahebkar, Guglielmo Beccuti, Luis E Simental-Mendía, Valerio Nobili, Simona Bo

Article Affiliation:

Amirhossein Sahebkar

Abstract:

The effects of Nigella Sativa (NS) on plasma lipid concentrations are controversial. A systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted to obtain a conclusive result in humans. PubMed-Medline, SCOPUS, Web of Science, and Google Scholar databases were searched (up to August 2015) to identify RCTs investigating the impact of NS on total cholesterol, LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C), and triglycerides concentrations. A random-effects model and the generic inverse variance weighting method were used for quantitative data synthesis. Meta-regression, sensitivity analysis, and publication bias assessments were performed using standard methods. A total of 17 RCTs examining the effects of NS on plasma lipid concentrations were included. Meta-analysis suggested a significant association between NS supplementation and a reduction in total cholesterol (weighed-mean-difference [WMD]: -15.65mg/dL, 95% CI: -24.67, -6.63, p=0.001), LDL-C (WMD: -14.10mg/dL, 95% CI: -19.32, -8.88, p<0.001), and triglyceride levels (WMD: -20.64mg/dL, 95% CI: -30.29, -11.00, p<0.001). No significant effect on HDL-C concentrations (WMD: 0.28mg/dL, 95% CI: -1.96, 2.53, p=0.804) was found. A greater effect of NS seed oil versus seed powder was observed on serum total cholesterol and LDL-C levels, and an increase in HDL-C levels was found only after NS seed powder supplementation. NS has a significant impact on plasma lipid concentrations, leading to lower total cholesterol, LDL-C, and TG levels while increased HDL-C is associated with NS powder only. Further RCTs are needed to explore the NS benefits on cardiovascular outcomes.

Article Published Date : Feb 09, 2016

Study Type : Meta Analysis, Review

[4] Supplementation with N. sativa exerts a moderate effect on reduction in body weight, body mass index and waist circumference

Abstract Title:

The effects of Nigella sativa L. on obesity: A systematic review and meta-analysis.

Abstract Source:

J Ethnopharmacol. 2018 Mar 17. Epub 2018 Mar 17. PMID: 29559374

Abstract Author(s):

Nazli Namazi, Bagher Larijani, Mohammad Hossein Ayati, Mohammad Abdollahi

Article Affiliation:

Nazli Namazi

Abstract:

ETHNOPHARMACOLOGICAL RELEVANCE: Nigella sativa L. (N.sativa) is a traditional herbal medicine that has been used for centuries to treat rheumatoid arthritis, diabetes, asthma, and other metabolic disorders. Recently, anti-obesity characteristics of N.sativa have been indicated.

AIM OF THE STUDY: The effects of N. sativa as a complementary therapy in obesity management remains controversial. We aimed to perform a meta-analysis on the effects of supplementation with N. sativa on some anthropometric indices in adult subjects.

MATERIALS AND METHODS: We searched PubMed/Medline, Cochrane Library, ISI Web of Science, and Scopus databases untill June 2017 to identify relevant placebo-controlled clinical trials. Data was reported as weighted mean differences and standard deviations to show the magnitude of effects for N. sativa on body weight, body mass index (BMI) and waist circumference (WC).

RESULTS: Findings of 11 studies revealed that N. sativa supplement reduced body weight (-2.11kg, 95% CI: -3.61, -0.61, I:72.4%), BMI (-1.16kg/m; 95%CI: -1.81, -0.51; I: 40.1%) and WC (-3.52cm, 95%CI: -4.10, -2.92, I=0%) significantly compared to placebo groups.

CONCLUSION: Supplementation with N. sativa exerts a moderate effect on reduction in body weight, BMI and WC. However, due to the high heterogeneity for body weight and limited high quality studies, the findings should be declared by caution. No serious side effects were also reported following N. sativa supplementation. Further studies are needed to clarify the effects of N. sativa on other anthropometric indices.

Article Published Date : Mar 16, 2018

[5] This meta-analysis suggests that short-term treatment with N. sativa powder can significantly reduce SBP and DBP levels

Abstract Title:

A systematic review and meta-analysis of randomized controlled trials investigating the effects of supplementation with Nigella sativa (black seed) on blood pressure.

Abstract Source:

J Hypertens. 2016 Aug 10. Epub 2016 Aug 10. PMID: 27512971

Abstract Author(s):

Amirhossein Sahebkar, Davide Soranna, Xiaoqiu Liu, Costas Thomopoulos, Luis E Simental-Mendia, Giuseppe Derosa, Pamela Maffioli, Gianfranco Parati

Article Affiliation:

Amirhossein Sahebkar

Abstract:

Several reports suggest a beneficial effect of Nigella sativa on cardiometabolic risk factors, including blood pressure (BP). However, the magnitude of the BP-lowering effect of N. sativa remains controversial. We aimed to calculate this effect size of N. sativa through a systematic review and meta-analysis of randomized controlled trials (RCTs). PubMed, Cochrane Collaboration Library, and SCOPUS were used as data sources of RCTs published before 30 August 2015 (in English only) comparing N. sativa treatment with placebo or standard treatment. We used random effect models to estimate the difference in BP reductions, I index andχ statistics to measure and test the interstudy heterogeneity. Random effect metaregression models were applied to explore the associations between BP reduction and duration of treatment, dosage of N. sativa, and type of black seed as potential confounders. Publication bias was evaluated using funnel plot and Egger's regression asymmetry test. Total 11 RCTs, including 860 hypertensive or normotensive individuals were eligible, of which 10 compared N. sativa versus placebo and one versus standard treatment. SBP and DBP means decreased from 132.85 to 125.19 mmHg and from 82.63 to 77.74 mmHgafter mean treatment duration of 8.3 weeks in N. sativa groups, respectively. The difference in reductions as compared with control/standard groups were -3.26 (-5.10, -1.42; I = 59%) mmHg in SBP and -2.80 (-4.28, -1.32; I = 60%) mmHg in DBP. Pharmaceutical preparations of N. sativa in powder and oil demonstrated a different lowering effect (in favour of the former) on both SBP and DBP. No association was observed between SBP lowering and time on treatment, N. sativa dosage or type of N. sativa. The results of Egger's test did not indicate any publication bias. Our meta-analysis suggests that short-term treatment with N. sativa powder can significantly reduce SBP and DBP levels. More evidence is needed, however, to strengthen the available evidence and to further explore the long-term BP-lowering effect of N. sativa.

Article Published Date : Aug 09, 2016

Study Type : Meta Analysis, Review

[6] Black cumin (Nigella sativa) and Phyllanthus niruri provide symptomatic relief in the treatment of acute tonsillo-pharyngitis patients

Abstract Title:

Symptomatic treatment of acute tonsillo-pharyngitis patients with a combination of Nigella sativa and Phyllanthus niruri extract.

Abstract Source:

Int J Clin Pharmacol Ther. 2008 Jun;46(6):295-306. PMID: 18541126

Abstract Author(s):

M Dirjomuljono, I Kristyono, R R Tjandrawinata, D Nofiarny

Article Affiliation:

Department of Ear, Nose, Throat, Faculty of Medicine, University of Airlangga/Dr. Soetomo General Hospital, Surabaya, Jakarta, Indonesia. eleanora.anggiara@dexa-media.com

Abstract:

Acute tonsillopharyngitis is characterized by tonsil or pharyngeal inflammation and mostly is a virus in origin; thus, treatment that covers both the inflammation and inadequate immune response against the pathogenic organism is needed. NSPN extract containing Nigella sativa and Phyllanthus niruri extracts has both antiinflammatory and immunomodulatory effects. A comparative, parallel, randomized, double-blind, placebo-controlled study with a treatment period of 7 days was conducted to examine clinical effectiveness of Nigella sativa and Phyllanthus niruri extract (NSPN extract). Of 200 enrolled patients, 186 patients completed the study, 12 patients withdrew and 2 patients were principally screened failure but inadvertently included. NSPN capsules, each containing 360 mg Nigella sativa and 50 mg Phyllanthus niruri extracts, were orally administered 3 times 1 capsule daily for 7 days. At Hour 5 or 6 of the first dosing of study medication, the sore throat assessed as swallowing pain and difficulty, was markedly alleviated in the NSPN group. In line with the significant alleviation of pain, from Days 0 to 2 of treatment, subjects in the NSPN group also needed significantly less escapeâanalgesicâ therapy (paracetamol tablets) than those in the placebo group. At the end of treatment (Day 7), a significantly greater proportion of patients in the NSPN group than in the placebo group had their sore throat completely relieved. NSPN extract was also found to be safe and well tolerated in acute tonsillopharyngitis patients. This study proved significant benefits of NSPN extract in the treatment of acute tonsillopharyngitis as compared to placebo.

Article Published Date : Jun 01, 2008

Study Type : Human Study

[7] Black cumin (Nigella sativa) has a prophylactic effect on chemical war victims

Abstract Title:

The possible prophylactic effect of Nigella sativa seed aqueous extract on respiratory symptoms and pulmonary function tests on chemical war victims: a randomized, double-blind, placebo-controlled trial.

Abstract Source:

J Altern Complement Med. 2008 Nov;14(9):1137-44. PMID: 18991514

Abstract Author(s):

Mohammad H Boskabady, Javad Farhadi

Abstract:

BACKGROUND: In previous studies, the relaxant, anticholinergic (functional antagonism), and antihistaminic effects of various extracts and oils of Nigella sativa seed have been demonstrated. In the present study, the prophylactic effect of a boiled aqueous extract of nigella seed on chemical war victims was examined. METHODS: Forty (40) chemical war victims were randomly divided into control group (20 patients) and study group (20 patients), and they were studied for 2 months. In the study group 0.375 mL/kg of 50 g% boiled extract and in the control group a placebo solution were administered daily throughout the study. Respiratory symptom score and wheezing were recorded in the beginning (first visit), 30 days after treatment (second visit), and at the end of the study (third visit). Pulmonary function tests (PFTs) were also measured, and the drug regimen of the patients was evaluated at 3 different visits. RESULTS: All respiratory symptoms, chest wheezing, and PFT values in the study group significantly improved in the second and third visits compared to the first visit (p < 0.05 to p < 0.001). In addition, further improvement of chest wheezing and some PFT values on the third visit were observed compared to the second visit in this group (p < 0.05 to p < 0.001). In the third visits, all PFT values and most symptoms in the study group were significantly different from those of the control group (p < 0.01 to p < 0.001). However, in the control group, there were only small improvements in some parameters in the second and third visits. The use of inhaler and oral beta-agonists and oral corticosteroid in the study group decreased at the end of the study, while there were no obvious changes in use of the drugs in control subjects. CONCLUSIONS: The results of this study suggest a prophylactic effect of N. sativa on chemical war victims and warrant further research regarding this effect.

Article Published Date : Nov 01, 2008

Study Type : Human Study

[8] Black cumin (Nigella sativa) is effective in long-term treatment of opioid dependence

Abstract Title:

A new and novel treatment of opioid dependence: Nigella sativa 500 mg.

Abstract Source:

J Ayub Med Coll Abbottabad. 2008 Apr-Jun;20(2):118-24. PMID: 19385474

Abstract Author(s):

Sibgplah Sangi, Shahida P Ahmed, Muhammad Aslam Channa, Muhammad Ashfaq, Shah Murad Mastoi

Abstract:

BACKGROUND: Opioid dependence is one of the major social and psychiatric problem of society. Unfortunately there is no non opiate treatment available. For centuries man has used plants for their healing proprieties. These plants play a fundamental part in all treatment modalities, both ancient and modern. METHODS: This study was conducted to find non opiate treatment for opiate withdrawal. Total 35 known addicts of opiates were included in the study. This study was based on DSM IV criteria for opioid dependence. RESULT: This study demonstrates that non opioid treatment for opioid addiction decreases the withdrawal effects significantly. It further demonstrates that there are no changes in physiological parameters of subjects during treatment (BP, Pulse rate etc.). There is increased appetite but no significant weight gain in the subjects. CONCLUSION: Non opioid drug Nigella sativa is effective in long-term treatment of opioid dependence. It not merely cures the opioid dependence but also cures the infections and weakness from which majority of addicts suffer.

Article Published Date : Apr 01, 2008

Study Type : Human Study

[9] Black cumin alleviates symptoms of allergic rhinitis

Abstract Title:

Herbal treatment of allergic rhinitis: the use of Nigella sativa.

Abstract Source:

Am J Otolaryngol. 2010 Oct 12. Epub 2010 Oct 12. PMID: 20947211

Abstract Author(s):

Soheila Nikakhlagh, Fakher Rahim, Faezeh Hossein Nejad Aryani, Amir Syahpoush, Mehri Ghafouryan Brougerdnya, Nader Saki

Article Affiliation:

Departments of ENT, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abstract:

BACKGROUND AND AIMS: Allergic rhinitis is the most common chronic and allergic disease, especially in children. This study aimed to investigate the anti-inflammatory effects of Nigella sativa and its effects on inflammatory factors in patients with allergic rhinitis symptoms and the process their clinical study charges. SETTING: The present study is a clinical trial that conducted as prospective and double blind with descriptive analytic. MATERIALS AND METHODS: The sample included 66 patients (case and placebo) with allergic rhinitis exposed to N. sativa oil. Individual characteristics, including age and sex, and characteristics of the disease, including nasal congestion, runny nose, itchy nose, and sneezing attacks, were evaluated. From the start of the study, that is, day 0, up to the end of the study, that is, day 30, an observer completed the symptoms severity questionnaire. STATISTICAL ANALYSIS: Data were presented as means± SEM. Comparisons between groups were performed by using paired Student t test. Differences were considered significant if P values are less than .05 and .01. RESULTS: In the present study, 66 patients with allergic rhinitis, including 22 males (33.3%) and 44 females (66.7%) with a mean age of 47.19 years, were included. Immunoglobulin E total of more than 100 was reported in 38 patients before treatment. Immunoglobulin E in nasal wash from 7 patients was observed and was not measurable in 59 cases. Only 6.1% of the study population had nasal mucosal eosinophil. CONCLUSION: The results showthat N. sativa could reduce the presence of the nasal mucosal congestion, nasal itching, runny nose, sneezing attacks, turbinate hypertrophy, and mucosal pallor during the first 2 weeks (day 15). The present findings are consistent with evidence that the antiallergic effects of N. sativa componentscould be attributed to allergic rhinitis. Moreover, N. sativa should be considered for treating allergic rhinitis when the effects of other antiallergic drugs need to be avoided.

Article Published Date : Oct 12, 2010

Study Type : Human Study

[10] Black cumin possesses clinical useful anti-H. pylori activity

Abstract Title:

Comparative study of Nigella Sativa and triple therapy in eradication of Helicobacter Pylori in patients with non-ulcer dyspepsia.

Abstract Source:

Saudi J Gastroenterol. 2010 Jul-Sep;16(3):207-14. PMID: 20616418

Abstract Author(s):

Eyad M Salem, Talay Yar, Abdullah O Bamosa, Abdulaziz Al-Quorain, Mohamed I Yasawy, Raed M Alsulaiman, Muhammad A Randhawa

Article Affiliation:

Department of Physiology, College of Medicine, King Faisal University, Dammam, Saudi Arabia.

Abstract:

BACKGROUND/AIM: A large number of diseases are ascribed to Helicobacter pylori (H. pylori), particularly chronic active gastritis, peptic ulcer disease and gastric cancer. Successful treatment of H. pylori infection with antimicrobial agents can lead to regression of H. pylori-associated disorders. Antibiotic resistance against H. pylori is increasing, and it is necessary to find new effective agents. Nigella sativa seed (NS), a commonly used herb, possesses in vitro anti-helicobacter activity. The present study was undertaken to evaluate the efficacy of NS in eradication of H. pylori infection in non-ulcer dyspeptic patients. MATERIALS AND METHODS: The study was conducted on 88 adult patients attending King Fahd Hospital of the University, Al-Khobar, Saudi Arabia, from 2007 to 2008, with dyspeptic symptoms and found positive for H. pylori infection by histopathology and urease test. Patients were randomly assigned to four groups, receiving i) triple therapy (TT) comprising of clarithromycin, amoxicillin, omeprazole [n= 23], ii) 1 g NS + 40 mg omeprazole (OM) [n= 21], iii) 2 g NS + OM [n= 21] or iv) 3 g NS + OM [n= 23]. Negative H. pylori stool antigen test four weeks after end of treatment was considered as eradication. RESULTS: H. pylori eradication was 82.6, 47.6, 66.7 and 47.8% with TT, 1 g NS, 2 g NS and 3 g NS, respectively. Eradication rates with 2 g NS and TT were statistically not different from each other, whereas H. pylori eradication with other doses was significantly less than that with TT (P<0.05). Dyspepsia symptoms improved in all groups to a similar extent. CONCLUSIONS: N. sativa seeds possess clinically useful anti-H. pylori activity, comparable to triple therapy. Further clinical studies combining N. sativa with antibiotics are suggested.

Article Published Date : Jul 01, 2010

Study Type : Human Study

[11] Black cumin seeds decreased Methotrexate hepatotoxicity and improved survival in children with ALL

Abstract Title:

Therapeutic value of Black seed oil in Methotrexate Hepatotoxicity in Egyptian Children with Acute Lymphoblastic Leukemia.

Abstract Source:

Infect Disord Drug Targets. 2015 Mar 20. Epub 2015 Mar 20. PMID: 25809628

Abstract Author(s):

Adel A Hagag, Ahmed M Abd Elaal, Mohamed S Elfaragy, Samir M Hassan, Enas A Elzamarany

Article Affiliation:

Adel A Hagag

Abstract:

BACKGROUND: 'Acute lymphoblastic leukemia is the most common childhood malignancies'. Adding methotrexate to treatment protocols increased survival rate in children with leukemia. Methotrexate efficacy is limited by its hepatotoxicity. 'Aim of the study: To assess' the therapeutic value of Black seed oil in 'methotrexate induced hepatotoxicity in Egyptian children with acute lymphoblastic leukemia '. 'Subjects and methods: This study was conducted on 40 children with acute lymphoblastic leukemia' including 20 patients under methotrexate therapy and Black seeds 80mg/kg/day for one week after each methotrexate dose (Group II) and 20 patients under methotrexate and placebo (Group III).This study included also '20 healthy children as a control group' (Group I). All patients were subjected to complete blood picture, bone marrow aspiration and liver functions.

RESULTS: No significant differences in liver functions between group II and III before therapy. There were non-significant increase in liver functions in group II after therapy but there was significant increase in group III with significant difference between group II and III after therapy. There were significant differences in prognosis regarding remission, relapse, death and 'disease free survival but no significant difference in overall survival between group II and III'.

CONCLUSION: Black cumin seeds decreased MTX hepatotoxicity and improved survival in children with ALL and can be recommended as adjuvant drug in patients with ALL under methotrexate therapy.

Article Published Date : Mar 19, 2015

Study Type : Human Study

[12] Black cumin seeds have significant anti-diabetic effects in type 2 diabetics

Abstract Title:

Effect of Nigella sativa seeds on the glycemic control of patients with type 2 diabetes mellitus.

Abstract Source:

Indian J Physiol Pharmacol. 2010 Oct-Dec;54(4):344-54. PMID: 21675032

Abstract Author(s):

Abdullah O Bamosa, Huda Kaatabi, Fatma M Lebdaa, Abdul-Muhssen Al Elq, Ali Al-Sultanb

Article Affiliation:

Department of Physiology, College of Medicine, King Faisal University, Dammam, Saudi Arabia. aosbamosa@gmail.com

Abstract:

Diabetes mellitus is a common chronic disease affecting millions of people world wide. Standard treatment is failing to achieve required correction of blood glucose in many patients. Therefore, there is a need for investigating potential hypoglycemic drugs or herbs to improve glycemic control in diabetic patients. Nigella sativa seeds were used as an adjuvant therapy in patients with diabetes mellitus type 2 added to their anti-diabetic medications. A total of 94 patient were recruited and divided randomly into three dose groups. Capsules containing Nigella sativa were administered orally in a dose of 1, 2 and 3 gm/day for three months. The effect of Nigella sativa on the glycemic control was assessed through measurement of fasting blood glucose (FBG), blood glucose level 2 hours postprandially (2 hPG), and glycosylated hemoglobin (HbA1c). Serum C-peptide and changes in body weight were also measured. Insulin resistance and beta-cell function were calculated usin the homeostatic model assessment (HOMA2). Nigella sativa at a dose of 2 gm/day caused significant reductions in FBG, 2hPG, and HbA1 without significant change in body weight. Fasting blood glucose was reduced by an average of 45, 62 and 56 mg/dl at 4, 8 and 12 weeks respectively. HbAlC was reduced by 1.52% at the end of the 12 weeks of treatment (P<0.0001). Insulin resistance calculated by HOMA2 was reduced significantly (P<0.01), while B-cell function was increased (P<0.02) at 12 weeks of treatment. The use of Nigella sativa in a dose of 1 gm/day also showed trends in improvement in all the measured parameters but it was not statistically significant from the baseline. However, no further increment in the beneficial response was observed with the 3 gm/day dose. The three doses of Nigella sativa used in the study did not adversely affect either renal functions or hepatic functions of the diabetic patients throughout the study period. In Conclusion: the results of this study indicate that a dose of 2 gm/ day of Nigella sativa might be a beneficial adjuvant to oral hypoglycemic agents in type 2 diabetic patients.

Article Published Date : Oct 01, 2010

Study Type : Human Study

[13] Black cumin seeds improves serum lipids, atherogenic index of plasma and modulates anthropometric features in patients with Hashimoto's thyroiditis

Abstract Title:

Powdered black cumin seeds strongly improves serum lipids, atherogenic index of plasma and modulates anthropometric features in patients with Hashimoto's thyroiditis.

Abstract Source:

Lipids Health Dis. 2018 Mar 27 ;17(1):59. Epub 2018 Mar 27. PMID: 29587770

Abstract Author(s):

Mahdieh Abbasalizad Farhangi, Parvin Dehghan, Siroos Tajmiri

Article Affiliation:

Mahdieh Abbasalizad Farhangi

Abstract:

BACKGROUND: Hashimoto's thyroiditis is associated with serious alterations in serum lipids and glucose homeostasis. The aims of the current study were to evaluate the effect of powdered Nigella sativa on serum lipids, glucose homeostasis and anthropometric variables in patients with Hashimoto's thyroiditis.

METHODS: Forty patients with Hashimoto's thyroiditis, aged between 22 and 50 years old, participated in the trial and were randomly allocated into two groups of intervention and control receiving powdered Nigella sativa or placebo daily for 8 weeks. Serum lipids, glucose homeostasis, and anthropometric variables were evaluated at baseline and after intervention.

RESULTS: Treatment with Nigella sativa significantly reduced body weight and body mass index (BMI). Serum concentrations of low density lipoprotein cholesterol (LDL) and triglyceride (TG) also decreased in Nigella sativa-treated group after 8 weeks; while serum high density lipoprotein cholesterol (HDL) significantly increased after treatment with Nigella sativa (P < 0.05). None of these changes had been observed in placebo treated group. Serum Nesfatin-1 concentrations was in inverse relationship with serum triglyceride (TG) (r = - 0.31, P = 0.04).

CONCLUSIONS: Giving attention to the potent beneficial effects of powdered black cumin seeds in improving serum lipid profile and anthropometric features in patients with Hashimoto's thyroiditis, this medicinal plant could be considered as a beneficial herbal supplement alongside with the disease- specific medications including Levothyroxine in management of Hashimoto's thyroiditis- related metabolic abnormalities.

TRIAL REGISTRATION: Iranian registry of clinical trials (registration number IRCT2014090819082N2 - Registered 2014-09-29).

Article Published Date : Mar 26, 2018

Study Type : Human Study

[14] Daily intake of N. sativa oil for two months improves abnormal semen quality in infertile men without any adverse effects

Abstract Title:

Effects of Nigella sativa L. seed oil on abnormal semen quality in infertile men: a randomized, double-blind, placebo-controlled clinical trial.

Abstract Source:

Phytomedicine. 2014 May 15 ;21(6):901-5. Epub 2014 Mar 25. PMID: 24680621

Abstract Author(s):

M Kolahdooz, S Nasri, S Zadeh Modarres, S Kianbakht, H Fallah Huseini

Article Affiliation:

M Kolahdooz

Abstract:

In recent years, wide utilization of herbal drugs has encouraged scientists to determine their impressive effects on health. Since Nigella sativa L. seed (N. sativa) has many uses including infertility in traditional medicine, the effects of Nigella sativa L. seed oil on abnormal semen quality in infertile men with abnormal semen quality are of interest. This study was conducted on Iranian infertile men with inclusion criteria of abnormal sperm morphology less than 30% or sperm counts below 20×10(6)/ml or type A and B motility less than 25% and 50% respectively. The patients in N. sativa oil group (n=34) received 2.5mlN. sativa oil and placebo group (n=34) received 2.5ml liquid paraffin two times a day orally for 2 months. At baseline and after 2 months, the sperm count, motility and morphology and semen volume, pH and round cells as primary outcomes were determined in both groups. Results showed that sperm count, motility and morphology and semen volume, pH and round cells were improved significantly in N. sativa oil treated group compared with placebo group after 2 months. It isconcluded that daily intake of 5ml N. sativa oil for two months improves abnormal semen quality in infertile men without any adverse effects.

Article Published Date : May 14, 2014

Study Type : Human Study

[15] Dosin eradicated H. pylori in about 57% of infected patients

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Abstract Title:

Combination of Nigella sativa and Honey in Eradication of Gastric Helicobacter pylori Infection.

Abstract Source:

Iran Red Crescent Med J. 2016 Nov ;18(11):e23771. Epub 2016 Jun 21. PMID: 28191328

Abstract Author(s):

Fataneh Hashem-Dabaghian, Shahram Agah, Maryam Taghavi-Shirazi, Ali Ghobadi

Article Affiliation:

Fataneh Hashem-Dabaghian

Abstract:

BACKGROUND: Gastric Helicobacter pylori is extremely common worldwide.

OBJECTIVES: The aim of this study was to assess the effectiveness of combination of Nigella sativa and honey (Dosin) in eradication of gastric H. pylori infection.

PATIENTS AND METHODS: Nineteen patients who had positive result for H. pylori infection by urea breath test (UBT) without a past history of peptic ulcer, gastric cancer or gastrointestinal bleeding, were suggested to receive one teaspoon of the mixture of Dosin (6 g/day of N. sativa as ground seeds and 12 g/day of honey) three times a day after meals for two weeks. The second UBT was used to detect the presence of H. pylori four weeks after completion of the test. In addition, symptoms of dyspepsia were scored before and after the study and analyzed with Wilcoxon signed-rank test.

RESULTS: Fourteen patients completed the study. Negative UBT was observed in 57.1% (8/14) of participants after intervention. The median and interquartile range (IQR) of total dyspepsia symptoms was significantly reduced from 5.5 (5 - 12) to 1 (0 - 4) (P = 0.005). All the patients tolerated Dosin except for one who was excluded due to mild diarrhea. No serious adverse events were reported.

CONCLUSIONS: Dosin was concluded to be an anti H. pylori and an anti-dyspeptic agent. Further studies are recommended to investigate the effect of Dosin plus antibiotics (concurrently or following another) on gastric H. pylori infection.

Article Published Date : Oct 31, 2016

Study Type : Human Study

[16] It is recommended that clinicians and wound care nurses use aloe vera gel instead of Nigella sativa oil gel for the topical treatment of diabetic ulcers

Abstract Title:

A Comparative Study of the Effects of Nigella sativa Oil Gel and Aloe Vera Gel on Wound Healing in Diabetic Rats.

Abstract Source:

J Evid Based Integr Med. 2018 Jan-Dec;23:2515690X18772804. PMID: 29717629

Abstract Author(s):

Yunita Sari, Iwan Purnawan, Dhadhang Wahyu Kurniawan, Eman Sutrisna

Article Affiliation:

Yunita Sari

Abstract:

Clinicians and wound care nurses in Indonesia usually use Nigella sativa oil (NSO) gel and aloe vera (AV) gel to treat diabetic ulcers. However, there are no studies directly comparing the effects of NSO and AV gels on wound healing, so it is unknown which of these 2 plants is better at promoting wound healing in diabetic ulcers. If the comparative efficacy between these 2 gels was known, it would be important evidence favoring the clinical use of one or the other product in Indonesia. The aim of this study was to investigate and compare the effectiveness of NSO and AV gels on wound healing in a rat model of diabetic ulcers. This experimental study involved 3 groups: NSO gel, AV gel, and controls. Our study showed that from day 5 onward, necrotic tissue and inflammation decreased in the AV gel group compared with the other groups. The wound areas on days 6 ( P = .020) and 7 ( P = .021) were significantly smaller in the AV gel group than in the NSO gel group. Reepithelialization was also better in the AV gel group than in the other groups. This is the first study to compare the effects of AV and NSO gels on wound healing in diabetic ulcers. Our study indicates that the AV gel is better than the NSO gel. Therefore, it is recommended that clinicians and wound care nurses use AV gel instead of NSO gel for the topical treatment of diabetic ulcers.

Article Published Date : Dec 31, 2017

Study Type : Human Study

[17] N sativa supplementation with inhaled maintenance therapy improves some measures of pulmonary function and inflammation in partly controlled asthma

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Abstract Title:

Effect of Nigella sativa supplementation on lung function and inflammatory mediatorsin partly controlled asthma: a randomized controlled trial.

Abstract Source:

Ann Saudi Med. 2017 Jan-Feb;37(1):64-71. PMID: 28151459

Abstract Author(s):

Ayad Mohammed Salem, Abdullah Omar Bamosa, Hatem Othman Qutub, Rakesh Kumar Gupta, Ahmed Badar, Abdelsalam Elnour, Muhammad Nasir Afzal

Article Affiliation:

Ayad Mohammed Salem

Abstract:

BACKGROUND: Nigella sativa and its derivatives have been reported to have anti-inflammatory and bronchodilator effects, but the effects have been evaluated in only a few clinical studies.

OBJECTIVES: To determine the effect of N sativa supplementation on inflammation of the airways and limitation of airflow in partly controlled asthma patients.

DESIGN: Single-blind, placebo-controlled, randomized study.

SETTING: Asthma and allergy clinic of a university hospital in eastern Saudi Arabia.

PATIENTS AND METHODS: Patients were divided into three groups. A control group (n=24) received the placebo, while NS-1 and NS-2 groups (n=26 each) received 1 and 2 g/day of N sativa, respectively, for 3 months along with maintenance inhaled therapy.

MAIN OUTCOME MEASURE(S): Asthma control test (ACT) score, fractional exhaled nitric oxide (FeNO), peak expiratory flow (PEF) variability and other pulmonary function tests, IgE, serum cytokines, and frequency of exacerbations.

RESULTS: FEF25-75% and FEV1 (% predicted) increased significantly (P<.05) at both 6 and 12 weeks in the NS-2 group. PEF variability significantly improved in both NS-1 and NS-2 groups at 6 and 12 weeks as compared with the controls (P<.05). FeNO and serum IgE decreased significantly after 12 weeks in both the NS-1 and NS-2 groups vs baseline (P<.05). Both doses of N sativa produced a significant increase in the serum IFN-gamma at 12 weeks vs baseline (P<.05) as well as a significant improvement in the ACT score at 6 and 12 weeks vs baseline (P<.001,<.01). Significantly fewer patients had exacerbations in the NS-1 group (P<.05).

CONCLUSION: N sativa supplementation with inhaled maintenance therapy improves some measures of pulmonary function and inflammation in partly controlled asthma.

LIMITATIONS: No bronchoalveolar lavage or sputum samples taken for measurement of asthma markers. ISRCTN registry: ISRCTN48853858 DOI 10.1186/ISRCTN48853858.

Article Published Date : Dec 31, 2016

Study Type : Human Study

[18] N. sativa administration in patients with HCV was tolerable, safe, decreased viral load, and improved oxidative stress, clinical condition and glycemic control in diabetic patients

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Abstract Title:

Effects of Nigella sativa on outcome of hepatitis C in Egypt.

Abstract Source:

Abstract Author(s):

Eman Mahmoud Fathy Barakat, Lamia Mohamed El Wakeel, Radwa Samir Hagag

Article Affiliation:

Eman Mahmoud Fathy Barakat, Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.

Abstract:

AIM: To evaluate the safety, efficacy and tolerability of Nigella sativa (N. sativa) in patients with hepatitis C not eligible for interferon (IFN)-α.

METHODS: Thirty patients with hepatitis C virus (HCV) infection, who were not eligible for IFN/ribavirin therapy, were included in the present study. Inclusion criteria included: patients with HCV with or without cirrhosis, who had a contraindication to IFN-α therapy, or had refused or had a financial constraint to IFN-α therapy. Exclusion criteria included: patients on IFN-α therapy, infection with hepatitis B or hepatitis I virus, hepatocellular carcinoma, other malignancies, major severe illness, or treatment non-compliance. Various parameters, including clinical parameters, complete blood count, liver function, renal function, plasma glucose, total antioxidant capacity (TAC), and polymerase chain reaction, were all assessed at baseline and at the end of the study. Clinical assessment included: hepato and/or splenomegaly, jaundice, palmar erythema, flapping tremors, spider naevi, lower-limb edema, and ascites. N. sativa was administered for three successive months at a dose of (450 mg three times daily). Clinical response and incidence of adverse drug reactions were assessed initially, periodically, and at the end of the study.

RESULTS: N. sativa administration significantly improved HCV viral load (380808.7± 610937 vs 147028.2 ± 475225.6, P = 0.001) and TAC (1.35 ± 0.5 vs 1.612 ± 0.56, P = 0.001). After N. sativa administration, the following laboratory parameters improved: total protein (7.1 ± 0.7 vs 7.5 ± 0.8, P = 0.001), albumin (3.5 ± 0.87 vs 3.69 ± 0.91, P = 0.008), red blood cell count (4.13 ± 0.9 vs 4.3 ± 0.9, P = 0.001), and platelet count (167.7 ± 91.2 vs 198.5 ± 103, P = 0.004). Fasting blood glucose (104.03 ± 43.42 vs 92.1 ± 31.34, P = 0.001) and postprandial blood glucose (143.67 ± 72.56 vs 112.1 ± 42.9, P = 0.001) were significantly decreased in both diabetic and non-diabetic HCV patients. Patients with lower-limb edema decreased significantly from baseline compared with after treatment [16 (53.30%) vs 7 (23.30%), P = 0.004]. Adverse drug reactions were unremarkable except for a few cases of epigastric pain and hypoglycemia that did not affect patient compliance.

CONCLUSION: N. sativa administration in patients with HCV was tolerable, safe, decreased viral load, and improved oxidative stress, clinical condition and glycemic control in diabetic patients.

Article Published Date : Apr 27, 2013

Study Type : Human Study

[19] N. sativa oil was found to be effective as an add-on therapy in patients of insulin resistance syndrome

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Abstract Title:

Effect of Nigella sativa oil on various clinical and biochemical parameters of insulin resistance syndrome.

Abstract Source:

Int J Diabetes Dev Ctries. 2008 Jan ;28(1):11-4. PMID: 19902033

Abstract Author(s):

Ahmad Najmi, Mohammad Nasiruddin, Rahat Ali Khan, Shahzad F Haque

Article Affiliation:

Ahmad Najmi

Abstract:

AIM: The seeds of the Nigella sativa plant have been used to promote health and fight disease for centuries, especially in the Middle East and in Southeast Asia. This plant has been a focus of much research. This clinical study was undertaken to know the adjuvant effect of N. sativa oil on various clinical and biochemical parameters of the insulin resistance syndrome.

MATERIALS AND METHODS: This prospective study was conducted at a tertiary health care center in North India. After confirmation of diagnosis, 60 patients who fulfilled the inclusion and exclusion criteria were enrolled in this study. Written informed consent was taken from all the patients enrolled. Approval from the institutional ethical committee was also obtained. The patients were divided into two groups of 30 each. In group I (the standard group), patients were advised tablet atorvastatin 10 mg once a day and tablet metformin 500 mg twice a day for a period of 6 weeks. In group II (the N. sativa group), the patients were advised tablet atorvastatin 10 mg once a day, tablet metformin 500 mg twice a day, and N. sativa oil 2.5 ml twice daily for a period of 6 weeks. Fasting and postprandial blood glucose, fasting lipid profile, and waist circumference were recorded before therapy and after completion of therapy.

RESULT: The treatment group showed significant (P<0.05) improvement with reference to total cholesterol, low density lipoprotein cholesterol (LDL-C), and fasting blood glucose (P<0.05).

CONCLUSION: N. sativa oil was found to be effective as an add-on therapy in patients of insulin resistance syndrome. N. sativa oil has a significant activity in diabetic and dyslipidemic patients.

Article Published Date : Dec 31, 2007

Study Type : Human Study

[20] N. sativa seed oil has clinical effectiveness comparable to topical diclofenac in the treatment of cyclic mastalgia

Abstract Title:

Effectiveness of Topical Nigella sativa Seed Oil in the Treatment of Cyclic Mastalgia: A Randomized, Triple-Blind, Active, and Placebo-Controlled Clinical Trial.

Abstract Source:

Planta Med. 2015 Nov 19. Epub 2015 Nov 19. PMID: 26584456

Abstract Author(s):

Hasan Fallah Huseini, Saeed Kianbakht, Mohammad Hossein Mirshamsi, Ali Babaei Zarch

Article Affiliation:

Hasan Fallah Huseini

Abstract:

Cyclic mastalgia is common in women and has no optimal therapy. Analgesic effects of Nigella sativa have been reported. Thus, the effect of a standardized N. sativa seed oil (600 mg applied to the site of pain bis in die for 2 months) on the 10-centimeter visual analog scale scores of pain severity in 52 women with cyclic mastalgia was compared to that of topical diclofenac (20 mg bis in die) (n = 51) and placebo (n = 53). There was no significant difference between the 1- and 2-month pain scores in the active treatment groups (p > 0.05). The pain scores of the active treatment groups did not differ significantly at 1 and 2 months (p > 0.05). The endpoint pain scores of the active treatment groups decreased significantly compared with the baseline (both p < 0.001). The pain scores of the active treatment groups at 1 and 2 months were significantly smaller than those of the placebo group (both p < 0.001). The pain scores of the placebo group at 1 and 2 months were not significantly different from the baseline (p > 0.05). No adverse effect was observed. In conclusion, topical N. sativa seed oil is safe, more effective than placebo, and has clinical effectiveness comparable to topical diclofenac in the treatment of cyclic mastalgia.

Article Published Date : Nov 18, 2015

Study Type : Human Study

[21] N. sativa seed supplementation during specific immunotherapy of allergic rhinitis may be considered a potential adjuvant therapy

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Abstract Title:

Potential adjuvant effects of Nigella sativa seeds to improve specific immunotherapy in allergic rhinitis patients.

Abstract Source:

Med Princ Pract. 2010 ;19(3):206-11. Epub 2010 Mar 29. PMID: 20357504

Abstract Author(s):

Hülya Işik, Adile Cevikbaş, Umran Soyoğul Gürer, Bayram Kiran, Yağiz Uresin, Pervin Rayaman, Erkan Rayaman, Burçak Gürbüz, Suna Büyüköztürk

Article Affiliation:

Hülya Işik

Abstract:

OBJECTIVE: To investigate the effects of Nigella sativa seed supplementation on symptom levels, polymorphonuclear leukocyte (PMN) functions, lymphocyte subsets and hematological parameters of allergic rhinitis.

SUBJECTS AND METHODS: Twenty-four patients randomly selected from an experimental group of 31 (mean age 34 years) sensitive to house dust mites with allergic rhinitis and a control group of 8 healthy volunteers (mean age 23 years) were treated with allergen-specific immunotherapy in conventional doses for 30 days. After a month of immunotherapy, 12 of the 24 patients and the 8 healthy volunteers were given N. sativa seed supplementation (2 g/day orally) for 30 days. The remaining 12 patients continued only on immunotherapy during the same period. The other 7 patients were given 0.1 ml saline solution subcutaneously once a week as a placebo. The symptom scores, PMN functions, lymphocyte subsets and other hematological parameters were evaluated before and after all treatment periods.

RESULTS: There was a statistically significant increase in the phagocytic and intracellular killing activities of PMNs of patients receiving specific immunotherapy, especially after the addition of N. sativa seed. The CD8 counts of patients receiving specific immunotherapy plus N. sativa seed supplementation significantly increased compared to patients receiving only specific immunotherapy. PMN functions of healthy volunteers significantly increased after N. sativa seed supplementation compared to baseline.

CONCLUSION: N. sativa seed supplementation during specific immunotherapy of allergic rhinitis may be considered a potential adjuvant therapy.

Article Published Date : Dec 31, 2009

Study Type : Human Study

[22] N. sativa seeds is beneficial in relieving the symptoms of knee osteoarthritis

Abstract Title:

Effect of an orally formulated processed black cumin, from Iranian traditional medicine pharmacopoeia, in relieving symptoms of knee osteoarthritis: A prospective, randomized, double-blind and placebo-controlled clinical trial.

Abstract Source:

Int J Rheum Dis. 2017 Apr 5. Epub 2017 Apr 5. PMID: 28378356

Abstract Author(s):

Ahmad Salimzadeh, Anahita Ghourchian, Rasool Choopani, Homa Hajimehdipoor, Mohammad Kamalinejad, Maryam Abolhasani

Article Affiliation:

Ahmad Salimzadeh

Abstract:

AIM: Osteoarthritis is a global health problem, especially for the elderly. A good replacement for non-surgical treatments is the use of traditional medicines. We selected a revere plant (Nigella sativa L.), a widely utilized medicinal herb for the treatment of inflammatory conditions, from the Iranian traditional medicine (ITM) pharmacopoeia with proven anti-inflammatory and analgesic actions.

METHODS: We performed a prospective, randomized, double-blind, and placebo-controlled clinical trial, in order to investigate whether the herb is useful in alleviating the symptoms of knee osteoarthritis. American College of Rheumatology clinical criteria were the basis of diagnosis, while the Knee injury and Osteoarthritis Outcome Score (KOOS) questionnaire was considered as the main outcome measure. One hundred and ten eligible patients were assigned to receive a placebo or an active intervention (2 g/day of processed N. sativa seed powder in divided doses). Acetaminophen tablets were the rescue medicine. Finally, 40 patients in the placebo group and 37 patients in the active group completed the trial and were included in the statistical analysis.

RESULTS: Both cohorts demonstrated statistically significant within-group differences (P<0.05) in some subscales that were more prominent in the active group without any considerable adverse effects. Nevertheless, KOOS score results and the mean number of acetaminophen tablets used by patients showed no statistically significant between-group differences.

CONCLUSIONS: It can be concluded that future programmed studies with larger sample sizes, longer follow-up periods, and other forms of N. sativa seeds as an active intervention is necessary to evaluate its efficacy in relieving the symptoms of knee osteoarthritis.

Article Published Date : Apr 04, 2017

Study Type : Human Study

[23] Nigella sativa (black cumin) has potent antiasthmatic properties

Abstract Title:

Antiasthmatic effect of Nigella sativa in airways of asthmatic patients.

Abstract Source:

Phytomedicine. 2010 Feb 8. Epub 2010 Feb 8. PMID: 20149611

Abstract Author(s):

M H Boskabady, N Mohsenpoor, L Takaloo

Article Affiliation:

Department of Physiology, Medical School and Pharmacology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract:

In the present study, the antiasthmatic (bronchodilatory) effect of the boiled extract of Nigella sativa in the airways of asthmatic patients was examined. The bronchodilatory effects of 50 and 100mg/kg of boiled extract in comparison with 6mg/kg theophylline were studied on 15 asthmatic patients. Pulmonary function tests (PFTs) including forced expiratory volume in one second (FEV(1)), peak expiratory flow (PEF), maximal mid expiratory flow (MMEF), maximal expiratory flow at 75, 50 and 25% of the FVC (MEF(75), MEF(50), and MEF(25,) respectively) and specific airway conductance (sGaw) were measured before administration and repeated , 30, 60, 90 120, 150, and 180min after administration of the oral extract and theophylline. The results showed that the extract caused significant increases in all measured pulmonary function tests (PFTs), in most time intervals, (p<0.05 to p<0.001). However, the increase in FEV(1), MMEF and MEF(50) due to both doses of boiled extract and increase in MEF(75) and MEF(25) due to its lower doses were significantly lower than those of theophylline (p<0.05 to p<0.001). The onset of brochodilatory effect of extract was similar to that of theophylline beginning 30min, and the effect of extract decline after 150min following administration similar to the effect of theophylline. The effect of both doses of the extract was also significantly less than that of salbutamol at 30 minutes post administration (p<0.001 for all cases). The results of the present study showed that Nigella sativa has a relatively potent antiasthmatic effect on asthmatic airways. However, the effects of boiled extract of this plant on most measured PFTs was less than those of theophylline at concentrations used.

Article Published Date : Feb 08, 2010

Study Type : Human Study

[24] Nigella sativa could improve inflammation and reduce oxidative stress in patients with rheumatoid arthritis

Abstract Title:

Effects of Nigella sativa oil extract on inflammatory cytokine response and oxidative stress status in patients with rheumatoid arthritis: a randomized, double-blind, placebo-controlled clinical trial.

Abstract Source:

Avicenna J Phytomed. 2016 Jan-Feb;6(1):34-43. PMID: 27247920

Abstract Author(s):

Vahid Hadi, Sorayya Kheirouri, Mohammad Alizadeh, Alireza Khabbazi, Hossein Hosseini

Article Affiliation:

Vahid Hadi

Abstract:

OBJECTIVE: Nigella sativa is a medicinal plant that has long been used in traditional medicine for treating various conditions. Numerous animal studies provided evidences that the seed may elicit a broad anti-inflammatory/anti-oxidant activity. The aim of the present clinical trial was to evaluate anti-inflammatory and antioxidant properties of Nigella sativa oil in patients with rheumatoid arthritis (RA).

MATERIALS AND METHODS: Forty-two patients with RA were assigned into two groups in this randomized, double blind, placebo-controlled clinical trial. Subjects in intervention group received two capsules, 500 mg each, of Nigella sativa oil, each day for 8 weeks. The other group consumed two capsules as placebo per day for the same period of time. Serum TNF-α, IL-10, and whole blood levels of oxidative stress parameters were measured at baseline and end of the trial.

RESULTS: The serum level of IL-10 was increased in the Nigella sativa group (p<0.01). Moreover, treatment with Nigella sativa led to significant reduction of serum MDA and NO compared with baseline (p<0.05). There were no significant differences in the TNF-α, SOD, catalase, and TAS values between or within the groups, before and after the intervention (p>0.05).

CONCLUSION: This study indicates that Nigella sativa could improve inflammation and reduce oxidative stress in patients with RA. It is suggested that Nigella sativa may be a beneficial adjunct therapy in this population of patients.

Article Published Date : Dec 31, 2015

Study Type : Human Study

[25] Nigella sativa enhances memory, attention and cognition in healthy human volunteers

Abstract Title:

The effect of Nigella sativa Linn. seed on memory, attention and cognition in healthy human volunteers.

Abstract Source:

J Ethnopharmacol. 2013 Jul 30 ;148(3):780-6. Epub 2013 May 21. PMID: 23707331

Abstract Author(s):

Muhammad Shahdaat Bin Sayeed, Md Asaduzzaman, Helal Morshed, Md Monir Hossain, Mohammad Fahim Kadir, Md Rezowanur Rahman

Article Affiliation:

Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Bangladesh; Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka, Bangladesh. Electronic address: shahdaat2013@yahoo.com.

Abstract:

BACKGROUND: Experimental evidences have demonstrated that Nigella sativa Linn. seed (NS) has positive modulation effects on aged rats with memory impairments, prevents against hippocampal pyramidal cell loss and enhances consolidation of recall capability of stored information and spatial memory in rats. NS has neuroprotective, nephroprotective, lung protective, cardioprotective, hepatoprotective activities as established by previous studies on animals. Several clinical trials with NS on human have also demonstrated beneficial effect.

AIM OF THE STUDY: The present study was designed to investigate the effects of NS on memory, attention and cognition in healthy elderly volunteers. Furthermore, safety profile of NS was assessed during the nine-week study period.

METHODS: Forty elderly volunteers were recruited and divided randomly into group A and group B-each consisting of 20 volunteers. The treatment procedure for group A was 500mg NS capsule twice daily for nine weeks and Group B received placebo instead of NS in the similar manner. All the volunteers were assessed for neuropsychological state and safety profile twice before treatment and after nine weeks. The neuropsychological tests were logical memory test, digit span test, Rey-Osterrieth complex figure test, letter cancellation test, trail making test and stroop test. Safety profile was assessed by measuring biochemical markers of Cardiac (total cholesterol, triglycerides and high density lipoprotein cholesterol, very low density lipoprotein, low density lipoprotein cholesterol, creatine kinase-MB); Liver (aspartate aminotransferase, alanin aminotransferase, alkaline phosphatase, total protein, albumin, bilirubin) and Kidney (creatinine and blood urea nitrogen) through using commercial kits.

RESULTS: There was significant difference (p<0.05) in the score of logical memory test-I and II, total score of digit span, 30min delayed-recall, percent score in Rey-Osterrieth complex figure test, time taken to complete letter cancellation test, time taken in trail making test-A and test-B, score in part C of stroop test due to ingestion of NS for nine weeks. There were not statistically significant changes (p>0.05) in any of the biochemical markers of cardiac, liver, kidney function during this nine-week study period.

CONCLUSIONS: The current study demonstrates the role of NS in enhancing memory, attention and cognition. Therefore, whether NS could be considered as potential food supplement for preventing or slow progressing of Alzheimer disease needs further investigations. However, study with Alzheimer's patients with large population size for longer period of time is recommended before using NS daily and extensive phytochemical investigations are recommended for novel drug discovery from NS for treating cognitive disorders.

Article Published Date : Jul 29, 2013

Study Type : Human Study

[26] Nigella sativa oil concurrent with a low-calorie diet decreased weight and increased SOD levels in obese women

Abstract Title:

Oxidative Stress Responses to Nigella sativa Oil Concurrent with a Low-Calorie Diet in Obese Women: A Randomized, Double-Blind Controlled Clinical Trial.

Abstract Source:

Phytother Res. 2015 Jul 14. Epub 2015 Jul 14. PMID: 26179113

Abstract Author(s):

Nazli Namazi, Reza Mahdavi, Mohammad Alizadeh, Safar Farajnia

Article Affiliation:

Nazli Namazi

Abstract:

The aim of the present study was to determine the effects of Nigella sativa (NS) oil concurrent with a low-calorie diet on lipid peroxidation and oxidative status in obese women. In this double-blind placebo-controlled randomized clinical trial, 50 volunteer obese (body mass index = 30-35 kg/m(2) ) women aged 25-50 years old were recruited. Participants were randomly divided into intervention (n = 25) and placebo (n = 25) groups. They received a low-calorie diet with 3 g/day NS oil or low-calorie diet with 3 g/day placebo for 8 weeks. Forty-nine women (intervention group = 25; placebo group = 24) completed the trial. NS oil concurrent with a low-calorie diet decreased weight in the NS group compared to the placebo group (-4.80 ± 1.50 vs. -1.40 ± 1.90 kg; p < 0.01). Comparison of red blood cell superoxidase dismutase (SOD) indicated significant changes in the NS group compared to the placebo group at the end of the study (88.98 ± 87.46 vs. -3.30 ± 109.80 U/gHb; p < 0.01). But no significant changes in lipid peroxidation, glutathione peroxidase, and total antioxidant capacity concentrations were observed. NS oil concurrent with a low-calorie diet decreased weight and increased SOD levels in obese women. However, more studies are suggested to confirm the positive effects of NS in obesity and its complications. Copyright © 2015 John Wiley&Sons, Ltd.

Article Published Date : Jul 13, 2015

Study Type : Human Study

[27] Nigella sativa oil supplementation combined with a calorie-restricted diet may modulate systemic inflammatory biomarkers in obese women

Abstract Title:

Nigella sativa oil with a calorie-restricted diet can improve biomarkers of systemic inflammation in obese women: A randomized double-blind, placebo-controlled clinical trial.

Abstract Source:

J Clin Lipidol. 2016 Sep-Oct;10(5):1203-11. Epub 2015 Dec 7. PMID: 27678438

Abstract Author(s):

Reza Mahdavi, Nazli Namazi, Mohammad Alizadeh, Safar Farajnia

Article Affiliation:

Reza Mahdavi

Abstract:

BACKGROUND: Inflammation is one of the primary mechanisms in the development of metabolic complications. Although anti-inflammatory characteristics of Nigella sativa (NS) have been indicated in animal models, clinical trials related to the effects of NS on inflammatory parameters are relatively scarce.

OBJECTIVE: The aim of the present study was to determine the effects of NS oil combined with a calorie-restricted diet on systemic inflammatory biomarkers in obese women.

METHODS: In this double-blind placebo-controlled randomized clinical trial, 90 volunteer obese (body mass index = 30-34.9 kg/m(2)) women aged 25-50 years were recruited. Participants were randomly divided into two groups, an intervention group (n = 45) and a placebo group (n = 45). Each group received either: (1) a low-calorie diet with 3 g/day of NS oil or (2) a low-calorie diet with 3 g/day placebo for 8 weeks.

RESULTS: A total of 84 females (intervention group = 43; placebo group = 41) completed the trial. Subjects in the intervention group did not report any side effects with the NS oil supplementation. NS oil decreased serum levels of tumor necrosis factor-alpha (-40.8% vs -16.1%, P = .04) and high-sensitivity C-reactive protein (-54.5% vs -21.4%, P = .01) compared to the placebo group. However, there were no significant changes in interleukin-6 levels (-8.6 vs -2.4%, P = .6) in the NS group compared to the placebo group.

CONCLUSIONS: NS oil supplementation combined with a calorie-restricted diet may modulate systemic inflammatory biomarkers in obese women. However, more studies are needed to clarify the efficacy of NS oil as an adjunct therapy to improve inflammatory parameters in obese subjects.

Article Published Date : Aug 31, 2016

Study Type : Human Study

[28] Nigella sativa oil supplementation had a protective effect in diabetic nephropathy

Abstract Title:

Protective role of Nigella sativa in diabetic nephropathy: A randomized clinical trial.

Abstract Source:

Saudi J Kidney Dis Transpl. 2017 Jan-Feb;28(1):9-14. PMID: 28098097

Abstract Author(s):

Zafar Masood Ansari, Mohammad Nasiruddin, Rahat Ali Khan, Shahzad Faizul Haque

Article Affiliation:

Zafar Masood Ansari

Abstract:

This study aims to evaluate efficacy and safety of Nigella sativa oil supplementation in patients with chronic kidney disease Stage 3 and 4 due to diabetic nephropathy. It was a prospective, comparative, and open-label study. Patients were randomized into two groups. Group 1 (Control) received conservative management of diabetic nephropathy, whereas Group 2 (Test) received N. sativa oil (2.5 mL, once daily and per orally) along with conservative management for 12 weeks. Blood glucose, hemogram, and kidney function test were done at 0, 6, and 12 weeks of treatment. Significance of differences between pre- and post-treatment values in each group was assessed using Student's paired t-test and between the groups using unpaired t-test. We found a drop in blood glucose, serum creatinine, blood urea, and 24 h total urinary protein levels and a rise in glomerular filtration rate, 24 h total urinary volume, and hemoglobin level in the treatment group compared to the control group.

Article Published Date : Dec 31, 2016

Study Type : Human Study

[29] Nigella sativa seeds showed a decrease in incidence of febrile neutropenia in children with brain tumors undergoing chemotherapy

Abstract Title:

Effect of Nigella sativa seed administration on prevention of febrile neutropenia during chemotherapy among children with brain tumors.

Abstract Source:

Childs Nerv Syst. 2017 Mar 27. Epub 2017 Mar 27. PMID: 28349493

Abstract Author(s):

HebatAlla Fathi Mohamed Mousa, Nesrin Kamal Abd-El-Fatah, Olfat Abdel-Hamid Darwish, Shehata Farag Shehata, Shady Hassan Fadel

Article Affiliation:

HebatAlla Fathi Mohamed Mousa

Abstract:

PURPOSE: Seeds of Nigella sativa (NS) are used to combat various disease conditions through their antibacterial effects. To evaluate the seeds' potential, we studied their effect on the prevention of febrile neutropenia (FN) in children with brain tumors.

METHODS: A randomized pretest-post-test control group study including 80 children (2-18 years) with brain tumors undergoing chemotherapy were equally allocated into two groups. Intervention group received 5 g of NS seeds daily throughout treatment while controls received nothing. CBC with differentials, incidence of FN, and LOS were noted on each follow-up.

RESULTS: The majority of children 38/40 (95%), of the intervention group, took the seeds for 3-9 consecutive months. Eight out of 372 (2.2%) FN episodes were experienced by children of intervention group compared to controls 63/327 (19.3%) (p = 0.001) and a shorter LOS (median = 2.5 days) vs 5 days in the control group (p = 0.006). Children in both groups belonged to almost same geographical area with similar socio-economic background. Weights of children were almost equal at diagnosis.

CONCLUSION: NS seeds showed a decrease in incidence of FN in children with brain tumors with shortening of subsequent LOS which may improve their outcome and thereby quality of life. Larger scale studies are needed to further evaluate the seeds' potential.

Article Published Date : Mar 26, 2017

Study Type : Human Study

[30] Nigella sativa Supplementation Improves Asthma Control and Biomarkers: A Randomized, Double-Blind, Placebo-Controlled Trial

Abstract Title:

Nigella sativa Supplementation Improves Asthma Control and Biomarkers: A Randomized, Double-Blind, Placebo-Controlled Trial.

Abstract Source:

Phytother Res. 2017 Jan 17. Epub 2017 Jan 17. PMID: 28093815

Abstract Author(s):

Abdulrahman Koshak, Li Wei, Emad Koshak, Siraj Wali, Omer Alamoudi, Abdulrahman Demerdash, Majdy Qutub, Peter Natesan Pushparaj, Michael Heinrich

Article Affiliation:

Abdulrahman Koshak

Abstract:

Poor compliance with conventional asthma medications remains a major problem in achieving asthma control. Nigella sativa oil (NSO) is used traditionally for many inflammatory conditions such as asthma. We aimed to investigate the benefits of NSO supplementation on clinical and inflammatory parameters of asthma. NSO capsules 500 mg twice daily for 4 weeks were used as a supplementary treatment in a randomized, double-blind, placebo-controlled trial in asthmatics (clinicaltrials.gov: NCT02407262). The primary outcome was Asthma Control Test score. The secondary outcomes were pulmonary function test, blood eosinophils and total serum Immunoglobulin E. Between 1 June and 30 December 2015, 80 asthmatics were enrolled, with 40 patients in each treatment and placebo groups. After 4 weeks, ten patients had withdrawn from each group. Compared with placebo, NSO group showed a significant improvement in mean Asthma Control Test score 21.1 (standard deviation = 2.6) versus 19.6 (standard deviation = 3.7) (p = 0.044) and a significant reduction in blood eosinophils by -50 (-155 to -1) versus 15 (-60 to 87) cells/μL (p = 0.013). NSO improved forced expiratory volume in 1 second as percentage of predicted value by 4 (-1.25 to 8.75) versus 1 (-2 to 5) but non-significant (p = 0.170). This randomized, double-blind, placebo-controlled trial demonstrated that NSO supplementation improves asthma control with a trend in pulmonary function improvement. This was associated with a remarkable normalization of blood eosinophlia. Future studies should follow asthmatics for longer periods in a multicentre trial. Copyright © 2017 John Wiley&Sons, Ltd.

Article Published Date : Jan 16, 2017

Study Type : Human Study

[31] Powdered Nigella sativa has potent beneficial effects in improving thyroid status and anthropometric variables

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Abstract Title:

The effects of Nigella sativa on thyroid function, serum Vascular Endothelial Growth Factor (VEGF) - 1, Nesfatin-1 and anthropometric features in patients with Hashimoto's thyroiditis: a randomized controlled trial.

Abstract Source:

BMC Complement Altern Med. 2016 Nov 16 ;16(1):471. Epub 2016 Nov 16. PMID: 27852303

Abstract Author(s):

Mahdieh Abbasalizad Farhangi, Parvin Dehghan, Siroos Tajmiri, Mehran Mesgari Abbasi

Article Affiliation:

Mahdieh Abbasalizad Farhangi

Abstract:

BACKGROUND: Hashimoto's thyroiditis is an autoimmune disorder and the most common cause of hypothyroidism. The use of Nigella sativa, a potent herbal medicine, continues to increase worldwide as an alternative treatment of several chronic diseases including hyperlipidemia, hypertension and type 2 diabetes mellitus (T2DM). The aim of the current study was to evaluate the effects of Nigella sativa on thyroid function, serum Vascular Endothelial Growth Factor (VEGF) - 1, Nesfatin-1 and anthropometric features in patients with Hashimoto's thyroiditis.

METHODS: Forty patients with Hashimoto's thyroiditis, aged between 22 and 50 years old, participated in the trial and were randomly allocated into two groups of intervention and control receiving powdered Nigella sativa or placebo daily for 8 weeks. Changes in anthropometric variables, dietary intakes, thyroid status, serum VEGF and Nesfatin-1 concentrations after 8 weeks were measured.

RESULTS: Treatment with Nigella sativa significantly reduced body weight and body mass index (BMI). Serum concentrations of thyroid stimulating hormone (TSH) and anti-thyroid peroxidase (anti-TPO) antibodies decreased while serum T3 concentrations increased in Nigella sativa-treated group after 8 weeks. There was a significant reduction in serum VEGF concentrations in intervention group. None of these changes had been observed in placebo treated group. In stepwise multiple regression model, changes in waist to hip ratio (WHR) and thyroid hormones were significant predictors of changes in serum VEGF and Nesgfatin-1 values in Nigella sativa treated group (P < 0.05).

CONCLUSIONS: Our data showed a potent beneficial effect of powdered Nigella sativa in improving thyroid status and anthropometric variables in patients with Hashimoto's thyroiditis. Moreover, Nigella sativa significantly reduced serum VEGF concentrations in these patients. Considering observed health- promoting effect of this medicinal plant in ameliorating the disease severity, it can be regarded as a useful therapeutic approach in management of Hashimoto's thyroiditis.

TRIAL REGISTRATION: Iranian registry of clinical trials (registration number IRCT2015021719082N4 - Registered March-15-2015).

Article Published Date : Nov 15, 2016

Study Type : Human Study

[32] Protective Effects of Nigella sativa on Metabolic Syndrome in Menopausal Women

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Abstract Title:

Protective Effects of Nigella sativa on Metabolic Syndrome in Menopausal Women.

Abstract Source:

Adv Pharm Bull. 2014 ;4(1):29-33. Epub 2013 Dec 23. PMID: 24409406

Abstract Author(s):

Ramlah Mohamad Ibrahim, Nurul Syima Hamdan, Maznah Ismail, Suraini Mohd Saini, Saiful Nizam Abd Rashid, Latiffah Abd Latiff, Rozi Mahmud

Article Affiliation:

Ramlah Mohamad Ibrahim

Abstract:

Purpose: This study was conducted in menopausal women to determine the metabolic impact of Nigella sativa. Methods: Thirty subjects who were menopausal women within the age limit of 45-60 were participated in this study and randomly allotted into two experimental groups. The treatment group was orally administered with N. sativa seeds powder in the form of capsules at a dose of 1g per day after breakfast for period of two months and compared to control group given placebo. Anthropometric and biochemical parameters were measured at baseline, 1st month, 2nd month and a month after treatment completed to determine their body weight, serum lipid profile and fasting blood glucose (FBG). Results: The treatment group showed slight reduction with no significant difference in body weight changes of the respondents. However, significant (p<0.05) improvement was observed in total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), and blood glucose (p<0.05). Conclusion: These results suggested that treatment with N. sativa exert a protective effect by improving lipid profile and blood glucose which are in higher risk to be elevated during menopausal period.

Article Published Date : Dec 31, 2013

Study Type : Human Study

[33] Supplementation of honey and N. sativa can cause significant symptomatic improvement of patients with functional dyspepsia whom received the standard anti-secretory therapy

Abstract Title:

Efficacy and safety of Honey based formulation of nigella sativa seed oil in functional dyspepsia: A double blind randomized controlled clinical trial.

Abstract Source:

J Ethnopharmacol. 2015 Sep 16. Epub 2015 Sep 16. PMID: 26386381

Abstract Author(s):

Reza Mohtashami, Hasan Fallah Huseini, Mojtaba Heydari, Mohsen Amini, Zainab Sadeqhi, Habib Ghaznavi, Saeed Mehrzadi

Article Affiliation:

Reza Mohtashami

Abstract:

ETHNOPHARMACOLOGICAL RELEVANCE: A honey based formulation from Nigella sativa L. (N. sativa) has been used in Traditional Persian Medicine for upper gastrointestinal symptoms. Considering the traditional use of this formulation and its ingredients known pharmacologic effects, this study aimed to evaluate the efficacy and safety of N. sativa seed oil mixed with honey in treatment of patients with functional dyspepsia.

METHODS AND MATERIALS: Seventy patients diagnosed with functional dyspepsia according to ROME III criteria and confirmed by upper gastrointestinal endoscopy were selected to receive a traditional honey based formulation of Nigella sativa (5mlN. sativa oil orally daily) or placebo for 8 weeks in a double-blind randomized placebo-controlled clinical trial using a parallel design with a 1:1 allocation ratio. Patients were evaluated prior to and following 8 weeks of the intervention in terms of the Hong Kong index of dyspepsia severity, presence of H. pylori infection based on urease test, scores in different domains of short form (SF-36) health survey, and any observed adverse events.

RESULTS: The mean scores of Hong Kong index of dyspepsia severity sores and the rate of H. pylori infection were significantly lower in the N. sativa group comparing the placebo group after the intervention (P<0.001). No serious adverse event was reported.

CONCLUSION: This study showed that adjuvant supplementation of honey based formulation of N. sativa can cause significant symptomatic improvement of patients with functional dyspepsia whom received the standard anti-secretory therapy. The results should be investigated further in studies with longer duration and larger sample size.

Article Published Date : Sep 15, 2015

Study Type : Human Study

[34] The combination of black seeds and turmeric can be recommended with lifestyle modification as a starting point for patients with MetS

Abstract Title:

Clinical efficacy of the co-administration of Turmeric and Black seeds (Kalongi) in metabolic syndrome - a double blind randomized controlled trial - TAK-MetS trial.

Abstract Source:

Complement Ther Med. 2015 Apr ;23(2):165-74. Epub 2015 Jan 14. PMID: 25847554

Abstract Author(s):

F Amin, N Islam, Nfn Anila, A H Gilani

Article Affiliation:

F Amin

Abstract:

OBJECTIVE: To compare the clinical efficacy of Black seeds and Turmeric alone and its co-administration in lower doses among patients with metabolic syndrome (MetS).

DESIGN: Double-blind-randomized-controlled trial.

SETTING: Hijrat colony, Karachi, Pakistan.

INTERVENTION: Apparently healthy males (n=250), who screened positive for MetS, were randomized to either Black seeds (1.5g/day), Turmeric (2.4g/day), its combination (900mg Black seeds and 1.5g Turmeric/day) or placebo for 8 weeks.

MAIN OUTCOME MEASURES: body-mass-index (BMI), body-fat-percent (BF%), waist-circumference (WC), hip-circumference (HC), blood pressure (BP), lipid-profile (cholesterol, HDL-cholesterol, LDL-cholesterol and TG), fasting blood glucose (FBG) and c-reactive protein (CRP).

RESULTS: At 4 weeks, compared to baseline, Black seed and Turmeric alone showed improvement in BMI, WC and BF%. Combination improved all parameters except HDL-cholesterol with lower FBG and LDL-cholesterol as compared to placebo. At 8 weeks, compared to placebo, Black seeds reduced lipids and FBG, while Turmeric reduced LDL-cholesterol and CRP. Interestingly, combination group with 60% dose of the individual herbs showed an improvement in all parameters from baseline. When compared to placebo, it reduced BF%, FBG, cholesterol, TG, LDL-cholesterol, CRP and raised HDL-cholesterol.

CONCLUSION: Turmeric and Black seeds showed improvement in all parameters of metabolic syndrome, when co-administered at 60% of doses of individual herbs with enhanced efficacy and negligible adverse-effects. The combination of Black seeds and Turmeric can therefore, be recommended with lifestyle modification as a starting point for patients with MetS to halt its future complications and progression.

Article Published Date : Mar 31, 2015

Study Type : Human Study

[35] The daily use of black cumin seeds for 2 months may have a blood pressure-lowering effect in patients with mild hypertension

Abstract Title:

Antihypertensive effect of Nigella sativa seed extract in patients with mild hypertension.

Abstract Source:

Braz J Med Biol Res. 2006 Apr;39(4):421-9. Epub 2006 Apr 3. PMID: 18705755

Abstract Author(s):

Farshad Roghani Dehkordi, Amir Farhad Kamkhah

Abstract:

Hypertension (HT) is a lifestyle-related disease and dietary modifications are effective for its management and prevention. We conducted a randomized, double-blind, placebo-controlled trial to evaluate the efficacy of treatment with an oral Nigella sativa (NS) seed extract supplement in patients with mild HT. Subjects were randomized into three groups: a placebo and two test groups that received 100 and 200 mg of NS extract twice a day. After 8 weeks, systolic blood pressure (SBP) values in both case groups were found to be significantly reduced when compared with the baseline values for each group. In addition, the decrease in SBP in the two case groups was statistically significant relative to the placebo group (P<0.05-0.01). Meanwhile, diastolic blood pressure (DBP) values in the case groups were found to be significantly reduced from the baseline and a significant reduction was also observed in these groups (P<0.01) when compared with the placebo group. In addition, extract administration reduced both SBP and DBP in a dose-dependent manner. Meanwhile, NS extract caused a significant decline in the level of total and low-density-lipoprotein (LDL)-cholesterol relative to baseline data. No complications caused by NS were observed. The results suggest that the daily use of NS seed extract for 2 months may have a blood pressure-lowering effect in patients with mild HT.

Article Published Date : Apr 01, 2006

Study Type : Human Study

[36] The fixed oil extracted from Nigella sativa seeds has an in vitro antisickling activity

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Abstract Title:

The effect of fixed oil and water extracts of Nigella sativa on sickle cells: an in vitro study.

Abstract Source:

Singapore Med J. 2010 Mar ;51(3):230-4. PMID: 20428745

Abstract Author(s):

N K Ibraheem, J H Ahmed, M K Hassan

Article Affiliation:

N K Ibraheem

Abstract:

INTRODUCTION: Various drugs have been investigated in the treatment of sickle cell disease (SCD), such as hydroxyurea, piracetam and calcium antagonists. Most of these drugs are potentially toxic and are not suitable for long-term therapy. Recently, Nigella sativa (NS) has been reported to have calcium antagonist and antioxidant activities, both of which play a role in the management of the disease. This study aimed to investigate the in vitro antisickling effect of extracts from NS.

METHODS: Thirty-two patients with SCD, aged 7-47 years old, were recruited for the study. A total of 3 ml of venous blood was collected from each patient and divided into six tubes with heparin. The blood was mixed with 0.5 ml of either 0.1 percent, 0.05 percent or 0.01 percent v/v of the oil extract of NS. A slide was prepared by spreading a drop of treated blood, covered with a cover slide to ensure the complete deoxygenation condition. The separation of irreversibly sickled cells (ISCs) was performed on eight patients by a density gradient (Percoll-Renografin) centrifugation method.

RESULTS: The 0.1 percent v/v concentration of the oil extract of NS resulted in an approximately 80 percent reduction in the formation of sickle cells. The 0.05 percent v/v concentration of NS produced an intermediate effect, while the 0.01 percent v/v concentration had no effect on the formation of sickle cells. The 0.1 percent v/v concentration of the fixed oil of NS led to a considerable reduction in the formation of ISCs.

CONCLUSION: The fixed oil extracted from NS seeds has an in vitro antisickling activity.

Article Published Date : Feb 28, 2010

Study Type : Human Study

[37] The water extract of black cumin seed has significant anti-epileptic activity in children

Abstract Title:

The effect of Nigella sativa L. (black cumin seed) on intractable pediatric seizures.

Abstract Source:

Med Sci Monit. 2007 Dec;13(12):CR555-9. PMID: 18049435

Abstract Author(s):

Javad Akhondian, Ali Parsa, Hassan Rakhshande

Abstract:

"BACKGROUND: Despite the availability and use of numerous antiepileptic drugs (AEDs), nearly 15% of childhood epilepsy cases are resistant to treatment. However, in traditional medicine, Nigella Sativa L. ("black cumin seed") has been known for its anticonvulsant effects. This plant is naturally distributed in Iran and has been widely used as a natural remedy for a long time. In this study the efficacy of this agent in reducing the frequency of seizures in childhood refractory epilepsy was assessed. MATERIAL/METHODS: In this double-blinded crossover clinical trial conducted on children with refractory epilepsy, the aqueous extract of black seed was administered as an adjunct therapy and the effects were compared with those of a placebo. Twenty-three children were entered in the study and 20 remained in the study (13 months to 13 years old, 10 boys and 10 girls). All patients were receiving constant treatment for at least one month before the study. They received extract (40 mg/kg/8 h) or placebo for a period of four weeks and between these periods for two weeks they received only their pre-existing anti-epileptic drugs (AEDs). RESULTS: The mean frequency of seizures decreased significantly during treatment with extract (p<0.05). CONCLUSIONS: It can be concluded that the water extract of Nigella sativa L. has antiepileptic effects in children with refractory seizures.

Article Published Date : Dec 01, 2007

Study Type : Human Study

[38] This study gives strength to the potential relevance of Nigella sativa in clinical management of rheumatoid arthritis

Abstract Title:

Immunomodulatory Effect of Nigella sativa Oil on T Lymphocytes in Patients with Rheumatoid Arthritis.

Abstract Source:

Immunol Invest. 2016 May ;45(4):271-83. Epub 2016 Apr 21. PMID: 27100726

Abstract Author(s):

Sorayya Kheirouri, Vahid Hadi, Mohammad Alizadeh

Article Affiliation:

Sorayya Kheirouri

Abstract:

BACKGROUND AND OBJECTIVES: Abundant evidence indicates the involvement of CD4(+), CD8(+), and CD4(+)CD25(+) T lymphocytes in the induction and/or protection of rheumatoid arthritis (RA). We aimed to investigate the modulatory effect of Nigella sativa (NS) oil on the selected T cell subset percentage in females with RA.

METHODS: A randomized, double-blinded placebo-controlled, 2 months, parallel-group clinical trial was conducted. Forty-three female patients (20-50 years) with mild to moderate RA were recruited and assigned into NS (n = 23) and placebo (n = 20) groups to receive one gram of NS oil, or starch, capsule in two divided doses, respectively. The disease activity scores of 28 joints (DAS28) were calculated and percentages of CD4(+), CD8(+), and CD4(+)CD25(+) T cells were examined using flow cytometry.

RESULTS: Treatment with NS led to significant reduction of the serum high-sensitivity C-reactive protein (hs-CRP) level and DAS-28 score and an improved number of swollen joints compared with baseline and placebo groups. A relatively comparable CD4(+) T cell percentage was observed in the NS and placebo groups either in baseline or the end of study. The treatment also resulted in reduced CD8(+), and increased CD4(+)CD25(+) T cell percentage and the CD4(+)/CD8(+) ratio as compared to placebo and baseline. A negative significant correlation between changes in CD8(+) and changes in CD4(+)CD25(+) T cells and a positive significant correlation between changes in CD4(+)CD25(+) T cells and changes in the CD4(+)/CD8(+) ratio was observed in the NS group.

CONCLUSION: This study gives strength to the potential relevance of NS in clinical management of RA through modulation of T lymphocytes.

Article Published Date : Apr 30, 2016

Study Type : Human Study

[39] Topical application of Nigella sativa oil was effective in reducing pain in patients with knee osteoarthritis

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Abstract Title:

Effect of Topical Application of Nigella Sativa Oil and Oral Acetaminophen on Pain in Elderly with Knee Osteoarthritis: A Crossover Clinical Trial.

Abstract Source:

Electron Physician. 2016 Nov ;8(11):3193-3197. Epub 2016 Nov 25. PMID: 28344755

Abstract Author(s):

Akram Kooshki, Reza Forouzan, Mohammad Hassan Rakhshani, Maryam Mohammadi

Article Affiliation:

Akram Kooshki

Abstract:

BACKGROUND: Limited evidence supports Nigella sativa's role as an effective complementary and alternative medicine and the anti-inflammatory effects of Nigella sativa on patients with allergic rhinitis.

OBJECTIVE: The aim of this study was to investigate the effect of topical application of Nigella sativa oil and oral acetaminophen on pain in the elderly with knee osteoarthritis residing in a parents' home in Sabzevar.

METHODS: This study is done as a crossover clinical trial. After obtaining written consent of elderly patients with osteoarthritis of the knee, they were randomly divided into two groups. In step 1, in group 1, 1 cc of Nigella sativa oil was applied on the knee joint every 8 hours for 3 weeks; for the second group, every 8 hours for 3 weeks, patients were given 1 tablet of 325 mg acetaminophen. After a period of 1 month without medication to wash out each group, in step 2, each treatment group received the drug interaction in the same way as above. Pain was determined using a visual scale (VAS) before and after the first and second stages. Treatment response was defined as a decrease in pain scores over 1.5. Data analysis was performed with an R software mixed model.

RESULTS: This study was done on 40 elderly patients: 18 (45%) men and 22 (55%) women. Their mean year and weight were 75.66±8.9 years and 69.67±14.33 kg, respectively. Study results showed that topical application of Nigella sativa oil and oral acetaminophen reduced pain in elderly with knee osteoarthritis; after using Nigella sativa oil, the reduction of pain was higher (p=0.01).

CONCLUSION: The results of this study showed that topical application of Nigella sativa oil was effective in reducing pain in patients with knee osteoarthritis; therefore, it is recommended as a safe supplement for these elderly.

TRIAL REGISTRATION: The trial was registered at TCTR (http://www.clinicaltrials.in.th/) with the ID: TCTR20160125003.

FUNDING: This study was approved and supported by the Sabzevar University of Medical Sciences.

Article Published Date : Oct 31, 2016

Study Type : Human Study

[40] A Nigella sativa extract showed a 88.3% inhibition of proliferation of SiHa human cervical cancer cells and increased the expression of caspase-3, -8 and -9 several-fold

Abstract Title:

Methanolic extract of Nigella sativa seed inhibits SiHa human cervical cancer cell proliferation through apoptosis.

Abstract Source:

Nat Prod Commun. 2013 Feb ;8(2):213-6. PMID: 23513732

Abstract Author(s):

Tarique N Hasan, Gowhar Shafi, Naveed A Syed, Muhammad A Alfawaz, Mohammed A Alsaif, Anjana Munshi, Kai Y Lei, Ali A Alshatwi

Article Affiliation:

Molecular Cancer Biology Research Lab, Dept. of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Saudi Arabia.

Abstract:

Nigella sativa (NS), also known as black cumin, has long been used in traditional medicine for treating various cancer conditions. In this study, we sought to investigate the potential anti-cancer effects of NS extract using SiHa human cervical cancer cells. NS showed an 88.3% inhibition of proliferation of SiHa human cervical cancer cells at a concentration of 125 microL/mL methanolic extract at 24 h, and an IC50 value 93.2 microL/mL. NS exposure increased the expression of caspase-3, -8 and -9 several-fold. The analysis of apoptosis by Dead End terminal transferase-mediated dUTP-digoxigenin end labeling (TUNEL) assay was used to further confirm that NS induced apoptosis. Thus, NS was concluded to induce apoptosis in SiHa cell through both p53 and caspases activation. NS could potentially be an alternative source of medicine for cervical cancer therapy.

Article Published Date : Jan 31, 2013

Study Type : Human In Vitro

[41] N. sativa oil could be used against multi-drug resistant S. aureus in the treatment of diabetic wounds

Abstract Title:

Antimicrobial activity of Nigella sativa L. seed oil against multi-drug resistant Staphylococcus aureus isolated from diabetic wounds.

Abstract Source:

Pak J Pharm Sci. 2015 Nov ;28(6):1985-90. PMID: 26639493

Abstract Author(s):

Lorina Badger Emeka, Promise Madu Emeka, Tahir Mehmood Khan

Article Affiliation:

Lorina Badger Emeka

Abstract:

Microbial resistance to existing antibiotics has led to an increase in the use of medicinal plants that show beneficial effects for various infectious diseases. The study evaluates the susceptibility of multidrug resistant Staphylococcus aureus to Nigella sativa oil. Staphylococcus aureus was isolated from 34 diabetic patient's wounds attending the Renaissance hospital, Nsukka, Southeast Nigeria. The isolates were characterized and identified using standard microbiological techniques. Isolates were cultured and a comparative In vitro antibiotic susceptibility test was carried out using the disk diffusion method. Of the 34 samples collected, 19(56%) showed multidrug resistance to the commonly used antibiotics. Nigella sativa oil was then studied for antibacterial activity against these multidrug resistant isolates of Staphylococcus aureus in varying concentration by well diffusion method. The oil showed pronounced dose dependent antibacterial activity against the isolates. Out of 19 isolates, 8(42%) were sensitive to undiluted oil sample; 4(21%) of these showed sensitivity at 200 mg/ml, 400 mg/ml and 800 mg/ml respectively. Eleven (58%) of the isolates were completely resistant to all the oil concentrations. The present study, reports the isolation of multi-drug resistant S. aureus from diabetic wounds and that more than half of isolates were susceptible to different concentrations N. sativa oil.

Article Published Date : Oct 31, 2015

Study Type : Human In Vitro

[42] Nigella sativa has successfully produced inhibition of growth of MCF cell lines with an equivalent potency as that of the standard cisplatin

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Abstract Title:

A study of Nigella sativa induced growth inhibition of MCF and HepG2 cell lines: An anti-neoplastic study along with its mechanism of action.

Abstract Source:

Pharmacognosy Res. 2015 Apr-Jun;7(2):193-7. PMID: 25829794

Abstract Author(s):

Y Padmanabha Reddy, K B Chandrasekhar, Mohammed Jaffar Sadiq

Article Affiliation:

Y Padmanabha Reddy

Abstract:

OBJECTIVE: To evaluate the anticancer potential of seeds of Nigella sativa using MCF and HepG2 cell lines along with its mechanism of action.

MATERIALS AND METHODS: (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and acridine orange/ethidium bromide nuclear staining technique were selected to evaluate anticancer potential and mechanism of action of test extract.

RESULTS: Aqueous extract of N.sativa at a test dose of 180 mg and 300 mg was identified to be the best as anticancer agent against MCF and HepG2 cell lines among different solvent test extract where doxorubicin and cisplatin were employed as standard references.

DISCUSSION: Further study including separation and characterization of active principles in the aqueous extract shall prove beneficial.

Article Published Date : Mar 31, 2015

Study Type : Human In Vitro

[43] Thymoquinone-rich fractions of Nigella sativa could be an alternative to combat oxidative stress insults in neurodegenerative diseases

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Abstract Title:

Modulation of Hydrogen Peroxide-Induced Oxidative Stress in Human Neuronal Cells by Thymoquinone-Rich Fraction and Thymoquinone via Transcriptomic Regulation of Antioxidant and Apoptotic Signaling Genes.

Abstract Source:

Oxid Med Cell Longev. 2016 ;2016:2528935. Epub 2015 Dec 28. PMID: 26823946

Abstract Author(s):

Norsharina Ismail, Maznah Ismail, Nur Hanisah Azmi, Muhammad Firdaus Abu Bakar, Hamidon Basri, Maizaton Atmadini Abdullah

Article Affiliation:

Norsharina Ismail

Abstract:

Nigella sativa Linn. (N. sativa) and its bioactive constituent Thymoquinone (TQ) have demonstrated numerous pharmacological attributes. In the present study, the neuroprotective properties of Thymoquinone-rich fraction (TQRF) and TQ against hydrogen peroxide- (H2O2-) induced neurotoxicity in differentiated human SH-SY5Y cells were investigated. TQRF was extracted using supercritical fluid extraction while TQ was acquired commercially, and their effects on H2O2 were evaluated using cell viability assay, reactive oxygen species (ROS) assay, morphological observation, and multiplex gene expression. Both TQRF and TQ protected the cells against H2O2 by preserving the mitochondrial metabolic enzymes, reducing intracellular ROS levels, preserving morphological architecture, and modulating the expression of genes related to antioxidants (SOD1, SOD2, and catalase) and signaling genes (p53, AKT1, ERK1/2, p38 MAPK, JNK, and NF-κβ). In conclusion, the enhanced efficacy of TQRF over TQ was likely due to the synergism of multiple constituents in TQRF. The efficacy of TQRF was better than that of TQ alone when equal concentrations of TQ in TQRF were compared. In addition, TQRF also showed comparable effects to TQ when the same concentrations were tested. These findings provide further support for the use of TQRF as an alternative to combat oxidative stress insults in neurodegenerative diseases.

Article Published Date : Dec 31, 2015

Study Type : Human In Vitro

[44] Nigella Sativa Concoction Induced Sustained Seroreversion in HIV Patient

Abstract Title:

Nigella Sativa Concoction Induced Sustained Seroreversion in HIV Patient.

Abstract Source:

PMID: 24311845

Abstract Author(s):

Abdulfatah Adekunle Onifade, Andrew Paul Jewell, Waheed Adeola Adedeji

Article Affiliation:

Immunology unit, College of Medicine, University of Ibadan, Nigeria.

Abstract:

Nigella sativa had been documented to possess many therapeutic functions in medicine but the least expected is sero-reversion in HIV infection which is very rare despite extensive therapy with highly active anti-retroviral therapy (HAART). This case presentation is to highlight the complete recovery and sero-reversion of adult HIV patient after treatment with Nigella sativa concoction for the period of six months. The patient presented to the herbal therapist with history of chronic fever, diarrhoea, weight loss and multiple papular pruritic lesions of 3 months duration. Examination revealed moderate weight loss, and the laboratory tests of ELISA (Genscreen) and western blot (new blot 1&2) confirmed sero-positivity to HIV infection with pre-treatment viral (HIV-RNA) load and CD4 count of 27,000 copies/ml and CD4 count of 250 cells/ mm(3) respectively. The patient was commenced on Nigella sativa concoction 10mls twice daily for 6 months.. He was contacted daily to monitor side-effects and drug efficacy. Fever, diarrhoea and multiple pruritic lesions disappeared on 5th, 7th and 20th day respectively on Nigella sativa therapy. The CD4 count decreased to 160 cells/ mm3 despite significant reduction in viral load (≤1000 copies/ml) on 30th day on N. sativa. Repeated EIA and Western blot tests on 187th day on Nigella sativa therapy was sero-negative. The post therapy CD4 count was 650cells/ mm(3) with undetectable viral (HIV-RNA) load. Several repeats of the HIV tests remained sero-negative, aviraemia and normal CD4 count since 24 months without herbal therapy. This case report reflects the fact that there are possible therapeutic agents in Nigella sativa that may effectively control HIV infection.

Article Published Date : Dec 31, 2012

Study Type : Human: Case Report

[45] "Nigella sativa (black cumin) seed extract alleviates symptoms of allergic diarrhea in mice, involving opioid receptors"

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Abstract Title:

Nigella sativa (black cumin) seed extract alleviates symptoms of allergic diarrhea in mice, involving opioid receptors.

Abstract Source:

PLoS One. 2012 ;7(6):e39841. Epub 2012 Jun 29. PMID: 22768141

Abstract Author(s):

Swantje C Duncker, David Philippe, Christine Martin-Paschoud, Mireille Moser, Annick Mercenier, Sophie Nutten

Article Affiliation:

Nestlé Research Center, Nestec Ltd., Vers-chez-les-Blancs, Lausanne, Switzerland. swantje.duncker@rdls.nestle.com

Abstract:

The incidence of food hypersensitivity and food allergies is on the rise and new treatment approaches are needed. We investigated whether N. sativa, one of its components, thymoquinone, or synthetic opioid receptor (OR)-agonists can alleviate food allergy. Hence, ovalbumin (OVA)-sensitized BALB/c-mice were pre-treated either with a hexanic N. sativa seed extract, thymoquinone, kappa-(U50'4889) or mu-OR-agonists (DAMGO) and subsequently challenged intra-gastrically with OVA. All 4 treatments significantly decreased clinical scores of OVA-induced diarrhea. N. sativa seed extract, thymoquinone, and U50'488 also decreased intestinal mast cell numbers and plasma mouse mast cell protease-1 (MMCP-1). DAMGO, in contrast, had no effect on mast cell parameters but decreased IFNγ, IL-4, IL-5, and IL-10 concentration after ex vivo re-stimulation of mesenteric lymphocytes. The effects on allergy symptoms were reversible by OR-antagonist pre-treatment, whereas most of the effects on immunological parameter were not. We demonstrate that N. sativa seed extract significantly improves symptoms and immune parameters in murine OVA-induced allergic diarrhea; this effect is at least partially mediated by thymoquinone. ORs may also be involved and could be a new target for intestinal allergy symptom alleviation. N. sativa seed extract seems to be a promising candidate for nutritional interventions in humans with food allergy.

Article Published Date : Dec 31, 2011

Study Type : Animal Study

[46] A combination of black seeds and turmeric at low doses showed enhanced efficacy in preventing metabolic syndrome in fructose fed rats

Abstract Title:

Coadministration of black seeds and turmeric shows enhanced efficacy in preventing metabolic syndrome in fructose-fed rats.

Abstract Source:

J Cardiovasc Pharmacol. 2015 Feb ;65(2):176-83. PMID: 25384193

Abstract Author(s):

Faridah Amin, Anwarul-Hassan Gilani, Malik Hassan Mehmood, Bina S Siddiqui, Nasima Khatoon

Article Affiliation:

Faridah Amin

Abstract:

Among noncommunicable diseases, metabolic syndrome (MS), a cluster of metabolic disorders including obesity, hyperglycemia, hyperlipidemia and hypertension, is highly prevalent in modern society. Its management requires lifestyle modifications and/or the life-long use of multiple medications, hence demanding development of safe alternative remedies. This study was aimed to establish the efficacy of combined use of black seeds and turmeric using fructose-fed rat model of MS. The high-performance liquid chromatographic fingerprints of turmeric and black seeds showed the presence of curcumin and thymoquinone, respectively, as their major constitutes. Different doses of black seeds and turmeric, individually and in combination, were administered to fructose-fed rats for up to 6 weeks representing characteristic features of MS. At 3 weeks of the treatment, black seeds and turmeric lowered (P<0.01) high blood pressure and low-density lipoprotein cholesterol, respectively, whereas their coadministration reduced (P<0.01) both high blood pressure and hypertriglyceridemia. At 6 weeks, the coadministration of both herbs, at half the doses of individual herbs, was the most effective (P<0.001) in preventing hypertension, hyperglycemia, dyslipidemia, hyperinsulinemia, and endothelial dysfunction than the individual herbs. This study demonstrates the therapeutic superiority of the combination of black seeds and turmeric at low doses over individually tested herbs, in improving features of MS.

Article Published Date : Jan 31, 2015

Study Type : Animal Study

[47] A hydro-alcoholic extract of nigella sativa reduced the LPS-induced sickness behaviors in rats

Abstract Title:

The effects of Nigella sativa on sickness behavior induced by lipopolysaccharide in male Wistar rats.

Abstract Source:

Avicenna J Phytomed. 2016 Jan-Feb;6(1):104-16. PMID: 27247927

Abstract Author(s):

Fatemeh Norouzi, Azam Abareshi, Akbar Anaeigoudari, Mohammad Naser Shafei, Zahra Gholamnezhad, Mohsen Saeedjalali, Reza Mohebbati, Mahmoud Hosseini

Article Affiliation:

Fatemeh Norouzi

Abstract:

OBJECTIVE: Neuroimmune factors contribute on the pathogenesis of sickness behaviors. Nigella sativa (NS) has anti-inflammatory, anti-anxiety and anti-depressive effects. In the present study, the effect of NS hydro-alcoholic extract on sickness behavior induced by lipopolysaccharide (LPS) was investigated.

MATERIALS AND METHODS: The rats were divided into five groups (n=10 in each): (1) control (saline), (2) LPS (1 mg/kg, administered two hours before behavioral tests), (3-5) LPS-Nigella sativa 100 , 200 and 400 mg/kg (LPS-NS 100, LPS-NS 200 and LPS-NS 400, respectively). Open- field (OF), elevated plus maze (EPM) and forced swimming test (FST) were performed.

RESULTS: In OF, LPS reduced the peripheral crossing, peripheral distance, total crossing and total distance compared to control (p<0.01- p<0.001). The central crossing, central distance and central time in LPS-NS 100, LPS-NS200 and LPS-NS 400 groups were higher than LPS (p<0.01- p<0.001). In EPM, LPS decreased the open arm entries, open arm time and closed arm entries while increased the closed time compared to control (p<0.001). Pretreatment by NS extract reversed the effects of LPS (p<0.05- p<0.001). In FST, LPS increased the immobility time while, decreased the climbing and active times compared to control (p<0.05- p<0.001). In LPS-NS 100, LPS-NS 200 and LPS-NS 400 groups the immobility time was less while, the active and climbing times were more than those of LPS (p<0.05- p<0.001).

CONCLUSION: The results of the present study showed that the hydro-alcoholic extract of NS reduced the LPS-induced sickness behaviors in rats. Further investigations are required for better understanding the responsible compound (s) and the underlying mechanism(s).

Article Published Date : Dec 31, 2015

Study Type : Animal Study

[48] A hydroalcoholic extract of the Nigella sativa decreased oxidative stress in hippocampus of the STZ-induced diabetic rats

Abstract Title:

The effects of hydroalcoholic extract of Nigella sativa seed on oxidative stress in hippocampus of STZ-induced diabetic rats.

Abstract Source:

Avicenna J Phytomed. 2015 Jul-Aug;5(4):333-40. PMID: 26445713

Abstract Author(s):

Abbasali Abbasnezhad, Parichehr Hayatdavoudi, Saeed Niazmand, Maryam Mahmoudabady

Article Affiliation:

Abbasali Abbasnezhad

Abstract:

OBJECTIVE: Oxidative stress plays an important role in the etiology of diabetic complications. Diabetes impairs hippocampus neurogenesis, synaptic plasticity, and learning. The aim of this study was to investigate the effects of hydroalcoholic extract of Nigella sativa seed on oxidative stress in STZ-induced diabetic rats' hippocampus.

MATERIALS AND METHODS: Diabetes induced by 60 mg/kg STZ, i.p, and the rats were divided into five experimental groups (n=8-10 in each group) including control (received 0.5 ml normal saline), untreated STZ-diabetic (received 0.5 ml normal saline), and treated rats received Nigella sativa extract (200 and 400 mg/kg) or metformin (300 mg/kg) by gavage for 42 days. Serum glucose concentration and body weight as well as hippocampus tissue malondialdehyde and thiol levels were determined by calorimetric assay.

RESULTS: Serum glucose level in the diabetic rats treated with 200 mg/kg Nigella sativa extract at the days 24 and 45 decreased in comparison to untreated diabetic group (p<0.05, p<0.01, respectively). Weight loss was significantly different between metformin and Nigella sativa extract at the dose of 200 and 400 mg/kg (p<0.05). Thiol content of hippocampus increased by 200 mg/kg Nigella sativa extract in comparison to untreated diabetic group (p<0.05). Malondialdehyde content of hippocampus reduced by Nigella sativa extract, 200 mg/kg (p<0.001), 400 mg/kg (p<0.05), and metformin (p<0.05) in comparison to the untreated diabetic group.

CONCLUSION: The results of the present study showed that hydroalcoholic extract of the Nigella sativa decreased oxidative stress in hippocampus of the STZ-induced diabetic rats. Nigella sativa at the dose of 200 mg/kg was more effective to reduce oxidative stress in hippocampus of rats.

Article Published Date : Jun 30, 2015

Study Type : Animal Study

[49] A liposomal preparation of black seed oil demonstrated significant analgesic activity in mice

Article Publish Status: FREE

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Abstract Title:

The impact of formulation attributes and process parameters on black seed oil loaded liposomes and their performance in animal models of analgesia.

Abstract Source:

Saudi Pharm J. 2017 Mar ;25(3):404-412. Epub 2016 Oct 1. PMID: 28344496

Abstract Author(s):

Zerin T Rushmi, Nasrin Akter, Rabeya J Mow, Merina Afroz, Mohsin Kazi, Marcel de Matas, Mahbubur Rahman, Mohammad H Shariare

Article Affiliation:

Zerin T Rushmi

Abstract:

This study aimed to formulate black seed oil (Nigella sativa) loaded liposomes using the ethanol injection method to enhance oral bioavailability and improve therapeutic activity in small animal studies of analgesia. The impact of formulation attributes and process parameters on the liposomal system was evaluated with key quality attributes being particle size, morphology, and entrapment efficiency. The particle size and entrapment efficiency of the liposome preparation were found to be between the range of 50-900 nm and 34-87% respectively. Particle size distribution data suggested that increasing the percentage of oil, up to a certain concentration, reduced the size of the liposomes significantly from 520 ± 81.2 nm to 51.48 ± 1.31 nm. Stirring and injection rate were shown to have marked impact onthe average particle size of liposome. It was observed that entrapment efficiency of liposomes was greatly influenced by the amount of cholesterol and type of cryoprotectant used during formulation. The stability study indicated that the liposomal preparation was stable at ambient conditions for one month. In vivo studies showed that the liposomal preparation demonstrated significant analgesic activity in mice.

Article Published Date : Feb 28, 2017

Study Type : Animal Study

[50] A plant mixture comprising Nigella sativa, Myrrh, Gum Olibanum (Frankincense), Gum Asafoetida and Aloe lowers blood sugar by inhibiting hepatis gluconeogenesis

Abstract Title:

The effect of a plants mixture extract on liver gluconeogenesis in streptozotocin induced diabetic rats.

Abstract Source:

Diabetes Res. 1991 Dec;18(4):163-8. PMID: 1842751

Abstract Author(s):

F al-Awadi, H Fatania, U Shamte

Abstract:

We have previously reported on plant mixture extract comprising of Nigella sativa, Myrrh, Gum Olibanum, Gum Asafoetida and Aloe to have a blood glucose lowering effect. The present study with streptozotocin diabetic rats is focussed on the mechanism of action, specifically on a) hepatic gluconeogenesis b) activity of key gluconeogenic enzymes, pyruvate carboxylase (PC) and phosphoenol-pyruvate carboxykinase (PEPCK). Similar studies using a biguanide, phenformin, have been conducted to compare the mode of action of these two compounds. The blood glucose levels (mean +/- SEM) before and after treatment with the plants extract were (16.7 +/- 1.7 mmol/L and 8.5 +/- 1.3 mmol/L) and with phenformin (15.1 +/- 1.3 mmol/L and 10.7 +/- 1.5 mmol/L). The rate of gluconeogenesis in isolated hepatocytes as well as activity of PC and PEPCK in liver homogenates is significantly lowered following treatment with the plants extract. Although phenformin also lowers blood glucose, it does not affect hepatic gluconeogenesis under stated experimental conditions. It is concluded that the anti-diabetic action of the plants extract may, at least partly, be mediated through decreased hepatic gluconeogenesis. The extract may prove to be a useful therapeutic agent in the treatment of non-insulin dependent diabetes mellitus (NIDDM).

Article Published Date : Dec 01, 1991

Study Type : Animal Study

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[51] A thymoquinone-rich fraction extracted from Nigella sativa effectively improved the plasma and liver antioxidant capacity

Abstract Title:

Nigella sativa thymoquinone-rich fraction greatly improves plasma antioxidant capacity and expression of antioxidant genes in hypercholesterolemic rats.

Abstract Source:

Free Radic Biol Med. 2010 Mar 1 ;48(5):664-72. Epub 2009 Dec 11. PMID: 20005291

Abstract Author(s):

Maznah Ismail, Ghanya Al-Naqeep, Kim Wei Chan

Article Affiliation:

Maznah Ismail

Abstract:

The antioxidant activities of the thymoquinone-rich fraction (TQRF) extracted from Nigella sativa and its bioactive compound, thymoquinone (TQ), in rats with induced hypercholesterolemia were investigated. Rats were fed a semipurified diet supplemented with 1% (w/w) cholesterol and were treated with TQRF and TQ at dosages ranging from 0.5 to 1.5 g/kg and 20 to 100 mg/kg body wt, respectively, for 8 weeks. The hydroxyl radical (OH(.))-scavenging activity of plasma samples collected from experimental rats was measured by electron spin resonance. The GenomeLab Genetic Analysis System was used to study the molecular mechanism that mediates the antioxidative properties of TQRF and TQ. Plasma total cholesterol and low-density-lipoprotein cholesterol levels were significantly decreased in the TQRF- and TQ-treated rats compared to untreated rats. Feeding rats a 1% cholesterol diet for 8 weeks resulted in a significant decrease in plasma antioxidant capacity, as measured by the capacity to scavenge hydroxyl radicals. However, rats treated with TQRF and TQ at various doses showed significant inhibitory activity toward the formation of OH(.) compared to untreated rats. Upon examination of liver RNA expression levels, treatment with TQRF and TQ caused the up-regulation of the superoxide dismutase 1 (SOD1), catalase, and glutathione peroxidase 2 (GPX) genes compared to untreated rats (P<0.05). In support of this, liver antioxidant enzyme levels, including SOD1 and GPX, were also apparently increased in the TQRF- and TQ-treated rats compared to untreated rats (P<0.05). In conclusion, TQRF and TQ effectively improved the plasma and liver antioxidant capacity and enhanced the expression of liver antioxidant genes of hypercholesterolemic rats.

Article Published Date : Feb 28, 2010

Study Type : Animal Study

[52] Active components in Nigella sativa and Olive oil may prove useful in treating impaired cognition

Abstract Title:

Neurochemical and behavioral effects of Nigella sativa and Olea europaea oil in rats.

Abstract Source:

Nutr Neurosci. 2016 Nov 21:1-10. Epub 2016 Nov 21. PMID: 27868798

Abstract Author(s):

M Atif Raza Cheema, Shazia Nawaz, Sumera Gul, Tabinda Salman, Sabira Naqvi, Ahsana Dar, Darakhshan J Haleem

Article Affiliation:

M Atif Raza Cheema

Abstract:

OBJECTIVES: In the last few decades, therapeutic uses of medicinal compounds present in food as a normal constituent has risen substantially, largely because of their fewer side effects and adequate efficacy. This study is designed to investigate a role of brain serotonin (5-HT) and dopamine (DA) in the potential nootropic, anxiolytic, and other beneficial effects of Nigella sativa (NS) and Olea europaea (OE) oil in rat models.

METHODS: Animals were treated with NS and OE oil orally at doses of 0.1 ml/kg and 0.25 ml/kg for 5 weeks. Food intake and body weight change, anxiety-like effects in elevated plus maze and activity in a novel and familiar environment were monitored weekly. Effects on learning and memory after 5 weeks treatment were monitored using Morris water maze test. Neurochemical analysis was carried using HPLC-ECD method.

RESULTS: NS and OE oil administration enhanced learning and memory in Morris water maze test and the effects were greater in NS than OE oil-treated animals. Low dose of OE oil increased exploration in an open field, higher dose of OE oil and both doses of NS oil produced no consistent effect on open field exploration. Effects of both oils on anxiety-like behavior, food and water intake, and activity in activity box were either not consistent or did not occur. The treatment increased homovanillic acid (HVA). 5-HT levels increased in high dose of NS oil and low dose of OE oil-treated groups. Low dose NS oil decreased 5-HT.

DISCUSSION: The present study suggests that active components in NS and OE oil may prove useful in treating impaired cognition. OE oil may produce psychostimulant-like effect. Modulation of DA and serotonin neurotransmission seems important in the pharmacological effect of these oils.

Article Published Date : Nov 20, 2016

Study Type : Animal Study

[53] Administration of either nigella sativa oil counteracts dexamethasone induced-gastric lesion

Abstract Title:

Gastroprotective Effects of Montelukast and Nigella Sativa Oil against Corticosteroids- Induced Gastric Damage: Much More Than Antiasthmatic Drugs.

Abstract Source:

Can J Physiol Pharmacol. 2017 Feb 10. Epub 2017 Feb 10. PMID: 28187265

Abstract Author(s):

Fatma Hassan Rizk, Marwa Awad Ibrahim, Marwa Abd-Elsalam, Nema Ali Soliman, Sherief Abd-Elsalam

Article Affiliation:

Fatma Hassan Rizk

Abstract:

Corticosteroids are used to treat variety of diseases like bronchial asthma. However, long term corticosteroids have a gastric ulcerogenic potential. Montelukast (MTK) and nigella sativa oil (NSO) are used in treatment of bronchial asthma. Previous studies showed that MTK and NSO had gastroprotective effects in other models of gastric ulcer. The present study assesses synergistic gastroprotective effects of both drugs in dexamethasone (DXM)-induced gastric damage. 50 male rats were divided into 5 groups; normal control (I), DXM group (II), MTK+DXM group (III), NSO+DXM group (IV), MTK+NSO+DXM group (V). After 7 days, stomachs were removed for biochemical analysis and histological examinations. Significant increases in MDA level, SOD activity, MPO activity, and proliferating cell nuclear antigen (PCNA) positive cells, with significant decreases in mucus secretion were detected in DXM-treated group compared with group I. While, significant decreases of MDA level, MPO activity, and PCNA positive cells and significant increases in mucus secretion were detected in treated groups compared with group I and II. SOD activity significantly decreased in group V compared with group II only. We could conclude that administration of either MTK or NSO or both with DXM counteracts DXM induced-gastric lesions.

Article Published Date : Feb 09, 2017

Study Type : Animal Study

[54] Administration of melatonin, retinoic acid and Nigella sativa reduced the carcinogenic effects of DMBA

Article Publish Status: FREE

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Abstract Title:

The biochemical and morphological alterations following administration of melatonin, retinoic acid and Nigella sativa in mammary carcinoma: an animal model.

Abstract Source:

Int J Exp Pathol. 2005 Dec ;86(6):383-96. PMID: 16309544

Abstract Author(s):

Mohamad A Abd el-Aziz, Hosny A Hassan, Mahmoud H Mohamed, Abdel-Raheim M A Meki, Sary K H Abdel-Ghaffar, Mahmoud R Hussein

Article Affiliation:

Mohamad A Abd el-Aziz

Abstract:

Worldwide, breast cancer is the second leading cause of cancer death among women and the third most common cancer. Although our understanding of the molecular basis of this fatal disease has improved, this malignancy remains elusive. Melatonin (Mel), retinoic acid (RA) and Nigella sativa (NS) are substances with anticancer effects. To date, our understanding of the mechanisms of therapeutic effects of these products in mammary cancer is still marginal. To look at the preventive and therapeutic values of these products, we carried out this investigation. An animal model formed of 80 rats was established. The animals were divided into eight groups of 10 animals each: (a) control group injected with the same vehicle used for treatments in the relevant dosages and routes; (b) carcinogen group injected with the known carcinogenic substance 7,12-di-methylbenz(a)anthracene (DMBA) that induces mammary carcinoma; (c) three prophylactic (Pro) groups (Mel-Pro, RA-Pro and NS-Pro) injected with test substances (Mel, RA and NS, respectively) 14 days before the intake of the carcinogenic substance DMBA and then continued until the end of the experiments; and (d) three treated (Tr) groups (Mel-Tr, RA-Tr and NS-Tr) injected with the vehicles after the intake of DMBA. In both the Pro and Tr groups, the drugs were daily administered for 3 months. The animals were killed, and their serum and tissues were evaluated for (a) markers of tumorigenicity [serum levels of total sialic acid (TSA) and lipid-bound sialic acid (LSA)], (b) markers of endocrine derangement (serum prolactin, estradiol and progesterone levels), (c) apoptotic changes [serum tumour necrosis factor (TNF)-alpha, tissue caspase-3 activity, percentage of DNA fragmentation and ultrastructural features of apoptosis] and (d) markers of oxidative stress (tissue levels of lipid peroxides and nitric oxide). Carcinoma was absent both in the control and in the NS-Pro groups. Mammary carcinoma occurred in DMBA and other Pro and Tr groups. The frequency of mammary carcinoma was high in the carcinogen DMBA group (60%), followed by the Tr (56%) and finally the Pro groups (33%). These tumours included papillary, comedo and cribriform carcinomas. As compared with the control group, the development of carcinoma in the carcinogen DMBA group was associated with increased levels of (a) markers of tumorigenicity (77.0 +/- 3.3 vs. 209.0 +/- 5.6 and P<0.05 for TSA; 28.7 +/- 1.7 vs. 41.8 +/- 1.2 and P<0.01 for LSA), (b) markers of endocrine derangement (2.5 +/- 0.1 vs. 3.6 +/- 0.3 and P<0.05 for prolactin; 39.6 +/- 1.3 vs. 24.8 +/- 2.1 and P<0.01 for progesterone and 31.0 +/- 0.7 vs. 51.1 +/- 3.4 and P<0.01 for estradiol) and (c) markers of oxidative stress (2.3 +/- 0.2 vs. 5.2 +/- 0.7 and P<0.01 for lipid peroxides and 4.4 +/- 0.2 vs. 7.6 +/- 0.8 and P<0.01 for nitric oxide). Also, it was associated with decreased levels of markers of apoptotic activity (20.8 +/- 1.1 vs. 13.4 +/- 0.7 and P<0.01 for caspase-3; 29.0 +/- 1.7 vs. 20.9 +/- 1.3 and P<0.05 for percentage of DNA fragmentation; and 9.4 +/- 0.8 vs. 52.1 +/- 3.3 and P<0.01 for TNF-alpha). When compared with the carcinogen DMBA group, the development of carcinoma in the Pro and Tr groups was associated with decreased levels of (a) markers of tumorigenicity, (b) markers of endocrine derangement and (c) markers of oxidative stress. Alternatively, carcinogenicity was associated with statistically significant (P<0.01) increased levels of markers of apoptotic activity. To conclude, the administration of Mel, RA and NS reduced the carcinogenic effects of DMBA, suggesting a protective role. The possible underlying mechanisms of these effects await further investigations.

Article Published Date : Nov 30, 2005

Study Type : Animal Study

[55] Administration of Nigella sativa can be used to prevent formaldehyde induced apoptosis and epithelial damage

Abstract Title:

Harmful effects of formaldehyde and possible protective effect of Nigella sativa on the trachea of rats.

Abstract Source:

Niger J Clin Pract. 2017 May ;20(5):523-529. PMID: 28513508

Abstract Author(s):

E Sapmaz, H I Sapmaz, N Vardi, U Tas, M Sarsilmaz, Y Toplu, A Arici, M Uysal

Article Affiliation:

E Sapmaz

Abstract:

OBJECTIVE: We aimed in this study to investigate the harmful effects of formaldehyde (FA) inhalation and possible protective effects of Nigella sativa (NS) on rats' trachea.

MATERIALS AND METHODS: In this study, 63 adult male rats were used. Animals were divided into nine groups. Group I was used as control group. All other groups were exposed to FA inhalation. Group III, V, VII, and IX were administered NS by gavage. Tissues were examined histologically, and immunohistochemical examination for Bax and caspase-3 immunoreactivity was carried out.

RESULTS: Our study demonstrated that FA caused apoptosis in the tracheal epithelial cells. The most apoptotic activity occurred at a 10 ppm dose in a 13-week exposure. Distortion of tracheal epithelium and cilia loss on epithelial surface was present in all groups. However, NS treated Groups VII and IX had decreased apoptotic activity and lymphoid infiltration and protected the epithelial structure, despite some shedded areas. Difference of tracheal epithelial thickness and histological score was statistically significant between Group VI-VII and VIII-IX.

CONCLUSION: FA induces apoptosis and tracheal epithelial damage in rats, and chronic administration of NS can be used to prevent FA-induced apoptosis and epithelial damage.

Article Published Date : Apr 30, 2017

Study Type : Animal Study

[56] Administration of Nigella sativa seed oil exerts a protective effect against urolithiasis

Abstract Title:

NEPHROPROTECTIVE AND DIURETIC EFFECT OF NIGELLA SATIVA L SEEDS OIL ON LITHIASIC WISTAR RATS.

Abstract Source:

Afr J Tradit Complement Altern Med. 2016 ;13(6):204-214. Epub 2016 Sep 29. PMID: 28480381

Abstract Author(s):

Abdelkader Benhelima, Zohra Kaid-Omar, Houari Hemida, Tarek Benmahdi, Ahmed Addou

Article Affiliation:

Abdelkader Benhelima

Abstract:

BACKGROUND AND OBJECTIVE: The purpose of the current investigation was to study the influences, preventive and diuretic, of Nigella sativa L. seeds oil (NSSO) on calcium oxalate (CaOx) urolithiasis induced in Wistar male rats.

METHODOLOGY: Seeds of Nigella sativa L. (N.S) were analysed for the evaluation of the concentration of oxalate and calcium. Nigella sativa L. seeds oil is obtained by hydrodistillation and HPTLC densitometric method was adopted to determine the amount of thymoquinone (TQ) present. Thirty male Wistar rats were divided into 5 groups (N=6). Group I, negative control, drank tap water. The other groups were II Positive control, III, IV and V received a treatment model inducing calcium oxalate urolithiasis for 28 days, using an aqueous solution involve 0.75% (EG) ethylene glycol and 1.0 % (AC) chloride ammonium. Rats in group III received in addition, 750 mg/kg Cystone from the beginning to the end of calculi induction experimentation. However, rats in Groups IV and V received (NSSO) at 5 ml/kg b.w by gavage on days 1(st) to 28(th) and 15(th) to 28(th) days, respectively. On days 0, 7, 14, 21 and 28, body weights were measured and the 24-hour urine samples were accumulated and analysed for biochemical elements. On the 28(th) day, blood samples were collected for the estimation of serum parameters including creatinine, BUN and uric acid. All animals were sacrificed at the end of the experiment and the kidneys were detached for histopathological examination.

RESULTS: Administration of (NSSO) at 5 ml/kg body weight/dose/day for 28 days exerts a protective effect by reducing significantly (p<0.01) urinary and serum rates of calcium, phosphate and oxalate. This preventive diet could increase the volume of urine excreted.

CONCLUSION: The nephroprotectrice and diuretic activity demonstrated by Nigella sativa L. gives a scientific basis that approves their traditional use like a remedy against urolithiasis. List of Abbreviations:NSSO: Nigella sativa L. Seeds oil ; CaOx: Calcium Oxalate; N.S: Nigella sativa L.; HPTLC: High performance thin layer chromatography; TQ: Thymoquinone; N: Number; EG: Ethylene Glycol; AC: Chloride Ammonium BUN: Blood Urea Nitrogen; LD50: Lethal Dose 50; b.w: body weight; H&E: Haematoxyline and Eosin; HPLC-UV:;Caph: calcium phosphate; FR: glomerular filtration rate.

Article Published Date : Dec 31, 2015

Study Type : Animal Study

[57] Black cumin exhibit significant antifungal activity against candidasis in mice

Abstract Title:

The in vivo antifungal activity of the aqueous extract from Nigella sativa seeds.

Abstract Source:

Phytother Res. 2003 Feb;17(2):183-6. PMID: 12601685

Abstract Author(s):

M A U Khan, M K Ashfaq, H S Zuberi, M S Mahmood, A H Gilani

Article Affiliation:

Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi-74800, Pakistan.

Abstract:

The effect of an aqueous extract of Nigella sativa seeds was studied on candidiasis in mice. An intravenous inoculum of Candida albicans produced colonies of the organism in the liver, spleen and kidneys. Treatment of mice with the plant extract (6.6 mL/kg equivalent to 5 mg of estimated protein, once daily for 3 days) 24 h after the inoculation caused a considerable inhibitory effect on the growth of the organism in all organs studied. A 5-fold decrease in Candida in kidneys, 8-fold in liver and 11-fold in spleen was observed in the groups of animals post-treated with the plant extract. Histopathological examination of the respective organs confirmed these findings. These results indicate that the aqueous extract of Nigella sativa seeds exhibits inhibitory effect against candidiasis and this study validates the traditional use of the plant in fungal infections.

Article Published Date : Feb 01, 2003

Study Type : Animal Study

[58] Black cumin extract reduces the damage caused by cadmium poisoning in rats

Abstract Title:

Effect of black cumin (Nigella sativa) on heart rate, some hematological values, and pancreatic beta-cell damage in cadmium-treated rats.

Abstract Source:

Biol Trace Elem Res. 2006 May;110(2):151-62. PMID: 16757843

Abstract Author(s):

Halit Demir, Mehmet Kanter, Omer Coskun, Yesim Hulya Uz, Ahmet Koc, Abdulmelik Yildiz

Abstract:

This study was designed to investigate the effect of Nigella sativa (NS) on the heart rate, some hematological values, and pancreatic beta-cell damage in cadmium (Cd)-treated rats. The rats were randomly grouped into one of three experimental groups: Control, Cd treated, and Cd + NS treated. Each group contained 10 animals. The Cd-treated and Cd + NS-treated groups were injected subcutaneously daily with CdCl2 dissolved in isotonic NaCl in the amount of 2 mL/kg for 30 d, resulting in a dosage of 0.49 mg Cd/kg/d. The control group was injected with only isotonic NaCl (2 mL/kg/d) throughout the experiment (for 30 d). Three days prior to administration of CdCl2, the Cd + NS-treated group received the daily intraperitoneal (ip) injection of 2 mL/kg NS until the end of the study; animals in all three groups were fasted for 12 h and blood samples were taken for the determination of the glucose and insulin levels, red blood cell (RBC) and white blood cell (WBC) counts, packet cell volume (PCV), and hemoglobin (Hb) concentration. The heart rates of rats were also measured by a direct writing electrocardiograph before the blood withdrawals. It was found that NS treatment increased the lowered insulin levels, RBC and WBC counts, PCV, and neutrophil percentage in Cd-treated rats. However, the WBC count of Cd-treated rats with NS treatment was still lower than those of control values. NS treatment also decreased the elevated heart rate and glucose concentration of Cd-treated rats. Pancreatic tissues were also harvested from the sacrificed animals for morphological and immunohistochemical examinations. Cd exposure alone caused a degeneration, necrosis, and weak degranulation in the beta-cells of the pancreatic islets. In Cd + NS-treated rats, increased staining of insulin and preservation of islet cells were apparent. It is concluded that NS treatment might decrease the Cd-treated disturbances on heart rate, some hematological values, and pancreatic beta-cell.

Article Published Date : May 01, 2006

Study Type : Animal Study

[59] Black Cumin improves blood lipid profiles

Abstract Title:

Effect of Nigella sativa (kalonji) on serum cholesterol of albino rats.

Abstract Source:

Neurosci Lett. 2010 Oct 4;482(3):183-7. Epub 2010 Jun 11. PMID: 16092657

Abstract Author(s):

Amir Hamzo Dahri, Atta Muhammad Chandiol, Ali Akbar Rahoo, Rafique Ahmed Memon

Abstract:

BACKGROUND: The increased level of LDL-c in the serum has a high risk and the increased serum HDL-c level has a low risk for the development of atherosclerosis. The effect of Nigella sativa on levels of cholesterol fractions were determined in this study on rats. METHODS: 24 albino rats of 08 weeks age having equal number of males and females were kept at optimum atmospheric condition. The blood samples were taken at the start and different control and experimental diets were given for 20 weeks. The experimental diets were added with Nigella sativa as 30 mg/kg body weight. The blood samples were taken at the end of study. The blood samples drawn at the start and end of the study were estimated for serum cholesterol. The results of control and experimental groups were compared. RESULTS: Total serum cholesterol in the control group showed increase from 8.3+/-3.30 to 13.96+/-9.3 at 20 weeks. The serum HDL cholesterol showed increase from 44.4+/-6.12 to 80.45+/-5.95 level at 20 weeks. The serum LDL cholesterol showed increase from 8.3+/-3.30 to 13.96+/-9.3 at 20 weeks. The total serum cholesterol in experimental group was increased from 76.9+/-6.5 to 117.5+/-6.65 at 20 weeks. The serum HDL cholesterol levels was increased from 41.7+/-4.9 to 83.42+/-5.92 at 20 weeks as compared with control group. The LDL cholesterol levels were decreased from 12.7+/-6.9 to 8.5+/-7.8 at 20 weeks. CONCLUSION: This study shows significant decrease in serum low density lipoprotein cholesterol level, and increase in serum high density lipoprotein cholesterol levels.

Article Published Date : Oct 04, 2010

Study Type : Animal Study

[60] Black cumin may have antidiabetic effects by increasing insulin sensitivity

Abstract Title:

The In Vivo Antidiabetic Activity of Nigella sativa Is Mediated through Activation of the AMPK Pathway and Increased Muscle Glut4 Content.

Abstract Source:

Evid Based Complement Alternat Med. 2011;2011:538671. Epub 2011 Apr 14. PMID: 21584245

Abstract Author(s):

Ali Benhaddou-Andaloussi, Louis Martineau, Tri Vuong, Bouchra Meddah, Padma Madiraju, Abdellatif Settaf, Pierre S Haddad

Article Affiliation:

Department of Pharmacology and Montreal Diabetes Research Center, Université de Montréal, Montréal, QC, Canada H3C 3J7.

Abstract:

The antidiabetic effect of N. sativa seed ethanol extract (NSE) was assessed in Meriones shawi after development of diabetes. Meriones shawi were divided randomly into four groups: normal control, diabetic control, diabetic treated with NSE (2 g eq plant/kg) or with metformin (300 mg/kg) positive control, both administered by daily intragastric gavage for 4 weeks. Glycaemia and body weight were evaluated weekly. At study's end, an Oral Glucose Tolerance Test (OGTT) was performed to estimate insulin sensitivity. Upon sacrifice, plasma lipid profile, insulin, leptin, and adiponectin levels were assessed. ACC phosphorylation and Glut4 protein content were determined in liver and skeletal muscle. NSE animals showed a progressive normalization of glycaemia, albeit slower than that of metformin controls. Moreover, NSE increased insulinemia and HDL-cholesterol, compared to diabetic controls. Leptin and adiponectin were unchanged. NSE treatment decreased OGTT and tended to decrease liver and muscle triglyceride content. NSE stimulated muscle and liver ACC phosphorylation and increased muscle Glut4. These results confirm NSE'spreviously reported hypoglycaemic and hypolipidemic activity. More significantly, our data demonstrate that in vivo treatment with NSE exerts an insulin-sensitizing action by enhancing ACC phosphorylation, a major component of the insulin-independent AMPK signaling pathway, and by enhancing muscleGlut4 expression.

Article Published Date : Jan 01, 2011

Study Type : Animal Study

[61] Black cumin oil decreased the proinflammatory cytokines, lactate dehydrogenase, triglyceride, and cholesterol, which were increased in colitis

Abstract Title:

Protective effects of black cumin (Nigella sativa) oil on TNBS-induced experimental colitis in rats.

Abstract Source:

Dig Dis Sci. 2011 Mar ;56(3):721-30. Epub 2010 Jul 24. PMID: 20658190

Abstract Author(s):

F Isik, Tugba Tunali Akbay, A Yarat, Z Genc, R Pisiriciler, E Caliskan-Ak, S Cetinel, A Altıntas, G Sener

Article Affiliation:

F Isik

Abstract:

BACKGROUND: The pathogenesis and treatment of ulcerative colitis remain poorly understood. The aim of the present study is to investigate the effects of black cumin (Nigella sativa) oil on rats with colitis.

METHODS: Experimental colitis was induced with 1 mL trinitrobenzene sulfonic acid (TNBS) in 40% ethanol by intracolonic administration with 8-cm-long cannula under ether anesthesia to rats in colitis group and colitis + black cumin oil group. Rats in the control group were given saline at the same volume by intracolonic administration. Black cumin oil (BCO, Origo"100% natural Black Cumin Seed Oil,"Turkey) was given to colitis + black cumin oil group by oral administration during 3 days, 5 min after colitis induction. Saline was given to control and colitis groups at the same volume by oral administration. At the end of the experiment, macroscopic lesions were scored and the degree of oxidant damage was evaluated by colonic total protein, sialic acid, malondialdehyde, and glutathione levels, collagen content, and tissue factor, superoxide dismutase, and myeloperoxidase activities. Tissues were also examined by histological and cytological analysis. Proinflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, and IL-6], lactate dehydrogenase activity, and triglyceride and cholesterol levels were analyzed in blood samples.

RESULTS: We found that black cumin oil decreased the proinflammatory cytokines, lactate dehydrogenase, triglyceride, and cholesterol, which were increased in colitis.

CONCLUSIONS: BCO, by preventing inflammatory status in the blood, partly protected colonic tissue against experimental ulcerative colitis.

Article Published Date : Feb 28, 2011

Study Type : Animal Study

[62] Black cumin protects against chemical weapon agent induced lung injury

Abstract Title:

The protective effect of Nigella sativa on lung injury of sulfur mustard-exposed Guinea pigs.

Abstract Source:

Exp Lung Res. 2008 May;34(4):183-94. PMID: 18432455

Abstract Author(s):

Boskabady Mohammad Hossein, Vahedi Nasim, Amery Sediqa

Article Affiliation:

Department of Physiology and Pharmacological Research Center of Medicinal Plants, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran. boskabadymh@mums.ac.ir

Abstract:

The lung is one of the most exposable organs to chemical warfare agents such as sulfur mustard (SM) gas. Airway hyperresponsiveness and lung inflammation are reported in chemical warfare victims. There is no definite treatment for respiratory disorders induced by SM exposure. However, the protective effect of Nigella sativa on inflammatory process was shown. In the present study, the protective effect of Nigella sativa on tracheal responsiveness and lung inflammation of SM exposed guinea pigs was examined. Guinea pigs were exposed to diluent's solution (ethanol, control group), 100 mg/m(3) inhaled sulfur mustard (SME group), and SME treated with Nigella sativa, 0.08 g daily (SME+N), n = 6 for each group. Tracheal responsiveness (TR) to methacholine, total white blood cell (WBC) count of lung lavage, and differential WBC were done 14 days post exposure. The weigh of animal were measured at the beginning, middle (day 7), and the end (day 14) of the study. The TR of SM-exposed guinea pigs was significantly (P<.001) and WBC nonsignificantly higher than those of controls. In SME guinea pigs, there was a weight loss but in the case of SME+N guinea pigs, no obvious weight change thought the study was seen. The eosinophl, monocyte, and lympocytes in SME animals were significantly changed compared to control group (P<.001 for all cases). Monocyte, lymphocyte, and neutrophil number were decreased in SME+N group compared to SME animals, which was significant only for neutrophil (P<.05). These results showed a preventive effect of Nigella sativa on TR of SM-exposed guinea pigs.

Article Published Date : May 01, 2008

Study Type : Animal Study

[63] Black cumin seed oil has significant protective activity against cytomegalovirus infection in the mouse experimental model

Abstract Title:

Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection.

Abstract Source:

Int J Immunopharmacol. 2000 Sep;22(9):729-40. PMID: 10884593

Abstract Author(s):

M L Salem, M S Hossain

Abstract:

In this study, antiviral effect of black seed oil (BSO) from Nigella sativa was investigated using murine cytomegalovirus (MCMV) as a model. The viral load and innate immunity mediated by NK cells and Mφ during early stage of the infection were analyzed. Intraperitoneal (i.p.) administration of BSO to BALB/c mice, a susceptible strain of MCMV infection, strikingly inhibited the virus titers in spleen and liver on day 3 of infection with 1x10(5) PFU MCMV. This effect coincided with an increase in serum level of IFN-gamma. Although BSO treatment decreased both number and cytolytic function of NK cells on day 3 of infection, it increased numbers of Mφ and CD4(+) T cells. On day 10 of infection, the virus titer was undetectable in spleen and liver of BSO-treated mice, while it was detectable in control mice. Although spleen of both control and BSO-treated mice showed similar CTL activities on day 10 after infection, serum level of IFN-gamma in BSO-treated mice was higher. Furthermore, BSO treatment upregulated suppressor function of Mφ in spleen. These results show that BSO exhibited a striking antiviral effect against MCMV infection which may be mediated by increasing of Mφ number and function, and IFN-gamma production.

Article Published Date : Sep 01, 2000

Study Type : Animal Study

[64] Black cumin's hyperglycemic activity in the rat model of diabetes may be due to beta cell regeneration/proliferation

Abstract Title:

Partial regeneration/proliferation of the beta-cells in the islets of Langerhans by Nigella sativa L. in streptozotocin-induced diabetic rats.

Abstract Source:

Tohoku J Exp Med. 2003 Dec;201(4):213-9. PMID: 14690013

Abstract Author(s):

Mehmet Kanter, Ismail Meral, Zabit Yener, Hanefi Ozbek, Halit Demir

Abstract:

This experiment was carried out to investigate the effect of N. sativa L. on histopathology of pancreatic beta-cells, and blood insulin and glucose concentrations in streptozotocin-induced diabetic rats. Fifty male Wistar rats (200-250 g) were divided into two experimental groups (diabetics with no treatment and diabetics with N. sativa L. treatment), each containing twenty-five rats. Diabetes was induced in both groups by a single intraperitoneal injection of streptozotocin (STZ) (50 mg/kg). The experimental animals in both groups became diabetic within 24 hours after the administration of STZ. The rats in N. sativa L.-treated group were given the daily intraperitoneal injection of 0.20 ml/kg of N. sativa L. volatile oil for 30 days starting the day after STZ injection. Control rats received only the same amount of normal saline solution. The rats in both groups received the last injection 24 hours before the sacrification and 5 randomly-selected rats in each group were sacrificed before, and the 1, 10, 20 and 30 days after the STZ injection to collect blood and pancreatic tissue samples. The N. sativa L. treatment caused a decrease in the elevated serum glucose, an increase in the lowered serum insulin concentrations and partial regeneration/ proliferation of pancreatic beta-cells in STZ-induced diabetic rats with the elapse of the experiment. It is concluded that the hypoglycaemic action of N. sativa L. could be partly due to amelioration in the beta-cells of pancreatic islets causing an increase in insulin secretion. More studies are needed to demonstrate the exact mechanism of action of N. sativa L. on ameliorated blood glucose concentration in STZ-induced diabetes.

Article Published Date : Dec 01, 2003

Study Type : Animal Study

[65] Black seed oil exhibited an anti-aging effect in a model of aging induced with D-galactose

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Abstract Title:

Anti-Aging Effect of Nigella Sativa Fixed Oil on D-Galactose-Induced Aging in Mice.

Abstract Source:

J Pharmacopuncture. 2017 Mar ;20(1):29-35. PMID: 28392960

Abstract Author(s):

Mahdieh Jafari Shahroudi, Soghra Mehri, Hossein Hosseinzadeh

Article Affiliation:

Mahdieh Jafari Shahroudi

Abstract:

OBJECTIVES: Aging is an unconscious and gradual process that can lead to changes in biological systems. Induction of oxidative stress and apoptosis, hepatotoxicity and neurotoxicity are involved in the aging process. Regarding the antioxidant property of black seed oil, the aim of this study was to evaluate the anti-aging effect of Nigella sativa (N. sativa) oil on d-galactose-induced aging in mice.

METHODS: For induction of aging, D-galactose (500 mg/kg, subcoutaneously SC) was administrated to male mice for 42 days. Animals were treated with D-galactose alone or with b lack seed oil (0.1, 0.2, 0.5 mL/kg, intraperitoneally (ip)). Additionally, vitamin E (200 mg/kg) was used as a positive control. At the end of treatment, the malondialdehyde (MDA) and the glutathione (GSH) contents in brain and liver tissues were measured. Also, enzymes in serum, including aspartate aminotransferase (AST) and alanine amino transferase (ALT), were determined. The levels of the proteins Bax, Bcl2, caspase-3 (pro and cleaved) in brain and liver tissues were evaluated.

RESULTS: Administration of D-galactose (500 mg/kg, SC) for 42 days increased serum levels of ALT and AST, as well as the MDA content, in brain and liver tissues, but decreased the GSH content. Additionally, the levels of apoptotic proteins, including Bax, procaspase-3 and caspase-3 cleaved, were markedly increased. N. sativa oil (0.1 and 0.2 mL/kg) diminished the levels of the biochemical markers ALT and AST. Administration of black seed oil (0.1, 0.2 and 0.5 mL/kg) reduced lipid peroxidation and at doses 0.1 and 0.2 mL/kg significantly recovered the GSH content. The oil decreased Bax/Bcl2 levels and at 0.1 mL/kg down-regulated the expressions of caspase-3 (pro and cleaved) proteins in brain and liver tissues.

CONCLUSION: Through its antioxidant and anti-apoptosis properties, black seed oil exhibited an anti-aging effect in a model of aging induced with D-galactose.

Article Published Date : Feb 28, 2017

Study Type : Animal Study

[66] Black seed oil has significant inhibitory effects against colon cancer in rats, without observable side effects

Abstract Title:

Chemopreventive potential of volatile oil from black cumin (Nigella sativa L.) seeds against rat colon carcinogenesis.

Abstract Source:

Nutr Cancer. 2003;45(2):195-202. PMID: 12881014

Abstract Author(s):

Elsayed I Salim, Shoji Fukushima

Abstract:

Chemopreventive effects of orally administered Nigella sativa oil on the induction and development of 1,2-dimethylhydrazine-induced aberrant crypt foci (ACF), putative preneoplastic lesions for colon cancer, were investigated in Fischer 344 rats. Starting at 6 wk of age, 45 male rats (groups 1-3) were subcutaneously injected with DMH once a week for 3 wk. Group 1 (15 rats) served as a carcinogen control group without N. sativa administration. Group 2 or 3 (15 rats each) were given the oil in the postinitiation stage or in the initiation stage, respectively. Animals of group 4 (11 rats) were injected with 0.9% saline and received N. sativa oil from the beginning until the termination. At sacrifice, 14 wk after the start, the total numbers of ACF as well as those with at least four crypts were significantly reduced in group 2 (P < 0.01). However, treatment with N. sativa oil in the initiation stage (group 3) did not exhibit significant inhibitory effects except on foci with only one aberrant crypt. Immunohistochemical analysis of 5-bromo-2'.-deoxyuridine labeling in colonic crypts revealed the N. sativa oil to have significant antiproliferative activity in both initiation and postinitiation stages and especially in the latter. Histological examination revealed no pathological changes in the liver, kidneys, spleen, or other organs of rats treated with N. sativa. In addition, biochemical parameters of blood and urine as well as body weight gain were not affected. These findings demonstrate that the volatile oil of N. sativa has the ability to inhibit colon carcinogenesis of rats in the postinitiation stage, with no evident adverse side effects, and that the inhibition may be associated, in part, with suppression of cell proliferation in the colonic mucosa.

Article Published Date : Jan 01, 2003

Study Type : Animal Study

[67] Both Nigella sativa and date reduce the toxic effects of aflatoxin-B1 in liver and kidney

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Abstract Title:

Study of protective effect of date and nigella sativa on aflatoxin b(1) toxicity.

Abstract Source:

Int J Health Sci (Qassim). 2008 Jul ;2(2):26-44. PMID: 21475486

Abstract Author(s):

Abdalla Al-Ghasham, Hesham Saad Ata, Said El-Deep, Abdel-Raheim Meki, Salah Shehada

Article Affiliation:

Abdalla Al-Ghasham

Abstract:

BACKGROUND: Many medicinal plants and their purified constituents have been shown beneficial therapeutic potentials. Seeds of Nigella sativa, a dicotyledon of the Ranunculaceae family, have been utilized for thousands of years as a spice and food preservative.

METHODS: the toxic effect of aflatoxin-B(1) (AFB(1)) and the possible cytoprotective effect of Nigella sativa (NS) oil and aqueous extract of date were studied on 40 male rats. The animals were divided into 4 groups (10 rats each) and treated daily for two weeks. Group 1 received normal saline as controls. Group 2 treated via intraperitoneal (IP) route with AFB(1) (50μg/kg BW). Group 3 treated with AFB(1) and NS oil via IP. Group 4 treated with AFB(1) and received orally aqueous extract of date (15mg/15ml). The liver and kidneys of each animal were histological examined and biochemical evaluation of the liver and kidney functions was performed.

RESULTS: Group 2 showed severe degenerative and necrotic changes in the liver and kidney. The plasma levels of alanine transaminase (ALT), aspartate transaminase (AST), creatinine and urea in AFB(1) group were significantly higher than the control group. Livers and kidneys of rats, treated with AFB(1) and NS showed less histopathological changes in comparison with the AFB(1) treated group. Livers and kidneys of rats treated with AFB1 and date group showed only mild histopathological changes in comparison with AFB(1) treated group. These histopathological changes seen in animals treated with AFB1 and dates were associated with a significant reduction in levels of ALT, AST, creatinine and urea. Likewise, histopathological changes in the AFB1 and NS group were associated with significant reduction in the levels of beforementioned indices. Moreover, AFB1 and date group showed significant improvement in liver function comparing with AFB(1) and NS group.

CONCLUSION: our study revealed that treatment with AFB(1) induced histopathological changes in the tissues of liver and kidney associated with dysfunction of these organs. Both NS and date reduce the toxic effects of AFB(1) in liver and kidney. But date treatment was more cytoprotective for liver than NS treatment against aflatoxicosis in rats.

Article Published Date : Jun 30, 2008

Study Type : Animal Study

[68] Both Nigella sativa and thymoquinone can partly protect gastric mucosa from acute alcohol-induced mucosal injury

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Abstract Title:

Gastroprotective activity of Nigella sativa L oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats.

Abstract Source:

World J Gastroenterol. 2005 Nov 14 ;11(42):6662-6. PMID: 16425361

Abstract Author(s):

Mehmet Kanter, Halit Demir, Cengiz Karakaya, Hanefi Ozbek

Article Affiliation:

Mehmet Kanter

Abstract:

AIM: To evaluate the role of reactive oxygen species in the pathogenesis of acute ethanol-induced gastric mucosal lesions and the effect of Nigella sativa L oil (NS) and its constituent thymoquinone (TQ) in an experimental model.

METHODS: Male Wistar albino rats were assigned into 4 groups. Control group was given physiologic saline orally (10 mL/kg body weight) as the vehicle (gavage); ethanol group was administrated 1 mL (per rat) absolute alcohol by gavage; the third and fourth groups were given NS (10 mL/kg body weight) and TQ (10 mg/kg body weight p.o) respectively 1 h prior to alcohol intake. One hour after ethanol administration, stomach tissues were excised for macroscopic examination and biochemical analysis.

RESULTS: NS and TQ could protect gastric mucosa against the injurious effect of absolute alcohol and promote ulcer healing as evidenced from the ulcer index (UI) values. NS prevented alcohol-induced increase in thiobarbituric acid-reactive substances (TBARS), an index of lipid peroxidation. NS also increased gastric glutathione content (GSH), enzymatic activities of gastric superoxide dismutase (SOD) and glutathione-S-transferase (GST). Likewise, TQ protected against the ulcerating effect of alcohol and mitigated most of the biochemical adverse effects induced by alcohol in gastric mucosa, but to a lesser extent than NS. Neither NS nor TQ affected catalase activity in gastric tissue.

CONCLUSION: Both NS and TQ, particularly NS can partly protect gastric mucosa from acute alcohol-induced mucosal injury, and these gastroprotective effects might be induced, at least partly by their radical scavenging activity.

Article Published Date : Nov 13, 2005

Study Type : Animal Study

[69] Both Nigella sativa fixed and essential oil are effective in improving the antioxidant indices against potassium bromate induced oxidative stress

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Abstract Title:

Nigella sativa fixed and essential oil modulates glutathione redox enzymes in potassium bromate induced oxidative stress.

Abstract Source:

BMC Complement Altern Med. 2015 ;15(1):330. Epub 2015 Sep 18. PMID: 26385559

Abstract Author(s):

Muhammad Tauseef Sultan, Masood Sadiq Butt, Roselina Karim, Waqas Ahmed, Ubedullah Kaka, Shakeel Ahmad, Saikat Dewanjee, Hawa Ze Jaafar, M Zia-Ul-Haq

Article Affiliation:

Muhammad Tauseef Sultan

Abstract:

BACKGROUND: Nigella sativa is an important component of several traditional herbal preparations in various countries. It finds its applications in improving overall health and boosting immunity. The current study evaluated the role of fixed and essential oil of Nigella sativa against potassium bromate induced oxidative stress with special reference to modulation of glutathione redox enzymes and myeloperoxidase.

METHODS: Animals; 30 rats (Sprague Dawley) were divided in three groups and oxidative stress was induced using mild dose of potassium bromate. The groups were on their respective diets (iso-caloric diets for a period of 56 days) i.e. control and two experimental diets containing N. sativa fixed (4 %) and essential (0.3 %) oils. The activities of enzymes involved in glutathione redox system and myeloperoxidase (MPO) were analyzed.

RESULTS: The experimental diets modulated the activities of enzymes i.e. glutathione-S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx) positively. Indices of antioxidant status like tocopherols and glutathione were in linear relationship with that of GPx, GR and GST (P < 0.01). MPO activities were in negative correlation with GST (P < 0.01) but positive correlation with some other parameters.

CONCLUSIONS: Our results indicated that both Nigella sativa fixed and essential oil are effective in improving the antioxidant indices against potassium bromate induced oxidative stress.

Article Published Date : Dec 31, 2014

Study Type : Animal Study

[70] Both Nigella sativa oil and thymoquinone possess gastroprotective effect against gastric lesions

Abstract Title:

Gastroprotective activity of Nigella sativa oil and its constituent, thymoquinone, against gastric mucosal injury induced by ischaemia/reperfusion in rats.

Abstract Source:

J Ethnopharmacol. 2003 Feb ;84(2-3):251-8. PMID: 12648823

Abstract Author(s):

H S El-Abhar, D M Abdallah, S Saleh

Article Affiliation:

H S El-Abhar

Abstract:

Ischaemia/reperfusion (I/R) induced gastric lesion, is known to be linked with free radical (FR) formation. Therefore, this model was used to assess the antioxidant effects of Nigella sativa oil (N.O) and thymoquinone (TQ) on gastric mucosal redox state and gastric lesions, 1 and 24 h after reperfusion. Male Wistar rats were subjected to I/R and were injected with either N.O (2.5 and 5 ml/kg, p.o) or TQ (5, 20, 50 and 100 mg/kg, p.o). The results showed that I/R elevated the levels of lipid peroxide (LPX) and lactate dehydrogenase (LDH), while decreased those of reduced glutathione (GSH) and superoxide dismutase (SOD). These biochemical changes were accompanied by an increase in the formation of gastric lesions, which was reduced by either treatment. N.O tended to normalize the level of LDH, GSH and SOD. However, its effect to restore LPX was only seen 24 h after reperfusion. Moreover, the aforementioned parameters were nearly reinstated by TQ. On the other hand, high doses of TQ (50 and 100 mg/kg) severely reduced the GSH content, 1 h after reperfusion. These results indicate that both N.O and TQ possess gastroprotective effect against gastric lesions which may be related to the conservation of the gastric mucosal redox state.

Article Published Date : Jan 31, 2003

Study Type : Animal Study

[71] Chronic administration of N. sativa seed extract has a significant hypoglycemic effect

Abstract Title:

Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta.

Abstract Source:

Avicenna J Phytomed. 2016 Jan-Feb;6(1):67-76. PMID: 27247923

Abstract Author(s):

Abbasali Abbasnezhad, Saeed Niazmand, Maryam Mahmoudabady, Mohammad Soukhtanloo, Seyed Abdolrahim Rezaee, Seyed Mojtaba Mousavi

Article Affiliation:

Abbasali Abbasnezhad

Abstract:

OBJECTIVE: Diabetes is an important risk factor for cardiovascular events. The great percent of morbidity in patients with diabetes is due to endothelial dysfunction. The present study investigated the effects of hydroalcholic extract of Nigella sativa (N. sativa) on contractile and dilatation response of isolated aorta in streptozotocin (STZ)-induced diabetic rat.

MATERIALS AND METHODS: Rats were divided into six experimental groups (control, untreated STZ-diabetic, and N. sativa hydroalcholic extract or metformin-treated diabetic rats). Treated rats received N. sativa extract (100, 200, and 400 mg/kg) or metformin (300 mg/kg) by gavage, daily for 6 weeks. Isolated rat thoracic rings were mounted in an organ bath system then contractile and dilatation responses induced by phenylephrine (PE), acetylcholine (ACh), potassium chloride (KCl), and sodium nitroprusside (SNP) were evaluated in different situations.

RESULTS: The lower concentrations of N. sativa seed extract (DE 100 and DE 200) and metformin significantly reduced the contractile responses to higher concentrations of PE (10(-6) - 10(-5) M) compared to diabetic group (p<0.05 to p<0.01). The relaxation response to Ach 10(-8) M, was increased in DE 200 and metformin groups compared to diabetic group (p<0.05). The relaxation responses to Ach 10(-7) - 10(-5) M were significantly higher in all treated groups compared to diabetic group (p<0.05 to p<0.001).

CONCLUSION: Chronic administration of N. sativa seed extract has a significant hypoglycemic effect and improves aortic reactivity to vasoconstrictor and vasodilator agents in STZ-induced diabetic rats.

Article Published Date : Dec 31, 2015

Study Type : Animal Study

[72] Covering peritoneal surfaces with nigella sativa oil after peritoneal trauma is effective in decreasing peritoneal adhesion formation

Abstract Title:

Effect of Nigella sativa oil on postoperative peritoneal adhesion formation.

Abstract Source:

J Obstet Gynaecol Res. 2013 Oct 7. doi: 10.1111/jog.12172.

Abstract Author(s):

Ahmet Sahbaz, Firat Ersan, Serdar Aydin

Article Affiliation:

School of Medicine, Department of Obstetrics and Gynecology, Bulent Ecevit University, Kozlu/Zonguldak, Turkey.

Abstract:

AIM: We aim to evaluate the effect of Nigella sativa oil (NSO) on postoperative peritoneal adhesion formation in female rats. This experimental study is the first on the prevention of postoperative adhesion formation by NSO.

METHODS: Twenty-four Wistar albino female rats were randomly assigned to three groups of eight rats each. Rats in group 1 were each injected i.p. with 1 mL of NSO. In group 2, an adhesion model was created with no injection of NSO. In group 3, an adhesion model was created and the area was covered with 1 mL of NSO. The rats were killed on postoperative day 8, and the severity of adhesions was evaluated macroscopically and histopathologically.

RESULTS: There was a statistically significant difference in adhesion scores between group 2 (control) and group 3 (NSO-treated) (P = 0.003). Statistically significant differences in angiogenesis, fibrosis and inflammation were observed between the control and Nigella sativa groups (P = 0.002, P = 0.001 and P = 0.004, respectively).

CONCLUSION: Covering peritoneal surfaces with NSO after peritoneal trauma is effective in decreasing peritoneal adhesion formation.

Article Published Date : Oct 06, 2013

Study Type : Animal Study

[73] Effects of Nigella sativa and human parathyroid hormone on bone mass and strength in diabetic rats

Abstract Title:

Effects of Nigella sativa and human parathyroid hormone on bone mass and strength in diabetic rats.

Abstract Source:

Biol Trace Elem Res. 2007 Jun ;116(3):321-8. PMID: 17709912

Abstract Author(s):

Mehmet Fatih Altan

Article Affiliation:

Mehmet Fatih Altan

Abstract:

Osteoporosis is a major complication in patients with diabetes mellitus (DM), particularly in those with insulin dependency. Recently, many therapeutic effects of Nigella sativa L. (NS) extracts have been exhibited such as anti-inflammatory, antitumor, and antidiabetic with clinical and experimental studies. Mechanical strength in the femur and vertebrae increases with human parathyroid hormone (hPTH) treatment. The aim of the present study was to test the hypothesis that combined treatment with NS and hPTH is more effective than treatment with NS or hPTH alone in improving bone mass, connectivity, and biomechanical behavior using the finite element method (FEM) in insulin-dependent diabetic rats. In the mechanical analysis, five rat bones (control, diabetic diabetic NS treated, diabetic hPTH treated, and diabetic NS + hPTH treated) have been studied for bending analysis using the finite element analysis program ANSYS. Combined treatment of NS and hPTH was more effective on bone histomorphometry and mechanical strength than treatment with NS or hPTH alone for streptozotocin-induced diabetic osteopenia, which notably decreased bone volume.

Article Published Date : May 31, 2007

Study Type : Animal Study

[74] Ethanolic extract of Nigella sativa possessed significant analgesic effect in mice

Abstract Title:

Analgesic effect of Nigella sativa seeds extract on experimentally induced pain in albino mice.

Abstract Source:

J Coll Physicians Surg Pak. 2010 Jul ;20(7):464-7. PMID: 20642947

Abstract Author(s):

Muhammad Usman Bashir, Hamid Javaid Qureshi

Article Affiliation:

Muhammad Usman Bashir

Abstract:

OBJECTIVE: To determine the analgesic effect of ethanolic extract of Nigella sativa seeds on experimentally-induced pain in albino mice.

STUDY DESIGN: Randomized controlled trial (RCT).

PLACE AND DURATION OF STUDY: Physiology Department, Services Institute of Medical Sciences (SIMS), Lahore, from May to September, 2009.

METHODOLOGY: The study was carried out in 90 male albino mice using acetic acid induced writhing test as a chemical model of nociception. The mice were divided in three groups of 30 each. Group A was given normal saline (control); group B was given Nigella sativa seed extract in a dose of 50 mg/kg; and group C received diclofenac sodium, as a reference drug. Number of writhings in treated and control groups were compared.

RESULTS: The ethanolic extract of Nigella sativa seeds given intraperitoneally caused significant (p<0.05) analgesic effect on nociceptive response initiated by 0.6% acetic acid; although this analgesic effect was less than that produced by diclofenac sodium.

CONCLUSION: Ethanolic extract of Nigella sativa possessed significant analgesic effect in mice.

Article Published Date : Jun 30, 2010

Study Type : Animal Study

[75] Feeding of Nigella sativa during neonatal and juvenile growth improves learning and memory of rats

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Abstract Title:

Feeding of Nigella sativa during neonatal and juvenile growth improves learning and memory of rats.

Abstract Source:

J Tradit Complement Med. 2016 Apr ;6(2):146-52. Epub 2015 Jan 17. PMID: 27114937

Abstract Author(s):

Farimah Beheshti, Mahmoud Hosseini, Farzaneh Vafaee, Mohammad Naser Shafei, Mohammad Soukhtanloo

Article Affiliation:

Farimah Beheshti

Abstract:

The positive roles of antioxidants on brain development and learning and memory have been suggested. Nigella sativa (NS) has been suggested to have antioxidant and neuroprotective effects. This study was done to investigate the effects of feeding by the hydro-alcoholic extract of NS during neonatal and juvenile growth on learning and memory of rats. The pregnant rats were kept in separate cages. After delivery, they were randomly divided into four Groups including: (1) control; (2) NS 100 mg/kg (NS 100); (3) NS 200 mg/kg (NS 200); and (4) NS 400 mg/kg (NS 400). Rats in the control group (Group 1) received normal drinking water, whereas Groups 2, 3, and 4 received the same drinking water supplemented with the hydro-alcoholic extract of NS (100 mg/kg, 200 mg/kg, and 400 mg/kg, respectively) from the 1st day after birth through the first 8 weeks of life. After 8 weeks, 10 male offspring from each group were randomly selected and tested in the Morris water maze (MWM) and passive avoidance (PA) test. Finally, the brains were removed and total thiol groups and malondialdehyde(MDA) concentrations were determined. In the MWM, treatment by 400 mg/kg extract reduced both the time latency and the distance traveled to reach the platform compared to the control group (p < 0.05-p < 0.01). Both 200 mg/kg and 400 mg/kg of the extract increased the time spent in the target quadrant (p < 0.05-p < 0.01). In the PA test, the treatment of the animals by 200 mg/kg and 400 mg/kg of NS extract significantly increased the time latency for entering the dark compartment (p < 0.05-p < 0.001). Pretreatment of the animals with 400 mg/kg of NS extract decreased the MDA concentration in hippocampal tissues whereas it increased the thiol content compared to the control group (p < 0.001). These results allow us to propose that feeding of the rats by the hydro-alcoholic extract of NS during neonatal and juvenile growth has positive effects on learning and memory. The effects might be due to the antioxidant effects.

Article Published Date : Mar 31, 2016

Study Type : Animal Study

[76] Fish oil and Nigella sative volatile oil emulsion had promising hepato-regenerative and reno-protective abilities

Abstract Title:

Hepatic Regeneration and Reno-Protection by Fish oil, Nigella sativa Oil and Combined Fish Oil/Nigella sativa Volatiles in CClTreated Rats.

Abstract Source:

J Oleo Sci. 2018 Feb 19. Epub 2018 Feb 19. PMID: 29459508

Abstract Author(s):

Sahar Y Al-Okbi, Doha A Mohamed, Thanaa E Hamed, Amr E Edris, Karem Fouda

Article Affiliation:

Sahar Y Al-Okbi

Abstract:

The aim of the present research was to investigate the effect of fish oil, crude Nigella sative oil and combined fish oil/Nigella sative volatile oil as hepato-regenerative and renal protective supplements. The oils were administered as emulsions to rat model with liver injury induced by CCl. Plasma activities of transaminases (AST and ALT) were evaluated as liver function indicators, while plasma creatinine and urea and creatinine clearance were determined as markers of kidney function. Plasma malondialdehyde (MDA), nitrite (NO) and tumor necrosis factor-α (TNF-α) were estimated to assess the exposure to oxidative stress and subsequent inflammation. Liver fat was extracted and their fatty acids´ methyl esters were determined using gas chromatography. Results showed that plasma activities of AST and ALT were significantly higher in CClcontrol group compared to control healthy group. Plasma levels of creatinine and urea increased significantly in CClcontrol, while creatinine clearance was reduced significantly in the same group. All rat treated groups given the three oil emulsions showed improvement in liver function pointing to the initiation of liver regeneration. The combination of fish oil/Nigella sative volatiles showed the most promising regenerative activity. Oxidative stress and inflammation which were increased significantly in CClcontrol group showed improvement on administration of the three different oil emulsions. Fatty acids methyl ester of liver fat revealed that rats treated with fish oil/Nigella sative volatile oil presented the highest content of unsaturated fatty acids (45.52%± 0.81) while fish oil showed the highest saturated fatty acids (53.28% ± 1.68). Conclusion; Oral administration of oil emulsions of native fish oil, Nigella sative crude oil and combined fish oil/Nigella sative volatile oil reduced liver and kidney injury in rat model of CClthrough exerting anti-inflammatory and antioxidant activity. Fish oil/Nigella sative volatile oil emulsion was the most promising hepato-regenerative and reno-protective formula among the different groups.

Article Published Date : Feb 18, 2018

Study Type : Animal Study

[77] Garlic extract and Nigella sativa oil are promising agents to complement schistosomiasis specific treatment

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Abstract Title:

The effect of antioxidant properties of aqueous garlic extract and Nigella sativa as anti-schistosomiasis agents in mice.

Abstract Source:

Rev Inst Med Trop Sao Paulo. 2008 Jan-Feb;50(1):29-36. PMID: 18327484

Abstract Author(s):

Nahla S El Shenawy, Maha F M Soliman, Shimaa I Reyad

Article Affiliation:

Nahla S El Shenawy

Abstract:

The aim of this study was to assess the antioxidant and anti-schistosomal activities of the garlic extract (AGE) and Nigella sativa oil (NSO) on normal and Schistosoma mansoni-infected mice. AGE (125 mg kg-1, i.p.) and NSO (0.2 mg kg-1, i.p.) were administrated separately or in combination for successive 28 days, starting from the 1st day post infection (pi). All mice were sacrificed at weeks 7 pi. Hematological and biochemical parameters including liver and kidney functions were measured to assess the progress of anemia, and the possibility of the tissue damage. Serum total protein level, albumin, globulin and cholesterol were also determined. Malondialdehyde (MDA) and glutathione (GSH) levels were determined in the liver tissues as biomarkers for oxidative and reducing status, respectively. The possible effect of the treatment regimens on Schistosoma worms was evaluated by recording percentage of the recovered worms, tissue egg and oogram pattern. Result showed that, protection with AGE and NSO prevented most of the hematological and biochemical changes and markedly improved the antioxidant capacity of schistosomiasis mice compared to the infected-untreated ones. In addition, remarkable reduction in worms, tissue eggs and alteration in oogram pattern were recorded in all the treated groups. The antioxidant and antischistosomal action of AGE and NSO was greatly diverse according to treatment regimens. These data point to these compounds as promising agents to complement schistosomiasis specific treatment.

Article Published Date : Dec 31, 2007

Study Type : Animal Study

[78] Grape seed extract, black seed extract and curcumin ameliorate tamoxifen-induced liver injury

Abstract Title:

Amelioration of tamoxifen-induced liver injury in rats by grape seed extract, black seed extract and curcumin.

Abstract Source:

Hepatogastroenterology. 2007 Jan-Feb;54(73):320-4. PMID: 21046982

Abstract Author(s):

Hesham A El-Beshbishy, Ahmed M Mohamadin, Ayman A Nagy, Ashraf B Abdel-Naim

Article Affiliation:

Medical Laboratories Technology Dept., Facuty of Applied Medical Sciences, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia. hesham_elbeshbishy@hotmail.com

Abstract:

Liver injury was induced in female rats using tamoxifen (TAM). Grape seeds (Vitis vinifera) extract (GSE), black seed (Nigella sativa) extract (NSE), curcumin (CUR) or silymarin (SYL) were orally administered to TAM-intoxicated rats. Liver histopathology of TAM-intoxicated:rats showed pathological changes. TAM-intoxication elicited declines in liver antioxidant enzymes levels (glutathione peroxidase, glutathione reductase, superoxide dismutase and catalase), reduced glutathione (GSH) and GSH/GSSG ratio plus the hepatic elevations in lipid peroxides, oxidized glutathione (GSSG), tumor necrosis factor-alpha (TNF-alpha) and serum liver enzymes; alanine transaminase, aspartate transaminase, alkaline phosphatase, lactate dehydrogenase and gamma glutamyl transferase levels. Oral intake of NSE, GSE, CUR or SYL to TAM-intoxicated rats, attenuated histopathological changes and corrected all parameters mentioned above. Improvements were prominent in case of NSE (similarly SYL)>CUR>GSE. Data indicated that NSE, GSE or CUR act as free radicals scavengers and protect TAM-induced liver injury in rats.

Article Published Date : Jan 01, 2007

Study Type : Animal Study

[79] Hydro-alcoholic extracts of N.sativa and C.longa led to an improvement in ADR-induced oxidative stress

Abstract Title:

Adriamycin-induced oxidative stress is prevented by mixed hydro-alcoholic extract of Nigella sativa and Curcuma longa in rat kidney.

Abstract Source:

Avicenna J Phytomed. 2016 Jan-Feb;6(1):86-94. PMID: 27247925

Abstract Author(s):

Reza Mohebbati, Mohammad Naser Shafei, Mohammad Soukhtanloo, Noema Mohammadian Roshan, Abolfazl Khajavi Rad, Akbar Anaeigoudari, Sara Hosseinian, Sareh Karimi, Farimah Beheshti

Article Affiliation:

Reza Mohebbati

Abstract:

OBJECTIVE: Inflammation and oxidative stress is considered to have a crucial role in induction of nephropathy. Curcuma longa (C. longa) and Nigella sativa (N. sativa) have anti-inflammatory and antioxidant effects. This study was designed to investigate the effect of mixed hydro-alcoholic extract of N.sativa and C. longa on the oxidative stress induced by Adriamycin (ADR) in rat kidney.

MATERIALS AND METHODS: The animals were divided into 6 groups: control (CO), ADR, Adriamycin+ Vitamin C (ADR+VIT C), C. longa extract+ Adriamycin (C.LE+ADR), N. sativa extract+ Adriamycin (N.SE+ADR) and C. longa extract+ N. sativa extract + Adriamycin (N.S+C.L+ADR). ADR (5mg/kg) was injected intravenously, whereas VITC (100mg/kg) and extract of C. longa (1000mg/kg) and N. sativa (200mg/kg) were administrated orally. Finally, the renal tissue, urine and blood samples were collected and submitted to measure of redox markers, osmolarity and renal index.

RESULTS: The renal content of total thiol and superoxide dismutase (SOD) activity significantly decreased and Malondialdehyde (MDA) concentration increased in Adriamycin group compared to control group. The renal content of total thiol and SOD activity significantly enhanced and MDA concentration reduced in treated-mixed extract of C. longa and N. sativa along with ADR group compared to ADR group. The mixed extract did not restore increased renal index percentage induced by ADR. There also was no significant difference in urine and serum osmolarity between the groups.

CONCLUSION: hydro-alcoholic extracts of N.sativa and C.longa led to an improvement in ADR-induced oxidative stress and mixed administration of the extracts enhanced the aforementioned therapeutic effect.

Article Published Date : Dec 31, 2015

Study Type : Animal Study

[80] In vivo attenuation of angiogenesis in hepatocellular carcinoma by Nigella sativa

Abstract Title:

In vivo attenuation of angiogenesis in hepatocellular carcinoma by Nigella sativa

Abstract Source:

Turk J Med Sci. 2018 Feb 23 ;48(1):178-186. Epub 2018 Feb 23. PMID: 29479981

Abstract Author(s):

Moustafa Fathy, Toshio Nikaido

Article Affiliation:

Moustafa Fathy

Abstract:

Background/aim: Angiogenesis is imperative in malignant tumor growth. Hepatocellular carcinoma is a typical hypervascular tumor that relies on angiogenesis. The aim of this study is to investigate the in vivo molecular mechanism underlying the antitumor properties of Nigella sativa ethanolic extract (NSEE) through its antiangiogenic effect against diethyl nitrosamine (DENA)-induced hepatocarcinogenesis. Materials and methods: Male Wistar rats were divided into 4 groups: normal, NSEE, DENA, and NSEE-DENA groups. Final body weight, hepatosomatic indices, serum AFP, serum vascular endothelial growth factor (VEGF) levels, and liver tissue hepatocyte growth factorß (HGFß) protein expression were estimated. Liver sections were stained for histological examination. AFP levels with the histological variations were chosen to confirm cancer development. Results: DENA significantly increased liver weight, hepatosomatic indices, serum AFP and VEGF levels, and liver HGFß protein expression and significantly decreased final body weight. These effects were significantly reversed by NSEE. Furthermore, the histopathological changes that appeared in rat livers due to DENA were reduced by NSEE without harmful effects when taken alone. Conclusion: The results of the present investigation provide evidence that the in vivo antiangiogenic effect of NSEE through downregulation of serum VEGF and liver HGFß protein could be implicated in its antitumor activity. Its consumption has health benefits that favor liver cancer management. These findings might prove useful and helpful for the progression of therapies for hepatocarcinogenesis treatment.

Article Published Date : Feb 22, 2018

Study Type : Animal Study

[81] Intratympanic nigella sativa oil protects against gentamicin induced hearing loss

Abstract Title:

Protective role of intratympanic nigella sativa oil against gentamicin induced hearing loss.

Abstract Source:

Int J Pediatr Otorhinolaryngol. 2017 Jun ;97:83-88. Epub 2017 Mar 30. PMID: 28483257

Abstract Author(s):

Deniz Tuna Edizer, Ozgur Yigit, Zehra Cinar, Mehmet Gul, Eyyup Kara, Birgul Yigitcan, Duygu Hayır, Ahmet Atas

Article Affiliation:

Deniz Tuna Edizer

Abstract:

OBJECTIVE: Aminoglycosides, used to combat with life-threatening infections, have a substantial risk of hearing loss. Nigella sativa is an annual herbaceous plant and used for treatment of many diseases for ages. We aimed to investigate the protective role of intratympanic nigella sativa oil against gentamicin induced hearing loss in an animal model.

METHODS AND MATERIALS: Twenty eight guinea pigs were randomly divided into four groups: i-control, ii- Intratympanic nigella sativa oil (IT-NSO), iii- Intraperitoneal gentamicin (IP-G) and iv- Intraperitoneal gentamicin and intratympanic nigella sativa oil (IP-G + IT-NSO). Preoperative and postoperative hearing thresholds were determined with auditory brainstem response with click and 8 kHz tone-burst stimuli. Histological analysis of the cochlea specimens were performed under light microscope. Semiquantitative grading of the histological findings was carried out and compared between the groups.

RESULTS: Highest posttreatment hearing thresholds were detected in IP-G group. Posttreatment mean hearing threshold of the IP-G group with click stimulus was significantly higher than the IP-G + IT-NSO group (p = 0.004). whereas the difference was not significant with 8 kHz tone-burst stimulus (p = 0.137). Both IP-G and IP-G + IT-NSO groups had significantly higher hearing thresholds compared to control and IT-NSO groups (p > 0.05). Histological examination of the control and IT-NSO groups demonstrated normal appearance of cochlear nerve, stria vascularis and organ of Corti. IP-G group showed the most severe histological alterations including hydropic and vacuolar degenerations, hair cell damage and deformation of thebasilar mambrane. Histological evidence of damage was significantly reduced in IP-G + IT-NSO group compared to IP-G group.

CONCLUSION: Addition of intratympanic NSO to systemic gentamicin was demonstrated to have beneficial effects in hearing thresholds which was supported by histological findings.

Article Published Date : May 31, 2017

Study Type : Animal Study

[82] Memory enhancing effect of Nigella Sativa hydro-alcoholic extract on lipopolysaccharide-induced memory impairment

Abstract Title:

Memory enhancing effect of Nigella Sativa hydro-alcoholic extract on lipopolysaccharide-induced memory impairment in rats.

Abstract Source:

Drug Chem Toxicol. 2018 Mar 28:1-10. Epub 2018 Mar 28. PMID: 29589766

Abstract Author(s):

Fatemeh Norouzi, Mahmoud Hosseini, Azam Abareshi, Farimah Beheshti, Majid Khazaei, Mohammad Naser Shafei, Mohammad Soukhtanloo, Zahra Gholamnezhad, Akbar Anaeigoudari

Article Affiliation:

Fatemeh Norouzi

Abstract:

In this study, the effects of Nigella Sativa (NS) hydro-alcoholic extract on lipopolysaccharide (LPS)-induced learning and memory impairments, hippocampal cytokine levels, and brain tissues oxidative damage were investigated in rats. The rats were grouped and treated: (1) control (saline), (2) LPS (1 mg/kg i.p.), and (3-5) 100, 200, or 400 mg/kg NS hydro-alcoholic extract 30 min before LPS injection. The treatment was started since 6 days before the behavioral experiments and continued during the behavioral tests (LPS injection 2 h before each behavioral experiment). Finally, the brains were removed for biochemical assessments. In Morris water maze (MWM) test, LPS increased the escape latency and traveled path compared to control group, whereas all doses of NS hydro-alcoholic extract decreased them compared to LPS group. In passive avoidance (PA) test, the latency to enter the dark compartment in LPS group was shorter than control group while in all treated groups it was longer than LPS group. LPS increased tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), malondialdehyde (MDA), and nitric oxide (NO) metabolites, and decreased thiol content, superoxide dismutase (SOD), and catalase (CAT) in the hippocampal tissues compared to control group while NS hydro-alcoholic extract decreased MDA and NO metabolites and increased thiol content, SOD, and CAT compared to LPS group. Findings of the current study indicated that the hydro-alcoholic extract of NS improved the LPS-induced learning and memory impairments induced by LPS in rats by improving hippocampal cytokine levels and brain tissues oxidative damage.

Article Published Date : Mar 27, 2018

Study Type : Animal Study

[83] Methanolic extracts and volatile oil extracts of N. sativa seed oil may be used as antioxidant as well as hypolipidemic agents

Abstract Title:

Evaluation of therapeutic effect of omega-6 linoleic acid and thymoquinone enriched extracts from Nigella sativa oil in the mitigation of lipidemic oxidative stress in rats.

Abstract Source:

Nutrition. 2015 Dec 18. Epub 2015 Dec 18. PMID: 26827238

Abstract Author(s):

Shafeeque Ahmad, Zafarul H Beg

Article Affiliation:

Shafeeque Ahmad

Abstract:

OBJECTIVE: Nigella sativa belongs to the Ranunculaceae family. The therapeutic role of methanolic extract (ME) and volatile oil (VO) fractionated from N. sativa seed oil was investigated for antiperoxidative and antioxidant effects in atherogenic suspension fed rats.

METHODS: We examined the protective effects of ME and VO on the enzymatic and nonenzymatic antioxidants status in erythrocytes and the livers of atherogenic suspension fed rats. As a marker of lipid peroxidation, we estimated the conjugated diene, lipid hydroperoxide, and malondialdehyde concentrations in plasma in the following groups of rats: normolipidemic control, hyperlipidemic control, hyperlipidemic methanolic extract, and hyperlipidemic volatile oil. ME 500 mg or VO 100 mg/kg body weight of male rat was orally administrated for 30 d.

RESULTS: Pretreatment of hyperlipidemic rats with these test extracts resulted in a significant decrease (P < 0.001) in the level of lipid peroxidation markers, conjugated diene, lipid hydroperoxide, and malondialdehyde (16-50%) compared to the hyperlipidemic control rats. In addition, ME and VO significantly (P < 0.001) elevated the hepatic and erythrocyte superoxide dismutase, catalase, and glutathione reductase activities (19-58%) compared to the hyperlipidemic rats. In liver homogenate of hyperlipidemic-ME and hyperlipidemic-VO, the glutathione-S-transferase activity was protected by 93% and 89%, and inerythrocytes, the glutathione peroxidase activity was protected by 90% and 77%, respectively. Interestingly, reduced glutathione level and activities of ATPases were protected to near normal levels. Pretreatment of rats with the test extracts replenished effectively (P < 0.001) the plasma total antioxidant power by an average of 88% against free radicals.

CONCLUSIONS: The lipidemic oxidative stress was effectively mitigated by antiperoxidative activities of ME and VO. Thus, these test extracts, especially ME, may be used as antioxidant as well as hypolipidemic agents in the form of natural food supplement to prevent or treat diseases caused by free radicals.

Article Published Date : Dec 17, 2015

Study Type : Animal Study

[84] N sativa may protect brain and medulla spinalis tissues against oxidative stress induced by experimental autoimmune

Abstract Title:

The effect of Nigella sativa oil against experimental allergic encephalomyelitis via nitric oxide and other oxidative stress parameters.

Abstract Source:

Cell Mol Biol (Noisy-le-grand). 2005 Sep 5 ;51(3):337-42. Epub 2005 Sep 5. PMID: 16191402

Abstract Author(s):

F Ozugurlu, S Sahin, N Idiz, O Akyol, A Ilhan, R Yigitoglu, B Isik

Article Affiliation:

F Ozugurlu

Abstract:

Reactive oxygen species (ROS) including nitric oxide (NO) are thought to be involved in inflammatory processes, exacerbating inflammation and tissue damage in multiple sclerosis (MS). The oil extracts of Nigella sativa (N. sativa) has been known as an antioxidant and antiinflammatory agent. The aim of the present study was to investigate the hypothesis that N. sativa components provide protection against oxidative stress induced by experimental autoimmune encephalomyelitis (EAE) in rats. For this purpose, EAE was induced in rats by using guinea pig myelin basic protein (MBP) in Freud's adjuvant with addition of heat-killed M. Tuberculosis H37Ra to test this hypothesis. In study groups, N. sativa was given by oral gavage to the rats. Treatment of the rats with N. sativa inhibited ROS production induced by EAE showing diminished levels of MDA of both brain and medulla spinalis tissues. Although there was a significant decrease in brain NO level, there was an increase in medulla spinalis NO level after EAE induction in rats. N. sativa regulated tissue NO levels in some extend when applied together with EAE. When N. sativa was given alone to the rats, no changes were shown in brain, medulla spinalis, and serum oxidant/antioxidant parameters. In conclusion, N sativa may protect brain and medulla spinalis tissues against oxidative stress induced by EAE. In addition, N. sativa display its antioxidant and regulatory effects via inflammatory cells rather than the host tissue (brain and medulla spinalis) for EAE in rats.

Article Published Date : Sep 04, 2005

Study Type : Animal Study

[85] N. sativa administration exerts potent inhibitory effects on rat tumor development and on cellular proliferation in multiple organ sites

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Abstract Title:

Cancer chemopreventive potential of volatile oil from black cumin seeds, Nigella sativa L., in a rat multi-organ carcinogenesis bioassay.

Abstract Source:

Oncol Lett. 2010 Sep ;1(5):913-924. Epub 2010 Sep 1. PMID: 22966405

Abstract Author(s):

Elsayed I Salim

Article Affiliation:

Elsayed I Salim

Abstract:

Nigella sativa (N. sativa) is a herbal plant of the Ranunculaceae family that has been widely used for various medicinal and nutritional purposes. Volatile oil extracts along with its major constituents, such as thymoquinone, have recently attracted considerable attention for their antioxidant, immunoprotective and antitumor properties. The present study was conducted to assess the chemopreventive potential of crude oils in N. sativa on tumor formation using a well-established rat multi-organ carcinogenesis model featuring initial treatment with five different carcinogens. Post-initiation administration of 1000 or 4000 ppm N. sativa volatile oil in the diet of male Wistar rats for 30 weeks significantly reduced malignant and benign colon tumor sizes, incidences and multiplicities. The treatment also significantly decreased the incidences and multiplicities of tumors in the lungs and in different parts of the alimentary canal, particularly the esophagus and forestomach. Bromodeoxyuridine labeling indices, reflecting cell proliferation were significantly decreased in various organs and lesions after treatment with the two doses of N. sativa. The plasma levels of insulin growth factor, triglycerides and prostaglandin E2 were also altered. The findings show, for the first time, that N. sativa administration exerts potent inhibitory effects on rat tumor development and on cellular proliferation in multiple organ sites. In particular, the ability to significantly inhibit murine colon, lung, esophageal and forestomach tumors was demonstrated in the post-initiation phase, with no evidence of clinical side effects. The mechanisms are likely to be related to suppression of cell proliferation.

Article Published Date : Aug 31, 2010

Study Type : Animal Study

[86] N. sativa and propolis could be excellent adjuvant support in the therapy of diabetes mellitus and preventing its complications

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Abstract Title:

The Antidiabetic Activity of Nigella sativa and Propolis on Streptozotocin-Induced Diabetes and Diabetic Nephropathy in Male Rats.

Abstract Source:

Evid Based Complement Alternat Med. 2017 ;2017:5439645. Epub 2017 Feb 16. PMID: 28298934

Abstract Author(s):

Haddad A El Rabey, Madeha N Al-Seeni, Amal S Bakhashwain

Article Affiliation:

Haddad A El Rabey

Abstract:

This study was conducted to compare the ameliorative effect of Nigella sativa and propolis methanol extract on streptozotocin-induced diabetic male rats and treating diabetic nephropathy. Forty male Albino rats were divided into four groups; the first group was the negative control fed standard diet. The other 30 rats were injected with streptozotocin to induce diabetes by a single intravenous injection and then divided equally into three groups; the second group was the positive diabetic control; the third and the fourth groups were treated orally with 20% w/w Nigella sativa seeds methanol extract and propolis methanol extract (20% w/w), respectively. The rats of the second group showed increased glucose levels and lipid peroxide accompanied with reduction in superoxide dismutase, catalase, and glutathione-S-transferase enzyme activities compared with the negative control. Carboxymethyl lysine, interleukin-6, and immunoglobulins were also increased as a result of diabetes. Kidney function parameters were also elevated, while potassium and sodium levels were decreased. Moreover, tissues of kidney and pancreas showed severe histopathological changes. Treating the diabetic rats with Nigella sativa and propolis methanol extract in the third and fourth groups, respectively, ameliorated all altered biochemical and pathological examinations approaching the negative control. Propolis was more effective than Nigella sativa.

Article Published Date : Dec 31, 2016

Study Type : Animal Study

[87] N. sativa and thymoquinone may prove clinically useful in the treatment of diabetics and in the protection of β-cells against oxidative stress

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Abstract Title:

Effects of Nigella sativa and thymoquinone on biochemical and subcellular changes in pancreaticβ-cells of streptozotocin-induced diabetic rats.

Abstract Source:

J Diabetes. 2010 Dec ;2(4):256-66. PMID: 20923501

Abstract Author(s):

Nabila E Abdelmeguid, Rajaa Fakhoury, Salwa M Kamal, Rana J Al Wafai

Article Affiliation:

Nabila E Abdelmeguid

Abstract:

BACKGROUND: The present study investigated the effects of Nigella sativa aqueous extract and oil, as well as thymoquinone, on serum insulin and glucose concentrations in streptozotocin (STZ) diabetic rats.

METHODS: Rats were divided into five experimental groups (control, untreated STZ-diabetic, and aqueous extract-, oil-, or thymoquinone-treated diabetic rats). Treated rats received 2 mL/kg, i.p., 5%N. sativa extract, 0.2 mL/kg, i.p., N. sativa oil, or 3 mg/mL, i.p., thymoquinone 6 days/week for 30 days. Serum insulin and glucose concentrations, superoxide dismutase (SOD) levels, and pancreatic tissue malondialdehyde (MDA) were determined. Electron microscopy was used to identify any subcellular changes.

RESULTS: Diabetes increased tissue MDA and serum glucose levels and decreased insulin and SOD levels. Treatment of rats with N. sativa extract and oil, as well as thymoquinone, significantly decreased the diabetes-induced increases in tissue MDA and serum glucose and significantly increased serum insulin and tissue SOD. Ultrastructurally, thymoquinone ameliorated most of the toxic effects of STZ, including segregated nucleoli, heterochromatin aggregates (indicating DNA damage), and mitochondrial vacuolization and fragmentation. The aqueous extract of N. sativa also reversed these effects of STZ, but to a lesser extent. The N. sativa oil restored normal insulin levels, but failed to decrease serum glucose concentrations to normal.

CONCLUSIONS: The biochemical and ultrastructural findings suggest that N. sativa extract and thymoquinone have therapeutic and protect against STZ-diabetes by decreasing oxidative stress, thus preserving pancreaticβ-cell integrity. The hypoglycemic effect observed could be due to amelioration of β-cell ultrastructure, thus leading to increased insulin levels. Consequently, N. sativa and thymoquinone may prove clinically useful in the treatment of diabetics and in the protection of β-cells against oxidativestress.

Article Published Date : Nov 30, 2010

Study Type : Animal Study

[88] N. sativa contains potent bioactive components that may aid in treatment against cisplatin induced renal toxicity

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Abstract Title:

Effect of Nigella sativa against cisplatin induced nephrotoxicity in rats.

Abstract Source:

Ital J Food Saf. 2018 Jul 3 ;2(7):7242. Epub 2018 Jul 3. PMID: 30046560

Abstract Author(s):

Amnah M A Alsuhaibani

Article Affiliation:

Amnah M A Alsuhaibani

Abstract:

In this study, the gross composition and mineral content ofseed powder (NSP) and fatty acid composition ofoil (NSO) were investigated. The ability of NSP, extract (NSE) and NSO in reducing the effects of cisplatin-induced renal toxicity in Sprague-Dawley rats were examined. The obtained results showed that NSP contains high amounts of carbohydrates, protein, and fiber while NSO has higher amounts of linoleicacid, oleic acid, and myristic acid. Rats treated with NSP, NSO, and NSE exhibitedreducedserum levels of urea, creatinine, and potassium, and a significant increase of Na, Na/K, vitamin D, nutritional markers, and antioxidant enzymes compared to the cisplatin-induced renal toxicity group receiving noseed treatment. This study determined that all powder, oil, and extracts ofcontain potent bioactive components that may aid in treatment against cisplatininduced renal toxicity in rats.

Article Published Date : Jul 02, 2018

Study Type : Animal Study

[89] N. sativa could be used in the treatment of rhinosinusitis diseases

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Abstract Title:

The value of Nigella sativa in the treatment of experimentally induced rhinosinusitis.

Abstract Source:

Acta Otorhinolaryngol Ital. 2017 Feb ;37(1):32-37. PMID: 28374868

Abstract Author(s):

O Yoruk, A Tatar, O N Keles, A Cakir

Article Affiliation:

O Yoruk

Abstract:

The aim of this study was to investigate the effect of Nigella sativa and cephalexin in the therapy of experimental bacterial rhinosinusitis. Bacterial rhinosinusitis was induced with Staphylococcus aureus. Rabbits were divided into five groups; control (n = 6), N. sativa 50 mg/ kg/d (n = 6), N. sativa 100 mg/kg/d (n = 6), N. sativa 200 mg/kg/d (n = 6), and cephalexin 20 mg/kg/d (n = 6) groups. N. sativa was given orally for 7 days. The same volume of normal saline (0.9% NaCl) was given as a vehicle to the control group for the same period. After treatment period, sinus mucosa samples were evaluated using stereological and histopathological methods. Half of the maxillary sinus mucosa samples were frozen at -800C for further analysis of NO levels. Pathology revealed a severe acute inflammatory process in rabbits treated with saline. Only mild inflammation was determined in cephalexin group, N. sativa 100 mg/kg/d and N. sativa 200 mg/kg/d groups. The level of NO increased in the saline group was significantly reduced in all treatment groups. N. sativa may prevent histopathological changes of rhinosinusitis via decreased NO levels in a dose dependent manner and can be used in the treatment of rhinosinusitis diseases.

Article Published Date : Jan 31, 2017

Study Type : Animal Study

[90] N. sativa exerts a protective effect against cadmium toxicity

Abstract Title:

Effect of black cumin (Nigella sativa) on cadmium-induced oxidative stress in the blood of rats.

Abstract Source:

Biol Trace Elem Res. 2005 Dec ;107(3):277-87. PMID: 16286683

Abstract Author(s):

Mehmet Kanter, Omer Coskun, Ahmet Gurel

Article Affiliation:

Mehmet Kanter

Abstract:

The protective effect of black cumin (Nigella sativa = NS) on cadmium-induced oxidative stress was studied in rats. The rats were randomly divided into three experimental groups: A (conrol), B (Cd treated), and C (Cd + NS treated), each containing 10 animals. The Cd-treated and Cd + NS-treated groups were injected subcutaneously daily with CdCl2 dissolved in isotonic NaCl in the amount of 2 mL/kg for 30 d, resulting in a dosage of 0.49 mg Cd/kg/d. The control group was injected with only isotonic NaCl (2 mL/kg/d) throughout the experiment (for 30 d). Three days prior to induction of CdCl2, the Cd + NS-treated group received a daily intraperitoneal injection of 0.2 mL/kg NS until the end of the study. Cd treatment increased significantly the malondialdehyde levels in plasma and erythrocyte (p<0.01 and p<0.05, respectively) and also increased significantly the antioxidant levels (superoxide dismutase, glutathione peroxidase, and catalase) (p<0.05) compared to the control group. Cd + NS treatment decreased significantly the elevated malondialdehyde levels in plasma and erythrocyte (p<0.01 and p<0.05, respectively) and also reduced significantly the enhanced antioxidant levels (p<0.05). Cd treatment increased significantly the activity of iron levels (p<0.05) in the plasma compared to the control group. Cd + NS treatment decreased the activity of iron levels (p<0.05) in the plasma compared to the Cd-treated group. In the control group with no treatment, histology of erythrocytes was normal. In the Cd-treated group, there were remarkable membrane destruction and hemolytic changes in erythrocytes. In the Cd + NS-treated group, these changes were less than in the Cd-treated group. Our results show that N. sativa exerts a protective effect against cadmium toxicity.

Article Published Date : Nov 30, 2005

Study Type : Animal Study

[91] N. sativa extract and vitamin E improved the serum and urine biochemical parameters and kidney function in cisplatin-induced nephrotoxicity

Abstract Title:

The protective effect of Nigella sativa against cisplatin-induced nephrotoxicity in rats.

Abstract Source:

Avicenna J Phytomed. 2016 Jan-Feb;6(1):44-54. PMID: 27247921

Abstract Author(s):

Sara Hosseinian, Abolfazl Khajavi Rad, Mousa-Al-Reza Hadjzadeh, Nema Mohamadian Roshan, Shahrzad Havakhah, Somayeh Shafiee

Article Affiliation:

Sara Hosseinian

Abstract:

OBJECTIVE: The clinical use of cisplatin is highly restricted, because of its nephrotoxicity. In this study the protective effect of Nigella sativa (N. sativa) against cisplatin-induced nephrotoxicity was investigated in rats.

MATERIALS AND METHODS: In the current study, the effects of the administration of aqueous-ethanolic extract of N. sativa (100 and 200 mg/kg, BW) and vitamin E (100 mg/kg, BW) against blood and urine biochemical alterations and kidney function in rats treated with cisplatin were investigated. Cisplatin was injected at a dose of 6 mg/kg, BW, on the sixth day of the experiment.

RESULTS: The results indicated significant changes in serum urea and creatinine concentration, urine glucose concentration, and urine output in cisplatin group compared with control group. Serum urea and creatinine concentration in preventive and preventive+treatment vitamin E and preventive+treatment N. sativa (200 mg/kg, BW) groups and also serum creatinine concentration in preventive+treatment N. sativa (100 mg/kg, BW) group significantly decreased compared with cisplatin group. Urine glucose concentration in preventive and preventive+treatment N. sativa groups and urine output in preventive and preventive+treatment N. sativa (200 mg/kg, BW) groups significantly decreased compared with cisplatin group.Osmolarity excretion rate in preventive and preventive+treatment vitamin E and preventive N. sativa groups was significantly higher than control group.

CONCLUSIONS: The current study suggests that N. sativa extract and vitamin E in a dose- and time-dependent manner improved the serum and urine biochemical parameters and kidney function in cisplatin-induced nephrotoxicity in rats. However, it needs more investigations to determine the mechanism of N. sativa action on cisplatin-induced kidney toxicity.

Article Published Date : Dec 31, 2015

Study Type : Animal Study

[92] N. sativa extract has potential to prevent the hippocampal neural damage after inducing hypothyroidism during neonatal and juvenile growth in rats

Abstract Title:

Neuroprotective effects of Nigella sativa extract upon the hippocampus in PTU-induced hypothyroidism juvenile rats: A stereological study.

Abstract Source:

Metab Brain Dis. 2017 May 11. Epub 2017 May 11. PMID: 28497360

Abstract Author(s):

Farimah Asiaei, Alireza Fazel, Ali Akbar Rajabzadeh, Mahmoud Hosseini, Farimah Beheshti, Masoumeh Seghatoleslam

Article Affiliation:

Farimah Asiaei

Abstract:

This study aimed to examine the neuroprotective effects of Nigella sativa (N. sativa) in the hippocampus of propylthiouracil (PTU)-induced hypothyroid rats during neonatal and juvenile growth. Twenty- five pregnant rats from early gestation (GD 0) were divided into five groups: (1) control (received drinking water), (2) PTU (received 0.005% PTU in drinking water), (3-5) PTU + NS 0.05%, PTU + NS 0.1%, PTU + NS 0.2% (along with PTU, received 0.05%, 0.1% and 0.2% W/V of N. sativa respectively) and treatment continued until postnatal day 60 (PN 60). The brains of male pups were removed for histological and stereological assessments. N. sativa extract significantly reduced the production of dark neurons and apoptotic cells in different areas of the hippocampus compared to the PTU group. Moreover, it significantly attenuated the effect of hypothyroidism on the volume reduction of the hippocampus. The results of the present study suggested that N. sativa extracthas a potential ability to prevent the hippocampal neural damage after inducing hypothyroidism during neonatal and juvenile growth in rats.

Article Published Date : May 10, 2017

Study Type : Animal Study

[93] N. Sativa extract is effective for early and late prevention of pulmonary fibrosis and inflammation

Article Publish Status: FREE

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Abstract Title:

Early and late preventive effect ofon the bleomycin-induced pulmonary fibrosis in rats: An experimental study.

Abstract Source:

Avicenna J Phytomed. 2018 May-Jun;8(3):263-275. PMID: 29881712

Abstract Author(s):

Hamid Reza Poursalehi, Mitra Samareh Fekri, Fariba Sharifi Far, Ali Mandegari, Atefe Izadi, Rahil Mahmoodi, Hadi Nematollahi, Fateme Porgholamhosein, Vahideh Ghorani, Masome Samareh Fekri

Article Affiliation:

Hamid Reza Poursalehi

Abstract:

Objective: Pulmonary fibhrosis is a disease of the connective tissues in the respiratory system.has been used for the treatment of pulmonary diseases like asthma. This study investigated the early and late preventive effect of methanolic extract ofon a bleomycin- induced pulmonary fibrosis model.

Materials and Methods: This study was carried out using 52 rats. Pulmonary fibrosis was induced by a single endotracheal injection of bleomycin (5 mg/kg). Extract of(500 mg/kg per day) or methylprednisolone succinate (4 mg/kg per day) was injected intraperitoneally in two periods (i.e. days 1-14 as early preventive group and days 15-28 days as late preventive group). The lung tissues were histologically examined at the end of each period and inspected for the amount of hydroxyproline and biomarkers of oxidative stress.

Results: The pulmonary inflammation and fibrosis were significantly decreased in groups treated with methylprednisolone andextract compared to bleomycin group in both early and late prevention groups (p<0.001). The hydroxyproline concentration in pulmonary tissue was significantly decreased inand methylprednisolone groups compared to the bleomycin group in both prevention groups (p<0.001). Significant reductions in lipid peroxidation (p<0.001) and increases in catalase activity were also observed inand methylprednisolone groups compared to bleomycin group.

Conclusion: This study suggested thatextract is effective for early and late prevention of pulmonary fibrosis and inflammation. However, more studies are needed to identify its anti-inflammatory and anti-fibrotic mechanisms in the respiratory system.

Article Published Date : Apr 30, 2018

Study Type : Animal Study

[94] N. sativa may be used to minimize the risk of diabetic microangiopathy

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Abstract Title:

Minimization of the Risk of Diabetic Microangiopathy in Rats by Nigella sativa.

Abstract Source:

Pharmacogn Mag. 2016 May ;12(Suppl 2):S175-80. Epub 2016 May 11. PMID: 27279704

Abstract Author(s):

Juraiporn Somboonwong, Mariem Yusuksawad, Somboon Keelawat, Sirima Thongruay, Ubon Poumsuk

Article Affiliation:

Juraiporn Somboonwong

Abstract:

BACKGROUND: Microangiopathy is a chronic diabetic complication resulting from metabolic derangements, oxidative stress, and increased pro-inflammatory cytokine production. Nigella sativa Linn. is used as an herbal medicine that exerts hypoglycemic, antilipidemic, anti-inflammatory, and antioxidant effects.

OBJECTIVE: To examine the effects of N. sativa extract on cutaneous microvascular changes in diabetic rats.

MATERIALS AND METHODS: Sprague-Dawley rats were randomly assigned into the following four groups: Untreated and N. sativa-treated normal controls and untreated and N. sativa-treated rats with streptozotocin-induced diabetes. A cold-pressed N. sativa extract was then orally administered (1000 mg/kg/day). After 8 weeks of treatment, the glucose, glycosylated hemoglobin (HbA1c), tumor necrosis factor-alpha (TNF-α), insulin levels, and lipid profile were determined in cardiac blood. Dermal capillary wall thickness was measured in tail skin sections stained with periodic acid-Schiff. Endothelial apoptosis was morphologically evaluated by hematoxylin and eosin staining.

RESULTS: Diabetes significantly reduced the circulating insulin and low-density lipoprotein levels and caused elevations in the glucose, HbA1c, and triglyceride levels, accompanied by a slight increase in total cholesterol levels and no change in the high-density lipoprotein and TNF-α levels. Capillary basement membrane thickening and a decreased capillary luminal diameter despite no evidence of endothelial cell apoptosis were also observed. N. sativa treatment of diabetic rats reduced the mean HbA1c concentration by 1.4%, enlarged the capillary lumens, and tended to attenuatedermal capillary basement membrane thickening without affecting the lipid profile or TNF-α level.

CONCLUSION: Our results indicate that N. sativa may be used to minimize the risk of diabetic microangiopathy, potentially due in part to its glycemic control activity.

SUMMARY: Diabetes causes dermal capillary basement membrane thickening and a decreased capillary luminal diameterNigella sativa treatment of diabetic rats enlarged the capillary lumens and tended to attenuate dermal capillary basement membrane thickeningN. sativa treatment of diabetic rats reduced the mean glycosylated hemoglobin concentration by 1.4%, which exceeds the necessary reduction previously described to decrease the risk of diabetic microangiopathy, without affecting the lipid profile or tumor necrosis factor-alpha levelN. sativa improves rat diabetic microangiopathy, potentially due in part to its glycemic control activity. Abbreviations used: H and E: Hematoxylin and eosin, HbA1c: Glycosylated hemoglobin, HDL-C: High-density lipoprotein cholesterol, LDL-C: Low-density lipoprotein cholesterol, PAS: Periodic acid-Schiff, STZ: Streptozotocin.

Article Published Date : Apr 30, 2016

Study Type : Animal Study

[95] N. sativa may be useful in ameliorating signs of gentamicin nephrotoxicity in rats

Abstract Title:

The effect of Nigella sativa oil on gentamicin nephrotoxicity in rats.

Abstract Source:

Am J Chin Med. 2004 ;32(1):49-55. PMID: 15154284

Abstract Author(s):

B H Ali

Article Affiliation:

B H Ali

Abstract:

The pathogenesis of gentamicin (GM) nephrotoxicity has been shown to involve the generation of oxygen free radicals, and several free radical scavengers have been shown to ameliorate the nephrotoxicity. The seeds and oil of Nigella sativa are reported to possess strong antioxidant properties and was effective against disease and chemically-induced hepatotoxicity and nephrotoxicity. Therefore, in the present work, we have tested whether oral treatment of rats with N. sativa oil (0.5, 1.0 or 2.0 ml/kg/day for 10 days) would ameliorate nephrotoxicity of GM (80 mg/kg/day given intramuscularly and concomitantly with the oil during the last 6 days of treatment). Nephrotoxicity was evaluated histopathologically with a light microscope and by measurement of concentrations of urea, creatinine and total antioxidant status (TAS) in plasma and reduced glutathione (GSH) and TAS in kidney cortex. The results indicated that GM treatment caused moderate proximal tubular damage, significantly increased the concentrations of creatinine and urea, and decreased that of TAS and GSH. Treatment with N. sativa oil produced a dose-dependent amelioration of the biochemical and histological indices of GM nephrotoxicity that was statistically significant at the two higher doses used. Compared to controls, treatments of rats with N. sativa did not cause any overt toxicity, and it increased GSH and TAS concentrations in renal cortex and enhanced growth. The results suggest that N. sativa may be useful in ameliorating signs of GM nephrotoxicity in rats, and pending further experimentation to determine safety and efficacy, may be useful clinically.

Article Published Date : Dec 31, 2003

Study Type : Animal Study

[96] N. sativa oil is a useful choice for the treatment of anxiety

Abstract Title:

Repeated administration of Nigella sativa decreases 5-HT turnover and produces anxiolytic effects in rats.

Abstract Source:

Pak J Pharm Sci. 2009 Apr ;22(2):139-44. PMID: 19339222

Abstract Author(s):

Tahira Perveen, Saida Haider, Sumera Kanwal, Darakhshan Jabeen Haleem

Article Affiliation:

Tahira Perveen

Abstract:

The black cumin or Nigella sativa L. seeds have many acclaimed medicinal properties. Pharmacological studies have been conducted on the aqueous and methanol extracts of N. sativa L. seeds to evaluate their effects on the central nervous system. In the present study, N. sativa oil was used to study its effect on anxiety in rats. Open field and elevated plus maze models were selected for the evaluation of anxiolytic effect of drug. After four weeks of daily administration of drug, the rats exhibited an increase in open field activity. The drug also produced anti-anxiety effect in rats when tested in elevated plus maze. Concentrations of 5-HT, 5-HIAA in brain and concentrations of plasma and brain tryptophan determined by HPLC-EC detector. Result shows that oral administration of N. sativa oil increased brain levels of 5-HT but the levels of brain 5-HIAA decreased significantly. Brain and plasma levels of tryptophan also increased significantly following oral repeated administration of N. sitiva oil. Based on this, it may be suggested that N. sativa oil is a useful choice for the treatment of anxiety.

Article Published Date : Mar 31, 2009

Study Type : Animal Study

[97] N. sativa seeds appeared to be safe and possibly protective against carbon tetrachloride induced hepatotoxicity

Abstract Title:

Protective effect of Nigella sativa seeds against carbon tetrachloride-induced liver damage.

Abstract Source:

Am J Chin Med. 2003 ;31(5):721-8. PMID: 14696675

Abstract Author(s):

Mastour S Al-Ghamdi

Article Affiliation:

Mastour S Al-Ghamdi

Abstract:

It has been reported that Nigella sativa oil possesses hepatoprotective effects in some models of liver toxicity. However, it is N. sativa seeds that are used in the treatment of liver ailments in folk medicine rather than its oil. Therefore, the aim of this study was to investigate the effect of the aqueous suspension of N. sativa on carbon tetrachloride (CCL4)-induced liver damage. Aqueous suspension of the seeds was given orally at two dose levels (250 mg/kg and 500 mg/kg) for five days. CCL4 (250 microl/kg intraperitoneally / day in olive oil) was given to the experimental group on days 4 and 5, while the control group was only treated with the vehicles. Animals treated with CCL4 showed remarkable centrilobular fatty changes and moderate inflammatory infiltrate in the form of neutrophil and mononuclear cells when compared to the controls. This effect was significantly decreased in animals pretreated with N. sativa. Histopathological or biochemical changes were not evident following administration of N. sativa alone. Serum levels of aspartic transaminase (AST), and L-alanine aminotransferase (ALT) were slightly decreased while lactate dehydrogenase (LDH) was significantly increased in animals treated with CCL4 when compared to the control group. LDH was restored to normal but ALT and AST levels were increased in animals pretreated with N. sativa. In conclusion, N. sativa seeds appeared to be safe and possibly protective against CCL4-induced hepatotoxicity. However, further studies may still be needed prior to supporting its use in folk medicine for hepatic diseases.

Article Published Date : Dec 31, 2002

Study Type : Animal Study

[98] N. sativa treatment ameliorated the pentylenetetrazol induced neurodegeneration in the cerebral cortex

Abstract Title:

Effects of Nigella sativa on apoptosis and GABAA receptor density in cerebral cortical and hippocampal neurons in pentylenetetrazol induced kindling in rats.

Abstract Source:

Biotech Histochem. 2016 Nov ;91(8):493-500. PMID: 27849392

Abstract Author(s):

I Meral, M Esrefoglu, K A Dar, S Ustunova, M S Aydin, M Demirtas, Y Arifoglu

Article Affiliation:

I Meral

Abstract:

We investigated the effects of Nigella sativa on apoptosis and gamma-aminobutyric acid (GABAA) receptor density in cerebral cortical and hippocampal neurons in a pentylenetetrazol (PTZ)-induced kindling model in rats. The PTZ kindling model was produced by injecting PTZ in subconvulsive doses to rats on days 1, 3, 5, 8, 10, 12, 15, 17, 19, 22 and 24 of the study into animals of PTZ treated (PTZ) and PTZ + N. sativa treated (PTZ + NS) groups. Clonic and tonic seizures were induced by injecting a convulsive dose of PTZ on day 26 of the study. Rats in the PTZ + NS group were treated also with a 10 mg/kg methanolic extract of N. sativa 2 h before each PTZ injection. Rats in the control group were treated with 4 ml/kg saline. The number of neurons that expressed GABAA receptors in the hippocampus and cerebral cortex of rats in the PTZ and PTZ + NS groups increased significantly. There was no significant difference in the number of GABAA receptors between the PTZ and PTZ + NS groups. GABAA receptor density of the neurons in the cerebral cortex, but not hippocampus, was increased in PTZ group compared to controls. We observed a significant increase in the number of apoptotic neurons in the cerebral cortex of rats of both the PTZ and PTZ + NS groups compared to controls. We observed a significant decrease in the number of the apoptotic neurons in the cerebral cortex of rats in the PTZ + NS group compared to the PTZ group. N. sativa treatment ameliorated the PTZ induced neurodegeneration in the cerebral cortex as reflected by neuronal apoptosis and neuronal GABAA receptor frequency.

Article Published Date : Oct 31, 2016

Study Type : Animal Study

[99] Nigella saliva seeds can protect against lead acetate induced hepatic oxidative damage

Abstract Title:

Hepatoprotective efficacy of Nigella sativa seeds dietary supplementation against lead acetate-induced oxidative damage in rabbit - Purification and characterization of glutathione peroxidase.

Abstract Source:

Biomed Pharmacother. 2017 Mar 3 ;89:711-718. Epub 2017 Mar 3. PMID: 28273633

Abstract Author(s):

Ali H El-Far, Mahdy A Korshom, Abdelwahab A Mandour, Ashraf A El-Bessoumy, Yasser S El-Sayed

Article Affiliation:

Ali H El-Far

Abstract:

Lead (Pb) is a toxic ubiquitous environmental pollutant that induces hepatotoxicity in both animals and humans. The ability of Nigella saliva seeds (NSS) in ameliorating lead acetate (PbAc)-induced hepatic oxidative damage was investigated using a rabbit model. Forty New Zealand rabbits were given feed and water ad libitum. They were allocated randomly into four groups: control; PbAc (5g/L drinking water); NSS (20g/kg diet) and NSS+PbAc groups. After two months, liver samples were collected and analyzed for malondialdehyde (MDA), glutathione (GSH), glutathione S-transferase (GST) and glutathione peroxidase (GPx) contents. Purification and characterization of GPx were also evaluated. PbAc exposure significantly (p<0.05) increased MDA (lipid peroxidation biomarker) and reduced the GSH levels and the GST and GPx activities. Concurrently supplemented NSS significantly (p<0.05) decreased MDA levels and restored the GSH, GST, and GPx contents successfully. Electrophoretically, the homogeneous GPx preparation from the liver had a specific activity of 30.44 U/mg protein and a yield of 1.31%. The Km values for cumene hydroperoxide were 4.76μM in control, PbAc and NSS+PbAc groups, and 4.09μM in NSS group. The GPx reaction had a temperature optimum 40°C, pH optimum 8 and molecular weight 21 kDa. The obtained data indicated the potent efficacy of NSS against PbAc-induced oxidative stress; that was mediated through induction and activation of antioxidants, particularly GPx and scavenging free radicals. Moreover, the purified hepatic GPx is characterized as a selenoprotein (Se-GPx).

Article Published Date : Mar 02, 2017

Study Type : Animal Study

[100] Nigella sativa (black cumin) and Syzygium aromaticum (clove) protect against aflatoxicosis in rats

Abstract Title:

Antioxidant property of Nigella sativa (black cumin) and Syzygium aromaticum (clove) in rats during aflatoxicosis.

Abstract Source:

J Appl Toxicol. 2005 May-Jun;25(3):218-23. PMID: 15856529

Abstract Author(s):

M A Abdel-Wahhab, S E Aly

Abstract:

Aflatoxins, a group of closely related, extremely toxic mycotoxins produced by Aspergillus flavus and A. parasiticus, can occur as natural contaminants of foods and feeds. Aflatoxins have been shown to be hepatotoxic, carcinogenic, mutagenic and teratogenic to different animal species. Nigella sativa (black cumin) and Syzygium aromaticum (clove) oil are used for the treatment of inflammatory diseases and have antioxidant properties. The aim of this study was to investigate the ability of these volatile oils to scavenge free radicals generated during aflatoxicosis. Sixty male rats were divided into six treatment groups, including a control group, and the groups were treated for 30 days with Nigella sativa and Syzygium aromaticum oils with or without aflatoxin. Blood samples were collected at the end of the experimental period for haematological and biochemical analysis. The results indicated that exposure to aflatoxins resulted in haematological and biochemical changes typical for aflatoxicosis. Treatment with Nigella sativa and Syzygium aromaticum oil of rats fed an aflatoxin-contaminated diet resulted in significant protection against aflatoxicosis. Moreover, Nigella sativa oil was found to be more effective than Syzygium aromaticum oil in restoring the parameters that were altered by aflatoxin in rats.

Article Published Date : May 01, 2005

Study Type : Animal Study

[101] Nigella sativa (black cumin) protects against chronic antiretroviral therapy-associated insulin resistance in HIV-1 infected rats

Abstract Title:

Therapeutic effects of Nigella sativa on chronic HAART-induced hyperinsulinemia in rats.

Abstract Source:

Can J Physiol Pharmacol. 2009 Apr;87(4):300-9. PMID: 19370083

Abstract Author(s):

Surabhi Chandra, Subramanyam N Murthy, Debasis Mondal, Krishna C Agrawal

Article Affiliation:

Department of Pharmacology, Tulane University Health Sciences Center, 1430 Tulane Avenue, SL-83, New Orleans, LA 70112, USA.

Abstract:

Prolonged use of highly active antiretroviral therapy (HAART) is associated with insulin resistance in HIV-1-positive patients. Small animal models that recapitulate the long-term effects of HAART may facilitate the identification of therapeutic agents to suppress these side effects. We investigated the protective effects of black seed oil (BSO) from Nigella sativa in Sprague-Dawley rats treated with a daily HAART regimen for 7 months. The antiretroviral drugs, consisting of nelfinavir (200 mg/kg), zidovudine (50 mg/kg), and efavirenz (20 mg/kg), were mixed with diet with or without BSO (400 microL/kg) supplementation. Significant increases in insulin and C-peptide levels were observed in HAART-treated groups, and concomitant BSO treatment reduced this hyperinsulinemia. Interestingly, HAART-treated rats showed reduced size of pancreatic islets that was not seen in BSO-exposed rats. In vitro studies showed that nelfinavir, alone and in combination with HAART, induced oxidative stress and decreased glucose-induced insulin production in INS-1 cells. Suppressed insulin production was restored in cells coexposed to either BSO or thymoquinone. Our findings demonstrated that chronic HAART may increase serum insulin levels by dysregulating both insulin production by beta cells and insulin action at the periphery. These deleterious effects may be prevented by dietary supplementation with BSO.

Article Published Date : Apr 01, 2009

Study Type : Animal Study

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[102] Nigella sativa acts in the kidney as a potent scavenger of free radicals to prevent the toxic effects of gentamicin

Abstract Title:

Protective effects of nigella sativa against gentamicin-induced nephrotoxicity in rats.

Abstract Source:

Exp Toxicol Pathol. 2010 Mar ;62(2):183-90. Epub 2009 Apr 23. PMID: 19398313

Abstract Author(s):

Ihsan Yaman, Engin Balikci

Article Affiliation:

Ihsan Yaman

Abstract:

The aim of this study was focused on investigating the possible protective effect of NS against GS-induced nephrotoxicity. Twenty four Wistar-albino rats were divided into four equal groups as follows: control group, GS group (100mg/kg intraperitoneal - i.p.), NSL+GS group (0.2 ml/kg+100mg/kg i.p.) and NSH+GS group (0.4 ml/kg+100mg/kg i.p.). Plasma creatinine and urea levels significantly increased as a result of nephrotoxicity in the GS group. Also, creatinine and urea levels significantly decreased in NSL+GS and NSH+GS groups. In the GS group, plasma MDA and NO levels increased significantly (p<0.05) and erythrocyte SOD and GSH-Px activities decreased significantly (p<0.05) when compared with control group. NS administration with GS injection resulted in significantly decreased MDA and NO generation and increased SOD and GSH-Px activities when compared with GS group. Proximal tubular necrosis, vacuolation, desquamation and degeneration in epithelial cells of the proximal tubules, hyaline casts in tubular lumen, mononuclear cell infiltration, glomerular and basement membrane alterations were histopathologically detected in the kidneys of the GS group. Co-treatments with NS (low and high dose) considerably decreased the renal damage when compared with the GS group. In conclusion, NS acts in the kidney as a potent scavenger of free radicals to prevent the toxic effects of GS both in the biochemical and histopathological parameters.

Article Published Date : Feb 28, 2010

Study Type : Animal Study

[103] Nigella sativa and nettle decrease lipid peroxidation and liver enzymes, and increase the antioxidant defence system activity in CCl4-treated rats

Abstract Title:

Effects of Nigella sativa L. and Urtica dioica L. on lipid peroxidation, antioxidant enzyme systems and some liver enzymes in CCl4-treated rats.

Abstract Source:

J Vet Med A Physiol Pathol Clin Med. 2003 Jun ;50(5):264-8. PMID: 14567515

Abstract Author(s):

M Kanter, I Meral, S Dede, H Gunduz, M Cemek, H Ozbek, I Uygan

Article Affiliation:

M Kanter

Abstract:

This study was designed to investigate the effects Nigella sativa L. (NS) and Urtica dioica L. (UD) on lipid peroxidation, antioxidant enzyme systems and some liver enzymes in carbon tetrachloride (CCl4)-treated rats. A total of 60 healthy male Sprague-Dawley rats were utilized in this study. The rats were randomly allotted into one of four experimental groups: A (CCl4-only treated), B (CCl4 + UD treated), C (CCl4 + NS treated) and D (CCl4 + UD + NS treated), each containing 15 animals. All groups received CCl4 [0.8 ml/kg of body weight, subcutaneously, twice a week for 90 days starting day 1]. In addition, B, C and D groups also received daily intraperitoneal injections of 0.2 ml/kg NS or/and 2 ml/kg UD oils for 45 days starting day 46. Group A, on the other hand, received only 2 ml/kg normal saline solution for 45 days starting day 46. Blood samples for the biochemical analysis were taken by cardiac puncture from five randomly chosen rats in each treatment group at beginning, at 45th and at 90th day of the experiment. The CCl4 treatment for 45 days increased the lipid peroxidation and liver enzymes, and also decreased the antioxidant enzyme levels. NS or UD treatments (alone or combination) for 45 days starting day 46 decreased the elevated lipid peroxidation and liver enzyme levels and also increased the reduced antioxidant enzyme levels. Live weights of the rats decreased in group A, and increased in groups B, C and D. It is concluded that NS and UD decrease the lipid peroxidation and liver enzymes, and increase the antioxidant defence system activity in the CCl4-treated rats.

Article Published Date : May 31, 2003

Study Type : Animal Study

[104] Nigella sativa and thymoquinone could be a potential treatment against peripheral neuropathy

Abstract Title:

Effects of Nigella sativa and its major constituent, thymoquinone on sciatic nerves in experimental diabetic neuropathy.

Abstract Source:

Neurochem Res. 2008 Jan ;33(1):87-96. Epub 2007 Aug 23. PMID: 17713854

Abstract Author(s):

Mehmet Kanter

Article Affiliation:

Mehmet Kanter

Abstract:

The aim of this study was designed to investigate the possible beneficial effects of Nigella sativa (NS) and thymoquinone (TQ) on histopathological changes of sciatic nerves in streptozotocin-induced diabetic rats. The rats were randomly allotted into one of four experimental groups: A (control), B (diabetic untreated), C (diabetic treated with NS) and D (diabetic treated with TQ); each group contain ten animals. B, C and D groups received streptozotocin (STZ) to induce diabetes. The rats in NS and TQ treated groups were given NS (in a dose of 400 mg/kg body weight) and TQ (50 mg/kg body weight) once a day orally by using intra-gastric intubation for 12 weeks starting 2 days after STZ injection, respectively. Blood and tissue samples were obtained for biochemical and histopathological investigation. The treatment of both NS and TQ caused a sharp decrease in the elevated serum glucose (P<0.01, 0.05, respectively), and an increase in the lowered serum insulin concentrations (P<0.01, 0.05, respectively), in STZ-induced diabetic rats. STZ induced a significant decrease in the area of insulin immunoreactive beta-cells (P<0.0001). NS (P<0.001) and TQ (P<0.01) treatment resulted in increased area of insulin immunoreactive beta-cells significantly. To date, no histopathological changes of sciatic nerves in STZ induced diabetic rats by NS and TQ treatment have been reported. In this study, histologic evaluation of the tissues in diabetic animals treated with TQ and especially NS showed fewer morphologic alterations. Myelin breakdown decreased significantly after treatment with NS and TQ. The ultrastructural features of axons also showed remarkable improvement. We believe that further preclinical research into the utility of NS and TQ may indicate its usefulness as a potential treatment on peripheral neuropathy (PN) in STZ induced diabetic rats.

Article Published Date : Dec 31, 2007

Study Type : Animal Study

[105] Nigella sativa could reduce the chances of hypothyroidism induced gastritis

Article Publish Status: FREE

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Abstract Title:

Gastroprotective effects of Nigella Sativa oil on the formation of stress gastritis in hypothyroidal rats.

Abstract Source:

Int J Physiol Pathophysiol Pharmacol. 2009 Mar 10 ;1(2):143-149. Epub 2009 Mar 10. PMID: 21383883

Abstract Author(s):

Khaled A Abdel-Sater

Article Affiliation:

Khaled A Abdel-Sater

Abstract:

The aim of this study was to assess the effects of hypothyroidism on the development of acute cold restraint stress gastritis in rats and protective effect of Nigella sativa at the beginning of the acute cold restraint stress. 60 rats were randomly divided into six groups; the control (groups I), surgically thyroidectomized group (group II), acute cold restraint stressed group (group III), surgically thyroidectomized plus stressed group (group IV), Nigella sativa oil group (group V) and surgically thyroidectomized plus stressed plus Nigella sativa oil group (group VI). Volume of gastric juice, number and size of ulcer, gastric malonaldehyde, gastric glutathione, gastric protein and serum thyroxine (T4) were measured. Significant increases both of number of gastric ulcer and malonaldehyde while decreases both of glutathione and protein levels in rats in groups III and IV in comparison with control group. While, insignificant increase were observed between control and both of groups II and VI. In rats, low thyroid hormone level increase stress gastritis, and this effect can be decreased by treatment with Nigella sativa.

Article Published Date : Mar 09, 2009

Study Type : Animal Study

[106] Nigella sativa exerts a therapeutic effect on cholestatic liver injury in bile duct ligated rats

Abstract Title:

The effects of Nigella sativa on bile duct ligation induced-liver injury in rats.

Abstract Source:

Cell Biochem Funct. 2010 Jan ;28(1):83-8. PMID: 20029957

Abstract Author(s):

Sacid Coban, Fahrettin Yildiz, Alpaslan Terzi, Behcet Al, Nurten Aksoy, Muharrem Bitiren, Hakim Celik

Article Affiliation:

Sacid Coban

Abstract:

Nigella sativa (NS) has been shown to have antioxidant and antiinflammatory activities in different conditions. The goal of this study was to evaluate the effects of NS on cholestatic liver injury in rats. Thirty rats were recruited in the study as follows: Group 1, Bile duct ligation (BDL) (n = 10); Group 2, BDL plus NS (n = 10); and Group 3, Sham (n = 10). Bile duct ligated group received 0.2 mL kg(-1) dose of NS intraperitoneally daily throughout 14 days. Liver damage and cholestasis were determined by the biochemical and the pathologic examination. Data showed a decrease in gamma glutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) activities of the NS treated rats when compared with BDL group (p<0.001 for GGT and p<0.05 for others). The NS treated rats' tissue levels of total oxidant status (TOS), oxidative stress index (OSI), and myeloperoxidase (MPO) were significantly lower than that of the BDL group (p<0.01 for all). Increases in total antioxidant capacity (TAC) and catalase (CAT) levels were statistically significant in the NS treated rats compared to BDL group (p<0.01 for both). On the other hand, administration of NS in the rats with biliary obstruction resulted in inhibition of necro-inflammation. These results indicate that NS exerts a therapeutic effect on cholestatic liver injury in bile duct ligated rats possibly through attenuation of enhanced neutrophil infiltration and oxidative stress in the liver tissue.

Article Published Date : Dec 31, 2009

Study Type : Animal Study

[107] Nigella sativa exerts a therapeutic protective effect in diabetes by decreasing oxidative stress and preserving pancreatic beta-cell integrity

Article Publish Status: FREE

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Abstract Title:

Effects of Nigella sativa on oxidative stress and beta-cell damage in streptozotocin-induced diabetic rats.

Abstract Source:

Anat Rec A Discov Mol Cell Evol Biol. 2004 Jul ;279(1):685-91. PMID: 15224410

Abstract Author(s):

Mehmet Kanter, Omer Coskun, Ahmet Korkmaz, Sukru Oter

Article Affiliation:

Mehmet Kanter

Abstract:

The aim of the present study was to evaluate the possible protective effects of Nigella sativa L. (NS) against beta-cell damage from streptozotocin (STZ)-induced diabetes in rats. STZ was injected intraperitoneally at a single dose of 50 mg/kg to induce diabetes. NS (0.2 ml/kg/day, i.p.) was injected for 3 days prior to STZ administration, and these injections were continued throughout the 4-week study. Oxidative stress is believed to play a role in the pathogenesis of diabetes mellitus (DM). To assess changes in the cellular antioxidant defense system, we measured the activities of antioxidant enzymes (such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD), and catalase (CAT)) in pancreatic homogenates. We also measured serum nitric oxide (NO) and erythrocyte and pancreatic tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, to determine whether there is an imbalance between oxidant and antioxidant status. Pancreatic beta-cells were examined by immunohistochemical methods. STZ induced a significant increase in lipid peroxidation and serum NO concentrations, and decreased antioxidant enzyme activity. NS treatment has been shown to provide a protective effect by decreasing lipid peroxidation and serum NO, and increasing antioxidant enzyme activity. Islet cell degeneration and weak insulin immunohistochemical staining was observed in rats with STZ-induced diabetes. Increased intensity of staining for insulin, and preservation of beta-cell numbers were apparent in the NS-treated diabetic rats. These findings suggest that NS treatment exerts a therapeutic protective effect in diabetes by decreasing oxidative stress and preserving pancreatic beta-cell integrity. Consequently, NS may be clinically useful for protecting beta-cells against oxidative stress.

Article Published Date : Jun 30, 2004

Study Type : Animal Study

[108] Nigella sativa exerts lipid-lowering and insulin-sensitizing actions in the rat

Abstract Title:

The petroleum ether extract of Nigella sativa exerts lipid-lowering and insulin-sensitizing actions in the rat.

Abstract Source:

J Ethnopharmacol. 2004 Oct ;94(2-3):251-9. PMID: 15325727

Abstract Author(s):

Phuong Mai Le, Ali Benhaddou-Andaloussi, Aziz Elimadi, Abdellatif Settaf, Yahia Cherrah, Pierre S Haddad

Article Affiliation:

Phuong Mai Le

Abstract:

We studied the effect of a 4-week intragastric gavage with a petroleum ether extract of Nigella sativa seeds on blood glucose, insulin and lipids in the normal rat. Petroleum ether extract caused a 25% reduction in food intake that translated into a transient weight loss. No sign of toxicity of the plant could be seen in vivo or in vitro. Fasting plasma glucose remained stable throughout Nigella sativa treatment. At the end of the 4-week treatment, Nigella sativa-treated rats had lower fasting plasma levels of insulin and triglycerides, and higher HDL-cholesterol as compared to pair-fed controls. Response to insulin was evaluated in hepatocytes isolated from animals of all groups by Western blot analysis of phosphorylated MAPK p44/42erk and PKB. In vivo Nigella sativa treatment resulted in greater dose-dependent activation of MAPK and PKB in response to insulin. These results suggest that the petroleum ether extract of Nigella sativa has a slight anorexic effect, and that it contains the hypolipidemic activity previously obtained with the plant. More significantly, our data demonstrate that in vivo treatment with the petroleum ether extract exerts an insulin-sensitizing action by enhancing the activity of the two major intracellular signal transduction pathways of the hormone's receptor.

Article Published Date : Sep 30, 2004

Study Type : Animal Study

[109] Nigella sativa extract affects conditioned place preference induced by morphine in rats, indicating it may have therapeutic value in treating morphine dependence and/or tolerance

Article Publish Status: FREE

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Abstract Title:

Nigella sativa extract affects conditioned place preference induced by morphine in rats.

Abstract Source:

Anc Sci Life. 2012 Oct;32(2):82-8. doi: 10.4103/0257-7941.118537.

Abstract Author(s):

Milad Anvari, Atefeh Seddigh, Mohammad Naser Shafei, Hassan Rakhshandeh, Amir Hossein Talebi, Mohammad Reza Tahani, S Mohsen Saeedjalali, Mahmoud Hosseini

Article Affiliation:

Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract:

OBJECTIVE: Neuroprotective, antioxidant, anticonvulsant, and analgesic effects of Nigella sativa (NS) have been previously shown. The interaction of NS with opioid system has also been reported. In the present study, the effects of NS hydro-alcoholic extract on the acquisition and expression of morphine-induced conditioned place preference (CPP) in rats were evaluated.

MATERIALS AND METHODS: CPP was induced by injection of morphine (5 mg/kg, i.p.) on three consecutive days in compartment A of the CPP apparatus. Injection of NS extract (200 and 400 mg/kg, i.p.) 60 min before morphine administration on the conditioning days and 60 min before the post-conditioning phase was done for the evaluation of acquisition and expression effects, respectively. Conditioning effect of NS extract was also evaluated by injection of extract (200 or 400 mg/kg, i.p.) in the conditioning phase, instead of morphine in different groups. The difference in time which the animals spent in compartment A on the day before conditioning and the days after conditioning was determined and compared between groups.

RESULTS: The time spent by the rats in compartment A in the morphine group was greater than that in the saline group (P<0.01). Both doses of NS extract decreased acquisition of morphine-induced CPP (P<0.01 and P<0.001), but had no significant effect on the expression of morphine CPP. Higher dose of the extract (400 mg) showed a significant conditioning effect which was comparable to the effect of morphine.

CONCLUSION: The results of the present study showed that the hydro-alcoholic extract of NS has conditioning effect. It also decreased acquisition, but had no significant effect on the expression of morphine CPP.

Article Published Date : Sep 30, 2012

Study Type : Animal Study

[110] Nigella sativa extract has an anti-fatigue activity against exhaustive swimming

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Abstract Title:

Nigella sativa seed extract attenuates the fatigue induced by exhaustive swimming in rats.

Abstract Source:

Biomed Rep. 2017 Apr ;6(4):468-474. Epub 2017 Feb 24. PMID: 28413647

Abstract Author(s):

Mahbubur Rahman, Dong Kwon Yang, Gi-Beum Kim, Sei-Jin Lee, Shang-Jin Kim

Article Affiliation:

Mahbubur Rahman

Abstract:

In previous studies, Nigella sativa (NS) has been studied due to its various physiological and pharmacological activities. However, evidence on the effects of NS on physical fatigue following exhaustive swimming remains limited. In the present study, the authors evaluated the potential beneficial effects of NS against the fatigue activity following exhaustive swimming. Rats were orally administered with NS extract (2 g/kg/day) for 21 days, and the anti-fatigue effect was assessed by exhaustive swimming exercise. The presented results indicated that pre-treatment of NS extract significantly increased the time to exhaustion. In hemodynamic parameters, NS extract increased blood pO2 and O2sat, but decreased pCO2. For underlying mechanisms, NS extract protected depletion of energy, indicated by increased levels of blood pH, glucose and tissue glycogen contents, and decreased levels of blood lactate, tissue lactic dehydrogenase and creatine kinase, when the NS extract was pre-treated. In addition, the NS extract inhibited oxidative stress following exhaustive swimming, as reflected by the results of increased levels of superoxide dismutase and redox ratio, and decreased the level of malondialdehyde when the NS extract was pre-treated. Collectively, the present study demonstrated that NS extract has an anti-fatigue activity against exhaustive swimming by energy restoration and oxidative-stress defense.

Article Published Date : Mar 31, 2017

Study Type : Animal Study

[111] Nigella sativa extract possesses hepatoprotective action against paracetamol induced acute hepatoxicity

Abstract Title:

Protective effects of ethanolic extract of Nigella sativa seed in paracetamol induced acute hepatotoxicity in vivo.

Abstract Source:

Pak J Biol Sci. 2014 Apr ;17(4):517-22. PMID: 25911839

Abstract Author(s):

D S Kushwah, M T Salman, P Singh, V K Verma, A Ahmad

Article Affiliation:

D S Kushwah

Abstract:

Paracetamol overdose causes serious liver necrosis. Hepatoprotective activity of ethanolic extract of Nigella sativa in Paracetamol induced acute hepatotoxicity was investigated in rats. Fasted male Wistar rats were orally treated with Nigella sativa extract in graded doses for 5 days followed by Nigella sativa extract and paracetamol 3 g kg(-1) on 6 and 7th day. Circulatory liver markers and reduced glutathione (GSH) levels were estimated and histopathological study of liver performed. Paracetamol caused a significant increase in serum alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and total Bilirubin and a significant decrease in GSH compared to control. Nigella sativa pretreatment significantly prevented the increase in liver enzymes and total bilirubin and decrease in GSH level as compared to paracetamol group. Liver histopathology showed marked reduction in sinusoidal dilatation, midzonal necrosis, portal triaditis and occasional apoptosis in Nigella sativa extract treated groups as compared to group receiving only paracetamol. Nigella sativa extract possesses hepatoprotective action against paracetamol induced acute hepatoxicity. Further research is needed to advocate its prophylactic use for drug induced hepatotoxicity.

Article Published Date : Mar 31, 2014

Study Type : Animal Study

[112] Nigella sativa has a protective roll against the initiation of colon carcinogenesis

Abstract Title:

Role of Nigella sativa and a number of its antioxidant constituents towards azoxymethane-induced genotoxic effects and colon cancer in rats.

Abstract Source:

Phytother Res. 2008 Oct ;22(10):1311-23. PMID: 18570215

Abstract Author(s):

Dalal Al-Johar, Neptune Shinwari, Jamal Arif, Nasser Al-Sanea, Alaa Abdul Jabbar, Ra'afat El-Sayed, Abdullah Mashhour, Grisellhi Billedo, Inaam El-Doush, Iman Al-Saleh

Article Affiliation:

Dalal Al-Johar

Abstract:

This study examined the chemopreventive effect of Nigella sativa and some of its antioxidant constituents on a number of colon cancer biomarkers in rats induced with azoxymethane (AOM). Sixty male Sprague-Dawley rats were randomly assigned into ten subgroups: vehicle (1-5) and experimental (6-10). The rats in each group were fed one of the following diets: basal diet, (200 mg/kg) Nigella sativa, (0.2 mg/kg) selenium, (1.2 mg/kg) all-trans-retinol plus (100 mg/kg) DL-alpha-tocopherol and (10 mg/kg) thymoquinone, respectively. Only rats in subgroups 6-10 were injected with AOM (15 mg/kg) once per week for 2 weeks. Both groups were fed their respective diets for 5 weeks. Then they were killed and examined for colonic aberrant crypt foci (ACF). Our result showed that only vitamin supplementation was effective on ACF. Nigella sativa revealed inhibitory effects only on DNA damage (day 34) in the AOM-treated rat group. Alternatively, selenium, thymoquinone and vitamins inhibited the MDA content in the liver. Although the exact mechanisms involved in the protective role of Nigella sativa against the initiation of colon carcinogenesis are not clearly understood, the results suggest that its inhibitory effects might depend on the combined competitive inhibition of various antioxidant constituents of this plant.

Article Published Date : Sep 30, 2008

Study Type : Animal Study

[113] Nigella sativa has a significant nephroprotective activity on paracetamol-induced nephrotoxicity

Abstract Title:

Paracetamol-induced nephrotoxicity and oxidative stress in rats: the protective role of Nigella sativa.

Abstract Source:

Pharm Biol. 2016 Mar 9:1-10. Epub 2016 Mar 9. PMID: 26956915

Abstract Author(s):

Dogukan Canayakin, Yasin Bayir, Nurcan Kilic Baygutalp, Esen Sezen Karaoglan, Hasan Tarik Atmaca, Fatma Betul Kocak Ozgeris, Mevlut Sait Keles, Zekai Halici

Article Affiliation:

Dogukan Canayakin

Abstract:

Context Nigella sativa L. (Ranunculaceae) (NS) is traditionally used to treat many conditions such as inflammation. Objective This study evaluates the effects of NS seeds ethanol extract in paracetamol-induced acute nephrotoxicity in rats. Materials and methods Forty-eight female Wistar Albino rats were divided into eight groups: I = sham; II = sham + 1000 mg/kg NS; III = sham + 140 mg/kg (N-acetyl cysteine) NAC; IV = 2 g/kg paracetamol; V = 2 g/kg paracetamol + 140 mg/kg NAC; VI, VII and VIII = 2 g/kg paracetamol + 250, 500 and 1000 mg/kg NS, respectively. Paracetamol administration (oral) was carried out 1 h after NS and NAC administrations (oral), and all animals were sacrificed 24 h later. Results Paracetamol administration significantly increased serum urea (88.05 U/L) and creatinine (0.80 U/L) when compared with the sham group (49.80 and 0.31 U/L, respectively). However, serum urea level was reduced to 65.60, 56.00 and 54.18 U/L, with 250, 500 and 1000 mg/kg doses of the extract, respectively. Also, serum creatinine level was reduced to 0.64, 0.57 and 0.52 U/L with 250, 500 and 1000 mg/kg doses of the extract, respectively. NS administration increased superoxide dismutase and glutathione, and decreased malondialdehyde levels in the kidneys. Kidney histopathological examinations showed that NS administration antagonized paracetamol-induced kidney pathological damage. Discussion and conclusions The results suggest NS has a significant nephroprotective activity on paracetamol-induced nephrotoxicity. It may be suggested that the antiinflammatory and antioxidant effects of NS ethanolic extract originated from different compounds of its black seeds.

Article Published Date : Mar 08, 2016

Study Type : Animal Study

[114] Nigella sativa has protective effects against radiation-induced damage

Abstract Title:

Protective effects of Nigella sativa on gamma radiation-induced jejunal mucosal damage in rats.

Abstract Source:

Pathol Res Pract. 2016 Feb 17. Epub 2016 Feb 17. PMID: 26944830

Abstract Author(s):

Zeynep Nur Orhon, Cem Uzal, Mehmet Kanter, Mustafa Erboga, Murat Demiroglu

Article Affiliation:

Zeynep Nur Orhon

Abstract:

INTRODUCTION: The aim of this study was to compare the efficacy of Nigella sativa in protection of jejunal mucosa against harmful effects of gamma radiation.

METHODS: Radiotherapy group received abdominal gamma radiation of 15Gy in addition to physiological saline. Radiotherapy+Nigella sativa treatment group received abdominal gamma radiation of 15Gy in addition to Nigella sativa treatment in the amount of 400mg/kg. Radiotherapy and treatment groups were sacrificed 3 days after the exposure to irradiation. Then, jejunum samples were harvested for biochemical and histological assessment of mucosal injury.

RESULTS: Nigella sativa treatment was found to significantly lower elevated tissue malondialdehyde (MDA) levels and, to raise reduced glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activity in intestinal tissues samples. Single dose 15Gy gamma-irradiation was noted to result in a marked jejunal mucosal injury. Three days after exposure to irradiation, the villi and Lieberkühn crypts were observed as denuded, and villous height diminished. Concomitantly with inflammatory cell invasion, capillary congestion and ulceration were observed in the atrophic mucosa. Nigella sativa treatment significantly attenuated the radiation induced morphological changes in the irradiated rat jejunal mucosa.

CONCLUSION: Nigella sativa has protective effects against radiation-induced damage, suggesting that clinical transfer is feasible.

Article Published Date : Feb 16, 2016

Study Type : Animal Study

[115] Nigella sativa is a potent chemopreventive agent and may suppress potassium bromate-mediated renal oxidative stress, toxicity and tumour promotion

Abstract Title:

Nigella sativa (black cumin) ameliorates potassium bromate-induced early events of carcinogenesis: diminution of oxidative stress.

Abstract Source:

Hum Exp Toxicol. 2003 Apr ;22(4):193-203. PMID: 12755470

Abstract Author(s):

Naghma Khan, Sonia Sharma, Sarwat Sultana

Article Affiliation:

Naghma Khan

Abstract:

Potassium bromate (KBrO3) is a potent nephrotoxic agent. In this paper, we report the chemopreventive effect of Nigella sativa (black cumin) on KBrO3-mediated renal oxidative stress, toxicity and tumor promotion response in rats. KBrO3 (125 mg/kg body weight, intraperitoneally) enhances lipid peroxidation, gamma-glutamyl transpeptidase, hydrogen peroxide and xanthine oxidase with reduction in the activities of renal antioxidant enzymes and renal glutathione content. A marked increase in blood urea nitrogen and serum creatinine has also been observed. KBrO3 treatment also enhances ornithine decarboxylase (ODC) activity and [3H] thymidine incorporation into renal DNA. Prophylaxis of rats orally with Nigella sativa extract (50 mg/kg body weight and 100 mg/kg body weight) resulted in a significant decrease in renal microsomal lipid peroxidation (P<0.001), gamma-glutamyl transpeptidase (P<0.001), H2O2 (P<0.001) and xanthine oxidase (P<0.05). There was significant recovery of renal glutathione content (P<0.01) and antioxidant enzymes (P<0.001). There was also reversal in the enhancement of blood urea nitrogen, serum creatinine, renal ODC activity and DNA synthesis (P<0.001). Data suggest that Nigella sativa is a potent chemopreventive agent and may suppress KBrO3-mediated renal oxidative stress, toxicity and tumour promotion response in rats.

Article Published Date : Mar 31, 2003

Study Type : Animal Study

[116] Nigella sativa is a potent chemopreventive agent and suppresses Fe-NTA-induced oxidative stress, hyperproliferative response and renal carcinogenesis

Abstract Title:

Inhibition of two stage renal carcinogenesis, oxidative damage and hyperproliferative response by Nigella sativa.

Abstract Source:

Eur J Cancer Prev. 2005 Apr ;14(2):159-68. PMID: 15785320

Abstract Author(s):

N Khan, S Sultana

Article Affiliation:

N Khan

Abstract:

In an earlier communication we reported that Nigella sativa suppresses potassium bromate-induced renal oxidative damage. In the present study, we report the chemopreventive effect of Nigella sativa against ferric nitrilotriacetate (Fe-NTA)-induced renal oxidative stress, hyperproliferative response and renal carcinogenesis. Fe-NTA (9 mg Fe/kg body weight, intraperitoneally) enhances renal lipid peroxidation, xanthine oxidase, gamma-glutamyl transpeptidase and hydrogen peroxide (H2O2) generation with reduction in renal glutathione content, antioxidant enzymes and phase II metabolizing enzymes. It also caused increase in blood urea nitrogen, serum creatinine, ornithine decarboxylase (ODC) activity and thymidine [H] incorporation into renal DNA. It also enhanced DEN (N-diethylnitrosamine)-initiated renal carcinogenesis by increasing the percentage incidence of tumours. Treatment of rats orally with Nigella sativa (50 and 100 mg/kg body weight) resulted in significant decrease in gamma-glutamyl transpeptidase, lipid peroxidation, xanthine oxidase, H2O2 generation, blood urea nitrogen, serum creatinine, renal ODC activity, DNA synthesis (P<0.001) and incidence of tumours. Renal glutathione content (P<0.01), glutathione-metabolizing enzymes (P<0.001) and antioxidant enzymes were also recovered to significant levels (P<0.001). Thus, our data suggest that Nigella sativa is a potent chemopreventive agent and suppresses Fe-NTA-induced oxidative stress, hyperproliferative response and renal carcinogenesis in Wistar rats.

Article Published Date : Mar 31, 2005

Study Type : Animal Study

[117] Nigella sativa is protective against methotrexate induced nephrotoxicity

Abstract Title:

Effect of Nigella sativa (black seeds) against methotrexate-induced nephrotoxicity in mice.

Abstract Source:

J Intercult Ethnopharmacol. 2017 Jan-Mar;6(1):9-13. Epub 2016 Dec 22. PMID: 28163954

Abstract Author(s):

Jawad Hassan Ahmed, Isra Mohammed Abdulmajeed

Article Affiliation:

Jawad Hassan Ahmed

Abstract:

AIM: To evaluate the protective effect of Nigella sativa (NS) against nephrotoxicity of methotrexate (MTX) in mice.

MATERIALS AND METHODS: Four groups of Swiss albino male mice, eight in each group were used. The study was carried on between October 2014 and April 2015. Group 1 (control) were administered 0.3 ml distilled water orally daily for 21 days and injected with normal saline (0.25 ml) IP weekly. Group 2 (MTX group) were treated with MTX, 10 mg/kg IP weekly, while Group 3 were treated with 0.125 ml of NS oil by mouth daily and injected with normal saline (0.25 ml) IP weekly. Group 4 received 0.125 ml of NS oil by mouth daily and injected with 10 mg/kg MTX IP weekly. Oral treatments were administered using a special curved smooth tip nontraumatic metal needle and IP injections were given for 3 weeks at days 7, 14 and 21. Animals were sacrificed at day 23. Malondialdehyde (MDA) and glutathione (GSH) measurements were performed on kidney homogenate. Histopathology of the kidneys were prepared and examined.

RESULTS: MTX has resulted in a small elevation in MDA and reduction in GSH levels in kidney homogenate which was returned back to control values when NS and MTX were administered in combination. Statistical significance was achieved with elevation of GSH by MTX and NS compared to MTX alone. MTX caused histopathological changes suggesting nephrotoxicity in 6 animals out of 8, while no changes were found in all animals treated with MTX and NS.

CONCLUSION: NS is protective against MTX-induced nephrotoxicity.

Article Published Date : Dec 31, 2016

Study Type : Animal Study

[118] Nigella sativa L. oil protects against induced hepatotoxicity and improves serum lipid profile in rats

Abstract Title:

Nigella sativa L. oil protects against induced hepatotoxicity and improves serum lipid profile in rats.

Abstract Source:

Arzneimittelforschung. 2000 Sep ;50(9):832-6. PMID: 11050701

Abstract Author(s):

M el-Dakhakhny, N I Mady, M A Halim

Article Affiliation:

M el-Dakhakhny

Abstract:

The effects of 4 weeks oral intake of Nigella sativa L. (NS) oil on some liver function tests and D-galactosamine- or carbon tetrachloride-induced hepatotoxicity were investigated in male albino rats. In another series of experiments, the effect of the oil on serum lipid profile was examined in male spontaneously hypertensive rats of stroke prone strain and Wistar Kyoto rats. The study showed that daily administration of the oil per se (800 mg/kg orally for 4 weeks) did not adversely effect the serum transaminases (ALT and AST), alkaline phosphatase, serum bilirubin or prothrombin activity in normal albino rats. When the oil was given for 4 weeks prior to induction of hepatotoxicity by D-galactosamine or carbon tetrachloride, it was able to give complete protection against d-galactosamine and partial protection against carbon tetrachloride hepatotoxicity. NS oil showed a favourable effect on the serum lipid pattern where the administration of the oil (800 mg/kg orally for 4 weeks) caused a significant decrease in serum total cholesterol, low density lipoprotein, triglycerides and a significant elevation of serum high density lipoprotein level.

Article Published Date : Aug 31, 2000

Study Type : Animal Study

[119] Nigella sativa L. supplementation greatly improves lead-induced neurotoxicity in early life and provides neuroprotective and antioxidant potentials

Abstract Title:

Protective effects ofseed extract on lead induced neurotoxicity during development and early life in mouse models.

Abstract Source:

Toxicol Res (Camb). 2018 Jan 1 ;7(1):32-40. Epub 2017 Oct 5. PMID: 30090560

Abstract Author(s):

Umer Javed Butt, Syed Adnan Ali Shah, Touqeer Ahmed, Saadia Zahid

Article Affiliation:

Umer Javed Butt

Abstract:

Lead (Pb), a ubiquitous heavy metal and a known neurotoxicant, produces adverse effects on the brainincreased production of reactive oxygen species (ROS) and causes oxidative stress. In this study we examined the neuroprotective effects of the ethanolic extract ofseeds on Pb induced oxidative stress in the developing brain of mice. Mouse pups were exposed to low (0.1%) and high (0.2%) doses of Pb from the first day of pregnancy through their mothers (drinking water) and lactation until post-natal day (PND) 21. The mRNA expression levels of superoxide dismutase (SOD1), peroxiredoxin (Prdx6), amyloid precursor protein (APP) common, APP695 and APP770 were examined in the cortex and hippocampus of the mouse brain excised on PND 21 by semi-quantitative RT-PCR. The free radical scavenging activity of ethanolicextract was assessed by DPPH assay. The results showed that Pb exposure caused a significant decrease in the expression of SOD1, Prdx6 and APP695 and an increase in APP770 in both cortex and hippocampus in a dose dependent manner as compared to the control group. The expression of APP common remained unaltered. Histological assessment of the cortex and hippocampus demonstrated a decrease in the neuronal number and Nissl bodies. The administration of 250 and 500 mg kgethanolicextract reversed the adverse effects by significantly increasing the expression of SOD1, Prdx6 and APP695 and decreasing the expression of APP770 in both the regions. These results strongly suggest thatsupplementation greatly improves Pb-induced neurotoxicity in early life and provides neuroprotective and antioxidant potentials.

Article Published Date : Dec 31, 2017

Study Type : Animal Study

[120] Nigella sativa may be a beneficial agent in protection against ionizing radiation-related tissue injury

Abstract Title:

In vivo radioprotective effects of Nigella sativa L oil and reduced glutathione against irradiation-induced oxidative injury and number of peripheral blood lymphocytes in rats.

Abstract Source:

Photochem Photobiol. 2006 Nov-Dec;82(6):1691-6. PMID: 17387769

Abstract Author(s):

Mustafa Cemek, Hüseyin Enginar, Turan Karaca, Perihan Unak

Article Affiliation:

Mustafa Cemek

Abstract:

Radiotherapy is one of the most common therapies for treating human cancers. Several studies have indicated that irradiation induces reactive oxygen species (ROS), which play an important role in radiation damage of the cell. It has been shown that Nigella sativa L. (NS) and reduced glutathione (GSH) have both an antiperoxidative effect on different tissues and a scavenger effect on ROS. The purpose of this study was to determine the antioxidant and radio-protective roles of NS and GSH against irradiation-induced oxidative injury in an experimental model. The NS group was administrated NS (1 mL/kg body weight), the GSH group was injected GSH (150 mg/kg body weight) and the control group was given physiologic saline solution (1 mL/kg body weight) for 30 consecutive days before exposure to a single dose of 6 Gy of radiation. Animals were sacrificed after irradiation. Malondialdehyde, nitrate, nitrite (oxidative stress markers) and ascorbic acid, retinol, beta-carotene, GSH and ceruloplasmin (nonenzymatic antioxidant markers) levels and peripheral blood lymphocytes were measured in all groups. There were statistically significant differences between the groups for all parameters (P<0.05). Whole-body irradiation caused a significant increase in blood malondialdehyde, nitrate and nitrite levels. The blood oxidative stress marker levels in irradiated rats that were pretreated with NS and GSH were significantly decreased; however, non-enzymatic antioxidant levels were significantly increased. Also, our results suggest that NS and GSH administration prior to irradiation prevent the number of alpha-naphthyl acetate esterase peripheral blood T lymphocytes from declining. These results clearly show that NS and GSH treatment significantly antagonize the effects of radiation. Therefore, NS and GSH may be a beneficial agent in protection against ionizing radiation-related tissue injury.

Article Published Date : Oct 31, 2006

Study Type : Animal Study

[121] Nigella sativa may be clinically useful for protecting beta-cells against oxidative stress

Abstract Title:

Protective effects of the volatile oil of Nigella sativa seeds on beta-cell damage in streptozotocin-induced diabetic rats: a light and electron microscopic study.

Abstract Source:

J Mol Histol. 2009 Oct ;40(5-6):379-85. Epub 2010 Jan 5. PMID: 20049514

Abstract Author(s):

Mehmet Kanter, Meryem Akpolat, Cevat Aktas

Article Affiliation:

Mehmet Kanter

Abstract:

The aim of this study was to evaluate the possible protective effects of the volatile oil of Nigella sativa (NS) seeds on insulin immunoreactivity and ultrastructural changes of pancreatic beta-cells in STZ-induced diabetic rats. STZ was injected intraperitoneally at a single dose of 50 mg/kg to induce diabetes. The rats in NS treated groups were given NS (0.2 ml/kg) once a day orally for 4 weeks starting 3 days prior to STZ injection. To date, no ultrastructural changes of pancreatic beta-cells in STZ induced diabetic rats by NS treatment have been reported. Islet cell degeneration and weak insulin immunohistochemical staining was observed in rats with STZ-induced diabetes. Increased intensity of staining for insulin, and preservation of beta-cell numbers were apparent in the NS-treated diabetic rats. The protective effect of NS on STZ-diabetic rats was evident by a moderate increase in the lowered secretory vesicles with granules and also slight destruction with loss of cristae within the mitochondria of beta-cell when compared to control rats. These findings suggest that NS treatment exerts a therapeutic protective effect in diabetes by decreasing morphological changes and preserving pancreatic beta-cell integrity. Consequently, NS may be clinically useful for protecting beta-cells against oxidative stress.

Article Published Date : Sep 30, 2009

Study Type : Animal Study

[122] Nigella sativa oil (NSO) and its active component, TQ, clearly protect brain tissue from radiation-induced nitrosative stress

Abstract Title:

Radiation-modifying abilities of Nigella sativa and Thymoquinone on radiation-induced nitrosative stress in the brain tissue.

Abstract Source:

Phytomedicine. 2013 Nov 21. pii: S0944-7113(13)00432-7. doi: 10.1016/j.phymed.2013.10.023. [Epub ahead of print]

Abstract Author(s):

Adem Ahlatci, Abdurahman Kuzhan, Seyithan Taysi, Omer Can Demirtas, Hilal Eryigit Alkis, Mehmet Tarakcioglu, Ali Demirci, Derya Caglayan, Edibe Saricicek, Kadir Cinar

Article Affiliation:

Department of Radiation Oncology, Gaziantep University, Medical School, Gaziantep, Turkey.

Abstract:

To investigate Nigella sativa oil (NSO) and Thymoquinone (TQ) for their antioxidant effects on the brain tissue of rats exposed to ionizing radiation. Fifty-four male albino Wistar rats, divided into six groups, were designed as group I (normal control group) did not receive NSO, TQ or irradiation; group II (control group of TQ) received dimethyl sulfoxide and sham irradiation; group III (control group of NSO) received saline and sham irradiation; group IV (irradiation plus NSO group) received both 5 Gray of gamma irradiation to total cranium and NSO; group V (irradiation plus TQ group) received both irradiation and TQ; group VI (irradiation alone group) received irradiation plus saline. Alterations in nitric oxide (NO) and peroxynitrite (ONOO(-)) levels, and nitric oxide synthase (NOS) enzyme activity were measured by biochemical methods in homogenized brain tissue of rats. Levels of NO and ONOO(-), and enzyme activity of NOS in brain tissue of the rats treated with NSO or TQ were found to be lower than in received IR alone (p<0.002) Nigella sativa oil (NSO) and its active component, TQ, clearly protect brain tissue from radiation-induced nitrosative stress.

Article Published Date : Nov 20, 2013

Study Type : Animal Study

[123] Nigella sativa oil ameliorates sodium nitrite-induced nephrotoxicity in animals

Abstract Title:

Nigella sativa oil attenuates chronic nephrotoxicity induced by oral sodium nitrite: Effects on tissue fibrosis and apoptosis.

Abstract Source:

Redox Rep. 2015 Jul 29. Epub 2015 Jul 29. PMID: 26221999

Abstract Author(s):

Mohammed M H Al-Gayyar, Hanan M Hassan, Abdullah Alyoussef, Ahmed Abbas, Mohamed M Darweish, Amany A El-Hawwary

Article Affiliation:

Mohammed M H Al-Gayyar

Abstract:

Objectives Sodium nitrite, a food preservative, has been reported to increase oxidative stress indicators such as lipid peroxidation, which can affect different organs including the kidney. Here, we investigated the toxic effects of oral sodium nitrite on kidney function in rats and evaluated potential protective effects of Nigella sativa oil (NSO). Methods Seventy adult male Sprague-Dawley rats received 80 mg/kg sodium nitrite orally in the presence or absence of NSO (2.5, 5, and 10 ml/kg) for 12 weeks. Morphological changes were assessed by hematoxylin and eosin, Mallory trichome, and periodic acid-Schiff staining. Renal tissues were used for measurements of oxidative stress markers, C-reactive protein, cytochrome C oxidase, transforming growth factor (TGF)-beta1, monocyte chemotactic protein (MCP)-1, pJNK/JNK, and caspase-3. Results NSO significantly reduced sodium nitrite-induced elevation in serum urea and creatinine, as well as increasing normal appearance of renal tissue. NSO also prevented reductions in glycogen levels caused by sodium nitrite alone. Moreover, NSO treatment resulted in dose-dependent significant reductions in fibrosis markers after sodium nitrite-induced 3- and 2.7-fold increase in MCP-1 and TGF-beta1, respectively. Finally, NSO partially reduced the elevated caspase-3 and pJNK/JNK. Discussion NSO ameliorates sodium nitrite-induced nephrotoxicity through blocking oxidative stress, attenuation of fibrosis/inflammation, restoration of glycogen level, amelioration of cytochrome C oxidase, and inhibition of apoptosis.

Article Published Date : Jul 28, 2015

Study Type : Animal Study

[124] Nigella sativa oil and chromium picolinate monotherapy can reduce fructose-induced insulin resistance

Abstract Title:

Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats.

Abstract Source:

Biol Trace Elem Res. 2017 Oct 4. Epub 2017 Oct 4. PMID: 28975521

Abstract Author(s):

Mohamed Mahmoud Elseweidy, Rawia Sarhan Amin, Hebatallah Husseini Atteia, Maha Abdo Aly

Article Affiliation:

Mohamed Mahmoud Elseweidy

Abstract:

In vivo and in vitro studies suggested that chromium enhances insulin sensitivity by promoting insulin receptor signaling. However, its effect on insulin clearance has not been yet identified. Nigella sativa, a widely used spice, possesses an antidiabetic activity. We, therefore, hypothesized that chromium picolinate may alter insulin clearance by modulating insulin-degrading enzyme (IDE) in insulin-resistant rats. We evaluated also the effect of Nigella sativa oil on insulin signaling and degradation with respect to chromium picolinate. To assess these hypotheses, insulin resistance was induced in 30 male Wistar albino rats through daily oral administration of high-fructose water (HFW, 20% w/v) for 45 days. These rats were then divided into three groups (n = 10/group). They were given either no treatment (control group) or Nigella sativa oil (500 mg/kg bw/day) or chromium picoloinate (200 μg/kg bw/day) orally along with HFW (20% w/v) for 45 days. Nigella sativa oil or chromium picolinate concurrent administration with HFW significantly decreased body weight, serum lipids, glucagon, insulin resistance, and hepatic IDE level but increased its mRNA expression and insulin receptor phosphorlyation as well as high-density lipoprotein cholesterol (HDL-C) level as compared to control group values, suggesting their potential as modulators for insulin signaling and clearance. However, Nigella sativa oil exerted better improvement in feeding efficacy ratio as well as the levels of glucagon, insulin, insulin resistance, hepatic IDE level and insulin receptor phosphorylation than chromium picolinate, suggesting its greater insulin sensitizing capacity. Our data, for the first time, prove that Nigella sativa oil and chromium picolinate monotherapy can reduce fructose-induced insulin resistance by reduction of hepatic IDE protein and activation of insulin receptor signaling.

Article Published Date : Oct 03, 2017

Study Type : Animal Study

[125] Nigella sativa oil and Thymoquinone correct STZ-diabetes-induced alterations in CK-MB and brain monoamines due to their antioxidant properties

Abstract Title:

Effects of Nigella sativa oil and thymoquinone on oxidative stress and neuropathy in streptozotocin-induced diabetic rats.

Abstract Source:

Pharmacology. 2009 ;84(3):127-34. Epub 2009 Aug 13. PMID: 19684436

Abstract Author(s):

Nadia M Hamdy, Ragia A Taha

Article Affiliation:

Nadia M Hamdy

Abstract:

We examined whether Nigella sativa (NS) oil and its active constituent thymoquinone (TQ) attenuate oxidative stress in the heart and brain in an experimental model of diabetes mellitus using streptozotocin (STZ). Oxidative stress was assessed by measuring cardiac and brain nitric oxide (NO), lipid peroxide levels, glutathione (GSH) and antioxidant enzyme activities, i.e. glutathione-S-transferase (GST) and catalase. Cardiac metabolic damage was estimated by measuring cardiac creatine kinase muscle and brain types (CK-MB). Brain monoamine levels were also evaluated. STZ diabetes induced a significant increase in heart and brain NO and malondialdehyde concentrations compared with the control group. These changes were attenuated by posttreatment of rats with NS oil and TQ. STZ diabetes induced oxidative stress via a significant decrease in GST, GSH and catalase. These lowered levels were improved by either NS oil or TQ administration. Serum CK-MB was decreased in the diabetic rats, which recovered with NS oil and TQ administration. During the course of diabetes, there was a marked increase in norepinephrine and dopamine concentrations and a marked decrease in serotonin concentration compared to the control group. These findings were partly reversed by oral administration of either NS oil or TQ. It is concluded that NS and TQ correct STZ-diabetes-induced alterations in CK-MB and brain monoamines due to their antioxidant properties.

Article Published Date : Dec 31, 2008

Study Type : Animal Study

[126] Nigella sativa oil and thymoquinone have protective effects against toxicity induced by the anticancer drug cyclophosphamide

Abstract Title:

Protective effects of Nigella sativa oil and thymoquinone against toxicity induced by the anticancer drug cyclophosphamide.

Abstract Source: